Fusion method of pleurotus nebrodensis protoplast and pleurotus eryngii protoplast

A protoplast fusion and protoplast technology, applied in the preparation of hybrid cells, etc., can solve problems such as poor effect, achieve the effect of strong repeatability, benefit food quality and safety, and improve the probability of success

Inactive Publication Date: 2013-08-21
TIANJIN NORMAL UNIVERSITY
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the breeding of edible fungi is mostly artificial mutagenesis. Due to the non-directional characteristics of mutagenesis, the effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fusion method of pleurotus nebrodensis protoplast and pleurotus eryngii protoplast
  • Fusion method of pleurotus nebrodensis protoplast and pleurotus eryngii protoplast
  • Fusion method of pleurotus nebrodensis protoplast and pleurotus eryngii protoplast

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1

[0049] (1) Take the front end of the vigorous mycelium on the PDA comprehensive medium and inoculate it in a 100 mL Erlenmeyer flask containing Pleurotus ostreatus liquid medium. The volume of the bottle is 50 mL, and each bottle receives 3 to 4 0.5 cm clumps. Incubate in the dark at 25°C for 5-11 days; pick out the mycelium under aseptic conditions and absorb the water with sterile filter paper to obtain fresh Pleurotus ostreatus mycelium.

[0050] (2) Take fresh Pleurotus eryngii and Pleurotus eryngii mycelium, and carry out enzymolysis at the ratio of 0.1g wet weight and 1ml enzyme solution. Among them, the enzymatic hydrolysis temperature of Pleurotus eryngii mycelium protoplast is 34℃ and the enzymatic hydrolysis time is 3h. The enzymatic hydrolysis temperature of Pleurotus eryngii mycelium protoplasts is 34°C, and the hydrolysis time is 2h. The enzymatic hydrolysis of Pleurotus eryngii mycelium according to the present invention: 2ml 0.6mol / L MgSO 4 ·7H 2 O...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a fusion method of a pleurotus nebrodensis protoplast and a pleurotus eryngii protoplast. The fusion method is characterized in that hyphal cells of pleurotus nebrodensis and pleurotus eryngii are subjected tot enzymatic hydrolysis for preparation of the protoplasts, the pleurotus nebrodensis protoplast is subjected to the heat inactivation to serve as a mark, and the pleurotus eryngii protoplast is subjected to ultraviolet inactivation to serve as a mark, the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast are fused by adopting a PEG (Polyethylene Glycol) mediated chemical fusion method, and a fused strain is comprehensively tested through antagonistic experiments, bacterial colony phenotypes, hypha shapes and fruiting experiments in combination of RAPD (random amplified polymorphic DNA). The fusion method can be used for effectively fusing the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast to obtain a new bacterial strain, the pileus of which is not easy to break and the fruiting body of which is individual; and an effective method is provided for breeding a target bacterial strain through the fusion of the protoplasts.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding of edible fungi, and in particular relates to a method of combining the protoplasts of Pleurotus eryngii and Pleurotus eryngii to obtain a cap that is not fragile and has solitary fruiting bodies that have both the advantages of Pleurotus eryngii and Pleurotus eryngii strain method. Background technique [0002] At present, most of the traditional edible fungus breeding methods are artificial mutation breeding, and there is no report on the fusion of Bailing mushroom and Pleurotus eryngii protoplasts. It has the advantages of strong drought, anti-cancer and anti-cancer, prevention and treatment of cardiovascular disease in the elderly, gynecological swelling and pain, rickets and rickets in children, etc., but its ability to decompose lignin, cellulose and protein is very weak. Pleurotus eryngii is a fungus with strong ability to decompose lignin, cellulose, and protein. It is rich in vitami...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/04
Inventor 郭成金刘西周
Owner TIANJIN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap