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Method for performing extraction separation on choerospondias

A technology of jujube jujube and extract solution, which is applied in the field of extraction and separation of effective components of natural plants, can solve the problems that there is no report on the extraction and purification method of jujube jujube with high purity, shorten the extraction cycle and the amount of solvent, and achieve simple process and stable quality Effect

Inactive Publication Date: 2013-08-14
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After literature search, there is no report on the extraction and purification method suitable for high-purity jujube glycoside

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dry and pulverize the bark of Jujube jujuba at low temperature, take 5kg, place it in three series-connected extraction devices on average, add 20L of 70% ethanol solution, and extract twice at an extraction temperature of 50°C for 2 hours each time, and combine the extracts After filtration, the extract was obtained, concentrated by rotation, dispersed with water, filtered, and the filtrate passed through the polyamide resin column. After the adsorption was completed, the impurities were first eluted with 4BV of 25% ethanol, and then the active ingredients were eluted with 7BV of 75% ethanol, and the target washing was collected. Deliquoring, concentrating and drying to obtain the crude product of jujube saponin, the obtained crude product is separated and purified by high-speed countercurrent chromatography, and the n-heptane-ethyl acetate-methanol-water solvent system is prepared according to the volume ratio of 16:13:25:7, and the solvent After mixing the system, let...

Embodiment 2

[0022] Dry and pulverize the bark of Jujube jujube at low temperature, take 5kg, put it in three extraction devices connected in series on average, add 18L of 65% ethanol solution, extract three times at the extraction temperature of 50°C, each time for 1.5h, and combine the extraction After filtration, the extract was obtained, concentrated by rotation, dispersed with water, filtered, and the filtrate passed through the polyamide resin column. After the adsorption was completed, the impurities were first eluted with 3.5BV of 20% ethanol, and then the active ingredients were eluted with 6BV of 80% ethanol. The target eluate was concentrated and dried to obtain the crude product of jujube glycoside, and the obtained crude product was separated and purified by high-speed countercurrent chromatography, and the n-heptane-ethyl acetate-methanol-water solvent system was prepared at a volume ratio of 14:11:22:5 , the solvent system is mixed evenly and allowed to stand for stratificati...

Embodiment 3

[0024] Dry and pulverize the bark of Jujube jujuba at low temperature, take 5kg, put it in 5 extraction devices connected in series on average, add 20L of 80% ethanol solution, extract 3 times at the extraction temperature of 50°C, 2 hours each time, and combine the extracts After filtration, the extract was obtained, concentrated by rotation, dispersed with water, filtered, and the filtrate passed through a polyamide resin column. After the adsorption was completed, the impurities were first eluted with 4BV of 25% ethanol, and then the active ingredients were eluted with 6BV of 75% ethanol, and the target washing was collected. Deliquidation, concentration and drying to obtain the crude product of jujube saponin, the obtained crude product is separated and purified by high-speed countercurrent chromatography, and the n-heptane-ethyl acetate-methanol-water solvent system is prepared according to the volume ratio of 17:13:23:6, and the solvent After mixing the system, let it sta...

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PUM

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Abstract

The invention relates to a method for performing extraction separation on choerospondias. The method comprises the following steps that: choerospondias axillaris bark is smashed and subjected to multi-tank dynamic countercurrent extraction; an extracting solution is filtered and subjected to rotary concentration; a concentrated solution is dispersed by adding water, and subjected to polyamide resin column adsorption and alcohol-water solution elution; an eluent is concentrated; and choerospondias is obtained through separation and purification by a semi-preparation high-speed countercurrent chromatograph. The method is simple in technological process, high in extraction efficiency, small in pollution and low in cost, and greatly facilitates industrial production; and the purity of prepared choerospondias reaches above 97%.

Description

technical field [0001] The invention belongs to the field of extracting and separating effective components of natural plants, and relates to a method for jujube glycosides, in particular to a method for extracting and separating jujube glycosides by using a combination of dynamic countercurrent extraction, polyamide resin column adsorption and high-speed countercurrent chromatographic purification technology method. Background technique [0002] Choerospondin is a flavonoid glycoside compound, which is mainly isolated from the bark of Choerospondias axillaris (Roxb.) Burtt et Hill, Anacardiaceae. Pharmacological studies have shown that jujube glycoside has antibacterial effect. It has inhibitory effect on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and nitrate-negative bacilli; it also has inhibitory effect on aldose reductase. The molecular formula of jujube glycoside is C 21 h 22 o 10 , the molecular weight is 434.4, and the structural formula is:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/08
Inventor 苏刘花万冬梅
Owner NANJING ZELANG AGRI DEV