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A kind of chicken infectious bursal disease live vaccine and production method thereof

A technology for chicken infectious and bursal disease, which is applied in the directions of antiviral agents, viral antigen components, freeze-dried delivery, etc., can solve the problems of onset and the difficulty of determining the optimal immunization time.

Active Publication Date: 2014-10-22
北京中海生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the great interference of maternal antibodies on live vaccines, the initial immunization time must be determined according to the level of maternal antibodies to ensure the immune effect of the vaccine. In actual production, different individual maternal antibody levels in the same flock also exist There is a large difference, and the optimal immunization time is not easy to determine. Once the immunization time is inappropriate, wild poisons often take advantage of the emptiness and cause disease.

Method used

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  • A kind of chicken infectious bursal disease live vaccine and production method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] - vaccine manufacturing

[0053] 1. Materials

[0054] (1) Virus content of chicken infectious bursal disease virus CF-C strain 10 5.25 ELD 50 / 0.2ml, prepared and preserved by the inventor.

[0055] (2) Pig anti-IBDV hyperimmune serum was prepared by the inventors.

[0056] (3) The highly virulent chicken infectious bursal disease BC6-85 strain used for inspection was provided by the China Veterinary Drug Control Institute.

[0057] (4) SPF chicken embryos were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.

[0058] (5) SPF chickens were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.

[0059] 2. Preparation of seed poison for production

[0060] (1) Chicken infectious bursal disease virus CF-C strain

[0061] 1) Inoculation and Harvesting Dilute the seed virus solution of CF-C strain to 100 times (volume ratio), inoculate on the chorioallantoic membrane of 10-day-old chicken embryos, 0.2ml per embryo,...

Embodiment 2

[0076] ——Finished product inspection (take 1301 batches of vaccines as an example)

[0077] 1. Properties The vaccine is a milky white spongy loose mass, which is easy to detach from the bottle wall and dissolves quickly after adding diluent.

[0078] 2. The sterility test of the vaccine is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there is no growth of bacteria and mold.

[0079] 3. Mycoplasma inspection The vaccine is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there is no mycoplasma growth.

[0080] 4. Exogenous virus inspection The vaccine is tested according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and there is no exogenous virus contamination.

[0081] 5. Safety inspection Dilute the vaccine to 10 feathers / 0.1ml, inoculate 18-day-old SPF chicken embryos (30 pieces) in the embryo and subcutaneously inoculate 1-day-old SPF chickens (20 pieces) in t...

Embodiment 3

[0086] ——Comparative test with similar products

[0087] The safety and immunoefficacy of foreign chicken infectious bursal disease (2512 strains) single vaccines of the same serotype as CF-C strains were compared with 201201 batches of vaccines produced in the laboratory.

[0088] The 1-day-old broiler chickens with different maternal antibody levels were randomly divided into 3 groups, 30 chickens in each group, labeled, blood collected, and antibody titer measured. Dilute the 201201 batch of vaccines in the laboratory to 1 feather / 0.1ml, and inoculate 30 1-day-old broilers subcutaneously through the neck, 0.1ml / piece. Dilute the single vaccine to 1 feather / 0.1ml according to the instructions, instill eye drops, 0.1ml / bird, and keep 30 non-immunized as a control, and keep them in isolation under the same conditions.

[0089] On the 14th, 21st, and 28th days after immunization, blood was collected from 10 rats in each immunization group and 5 rats in the control group for an...

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Abstract

The invention relates to a production method of a chicken infectious bursal disease live vaccine. A virulent strain having the characteristics of good immunogenicity, broad antigen spectrum and immune synergistic effect is screened out through the researches on the characteristics comprising the immunogenicity, the antigen spectrum, the pathogenicity to the bursa of Fabricius, the maternal antibody breakthrough capability and the like of different subtype chicken infectious bursal viruses to develop the researches on chicken infectious bursal disease live vaccines in order to produce the chicken infectious bursal disease trivalent live vaccine which has a good immunogenicity, can breakthrough high-level maternal antibodies, can continuously immunize for above 3 months and can well protect different virulent strains.

Description

technical field [0001] The invention relates to a chicken infectious bursal disease live vaccine and a production method thereof. It belongs to the field of veterinary biological products. Background technique [0002] Chicken infectious bursal disease is an acute, highly contagious, lymphocytic disease of chicks caused by a double-stranded RNA virus. The main measure is immunization with vaccines. Due to the great interference of maternal antibodies on live vaccines, the initial immunization time must be determined according to the level of maternal antibodies to ensure the immune effect of the vaccine. In actual production, different individual maternal antibody levels in the same flock also exist There is a large difference, and the optimal immunization time is not easy to determine. Once the immunization time is inappropriate, wild poisons often take advantage of the emptiness and cause disease. In recent years, IBD vaccine research is in the ascendant, and many new v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/42A61K39/12A61K9/19A61P31/14
Inventor 蒋桃珍陈光华孙晔
Owner 北京中海生物科技有限公司