Fusion method of pleurotus nebrodensis protoplast and pleurotus eryngii protoplast

Abstract

The invention provides a fusion method of a pleurotus nebrodensis protoplast and a pleurotus eryngii protoplast. The fusion method is characterized in that hyphal cells of pleurotus nebrodensis and pleurotus eryngii are subjected tot enzymatic hydrolysis for preparation of the protoplasts, the pleurotus nebrodensis protoplast is subjected to the heat inactivation to serve as a mark, and the pleurotus eryngii protoplast is subjected to ultraviolet inactivation to serve as a mark, the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast are fused by adopting a PEG (Polyethylene Glycol) mediated chemical fusion method, and a fused strain is comprehensively tested through antagonistic experiments, bacterial colony phenotypes, hypha shapes and fruiting experiments in combination of RAPD (random amplified polymorphic DNA). The fusion method can be used for effectively fusing the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast to obtain a new bacterial strain, the pileus of which is not easy to break and the fruiting body of which is individual; and an effective method is provided for breeding a target bacterial strain through the fusion of the protoplasts.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding of edible fungi, and in particular relates to a method of combining the protoplasts of Pleurotus eryngii and Pleurotus eryngii to obtain a cap that is not fragile and has solitary fruiting bodies that have both the advantages of Pleurotus eryngii and Pleurotus eryngii strain method. Background technique [0002] At present, most of the traditional edible fungus breeding methods are artificial mutation breeding, and there is no report on the fusion of Bailing mushroom and Pleurotus eryngii protoplasts. It has the advantages of strong drought, anti-cancer and anti-cancer, prevention and treatment of cardiovascular disease in the elderly, gynecological swelling and pain, rickets and rickets in children, etc., but its ability to decompose lignin, cellulose and protein is very weak. Pleurotus eryngii is a fungus with strong ability to decompose lignin, cellulose, and protein. It is rich in vitami...

AI Technical Summary

Problems solved by technology

[0004] At present, the breeding of edible fungi is mostly artificial mutagenesis. Due to the non-directional characteristics of mutagenesis, the effect of solving the above problems is not good. There is no report on the fusion of Pleurotus eryngii and Pleurotus eryngii protoplasts at home and abroad.

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