Preparation method of bone repair scaffold with osteoid porous regenerative channel structure

Abstract

The invention discloses a preparation method of a bone repair scaffold with an osteoid pore-like regenerative channel structure. Embed the fibrous pore-forming material with a diameter corresponding to the required pore diameter in the nano-apatite-polyamide composite material slurry with a viscosity of 50-500Pa.s, and evaporate the solvent by heating at 80-200°C After curing, the fibrous pore-forming material therein is removed to obtain a bone repair scaffold product with oriented pores / channels in the structure. The method is simple, can prepare bone repair scaffolds with oriented pore channels, and can adjust the pore size of the prepared osteoid oriented pore channels and the porosity of the scaffold according to the needs, and can significantly improve the mechanical properties of the oriented pore channels in the scaffold. Good performance, the permeability of the pores is good, which is conducive to rapid vascularization, cells and new bone tissue growth, and promotes the regeneration and repair of bone tissue.

Description

technical field [0001] The invention relates to a method for preparing a bone-like apatite-polyamide composite material porous bone repair bracket with a bone-like porous regeneration channel structure. Background technique [0002] As a porous scaffold for bone repair tissue, its three-dimensional structure not only plays an important role in the selective permeability of cells, nutrients and oxygen, the removal of metabolic substances and the survival of new tissue, but also greatly affects the cell behavior and tissue reconstruction. Appropriate pore structure is the key to the optimal osteogenesis performance of porous scaffolds in bone tissue engineering. [0003] Studies have found that many tissues in the human body, including nerves, muscles, tendons, blood vessels, bones and teeth, have ordered anisotropic structures. This anisotropy is not only reflected in the structure, but also in the physiological function and mechanical properties. For example, bone tissue i...

AI Technical Summary

Problems solved by technology

Traditional bone tissue engineering scaffolds have an irregular isotropic pore structure with no regularity and direction orientation. The large pores are often connected only through small pores of a few microns to more than ten microns, and the pore penetration is relatively low. Low, the common problem is that new bone tissue is often formed on the outer edge of the scaffold, making it difficult for cells to penetrate into the interior of the scaffold, hindering the exchange of body fluids inside the scaffold, and eventually leading to apoptosis of cells inside the scaffold and necrosis of new tissue etc., affecting the reconstruction of tissue in large defect areas

Although these anisotropic structural scaffolds have been shown to have good characteristics and prospects in the field of tissue engineering, the current common problem of scaffolds used for bone repair is that their mechanical properties are poor, and they are not as good as mechanical properties and vascularization. Scaffold materials with bone conduction or induction can meet the needs of bone repair in clinical load-bearing parts

The scaffold pores prepared by these methods have a disordered structure, a high rate of closed pores, poor connectivity between pores, and it is difficult for the new bone tissue of the nucleus to grow into the interior of the scaffold, which affects its application effect.

In addition, such methods may leave chemical foaming agents that are not completely decomposed or removed, which will have adverse effects on the matrix or local tissues

Wang HN, et al. reported the preparation of nanoapatite-polyamide composite scaffolds with oriented pore structure by thermally induced phase transfer (J Polym Sci Part A: Polym Chem, 2008, 47: 658-699), but The stent prepared by this method has an orientation hole structure only within a few millimeters (about 3mm), and the pore size of the orientation hole is difficult to control, so it cannot be used in practice.

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