Target SERS (Surface Enhanced Raman Scattering) probe with pH-sensitive drug release characteristic, and preparation method thereof

A probe and sensitive technology, applied in the field of medicine and biology, can solve problems such as photobleaching, restricted application, spectral overlap, etc., and achieve the effect of increasing translocation and increasing savings

Inactive Publication Date: 2013-09-04
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fluorescence imaging still has problems such as spectral overlap and photobleaching caused by emission spectral width, which restricts its application in some fields.

Method used

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  • Target SERS (Surface Enhanced Raman Scattering) probe with pH-sensitive drug release characteristic, and preparation method thereof
  • Target SERS (Surface Enhanced Raman Scattering) probe with pH-sensitive drug release characteristic, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A targeted SERE probe with pH-sensitive drug release characteristics has a core-shell structure, and the probe sequentially includes a gold nanoparticle core labeled with Raman molecules, a silica intermediate layer, and a mesoporous diatom core from the inside to the outside. A shell layer of a silicon oxide layer, a pH-sensitive polymer layer, and a targeting ligand on the surface of the shell layer, the pH-sensitive polymer layer is a chitosan / polymethacrylic acid layer, and the targeting ligand is transferrin . The Raman molecule is 4-mercaptobenzoic acid (4MBA) molecule. The mesoporous silica layer is loaded with drug doxorubicin (DOX). The structure of the targeted SERE probe is spherical, its particle size is 120nm, and its shell thickness is 80nm.

[0029] figure 1 Shown is that the targeted SERS probe with pH-sensitive drug release characteristics has a core-shell structure, the core body is gold nanoparticles, the middle silica layer and mesoporous silica l...

Embodiment 2

[0037] A targeted SERE probe with pH-sensitive drug release characteristics has a core-shell structure, and the probe sequentially includes a gold nanoparticle core labeled with Raman molecules, a silica intermediate layer, and a mesoporous diatom core from the inside to the outside. A shell layer of a silicon oxide layer, a pH-sensitive polymer layer, and a targeting ligand on the surface of the shell layer, the pH-sensitive polymer layer is a chitosan / polymethacrylic acid layer, and the targeting ligand is transferrin . The Raman molecule is 5,5-dithiobis(2-nitrobenzoic acid) molecule. The mesoporous silicon dioxide layer is loaded with drug mitoxantrone. The targeted SERE probe structure is spherical, its particle size is 160 nm, and its shell thickness is 120 nm.

[0038] Using 5,5-dithiobis(2-nitrobenzoic acid) molecule as SERS marker, mitoxantrone as drug molecule, transferrin as targeting ligand to recognize tumor cells, targeting SERE probe The preparation method co...

Embodiment 3

[0045] It is basically the same as Example 1, except that the targeted SERE probe has a spherical structure, a particle size of 140 nm, and a shell thickness of 100 nm.

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Abstract

The invention discloses a target SERS (Surface Enhanced Raman Scattering) probe with pH-sensitive drug release characteristic. The probe comprises a Raman molecule-labeled gold nanoparticle core, a silicon dioxide intermediate layer, a mesoporous silica layer, a pH-sensitive polymer layer, a shell layer and a target ligand on the surface of the shell layer sequentially from inside to outside, the pH-sensitive polymer layer is a chitosan / polymethylacrylic acid layer, and the target ligand is transferrin. The invention further discloses a preparation method for the SERS probe. The probe and the preparation method thereof have the advantages that a pH-sensitive drug release system is combined with SERS, and the target ligand is connected with target tumor cells; the diffusion and release speed of drug in nano-particles can be regulated and controlled according to the pH value of the environment; the tracking on the nano-drug carrier particles can be more accurate through SERS signals in nano-drug carriers; and the target ligand is used for increasing the accumulation of drug in tumor parts, and increasing the transfer of drug in tumor cells.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a targeted SERS probe with pH-sensitive drug release characteristics and a preparation method thereof. Background technique [0002] With the continuous development of nanomaterial preparation technology, nano drug carriers are gradually becoming the focus of domestic and foreign researchers. Among them, due to its large pore volume and specific surface area, good biocompatibility, non-toxicity and no pharmacological activity, mesoporous silicon nanomaterials can achieve high It is an ideal drug carrier material. [0003] Smart drug carriers are able to respond to external environmental stimuli, such as temperature, solvent composition, pH value, stress, electric field, and small changes in specific molecules or ions, and their volumes undergo discontinuous changes, that is, volume phase transitions. The human body is a complex environmental system, each part has different ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K47/02A61K47/20A61K47/04A61K47/36A61K47/32A61K49/00A61K31/704A61K31/136A61P35/00
Inventor 崔一平方薇王著元宗慎飞
Owner SOUTHEAST UNIV
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