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Method for improving banana immature male flower embryonic callus induction success rate

An embryogenic callus, success rate technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as easy browning and necrosis, and achieve the effect of reducing harm

Inactive Publication Date: 2013-09-11
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Male flowers in the first to eighth rows are prone to browning and necrosis during cultivation on the induction medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A. Select the triploid bananas that are currently widely promoted in production and difficult to establish embryogenic suspension cell lines as starting materials, including: Musa acuminate AAA Cavendish cv.Brazil, Williams B6, Haijiao No. 1 , Anaikomban, Matti, etc.

[0033] B. Induction of embryogenic callus

[0034] (1) Select male flower buds from 1 to 10 weeks after flowering, peel off the outer bracts of the buds under aseptic conditions until the size of the buds is 0.8cm×2cm, put the buds into a plastic box with water drops on the inner wall, The plastic box is sealed with parafilm until the buds are removed for surface sterilization;

[0035] (2) Take out the above-mentioned buds from the plastic box, soak them in 70% alcohol for 1 minute, then take them out and put them on the ultra-clean workbench for later use;

[0036] (3) In the ultra-clean workbench, the flower buds after surface sterilization are placed in a sterile petri dish with a diameter of 120 cm...

Embodiment 2

[0051] A. Select triploid bananas that are currently difficult to establish embryonic suspension cell lines as starting materials, including: high-species Tianbao banana, Gaojiaodun mine, Qiwei, Hekou Gaoba banana, Xianren banana, Bantam landmine, Guangdong Banana No. 2 and more.

[0052] B. Induction of embryogenic callus

[0053] (1) Select male flower buds from 1 to 10 weeks after flowering, peel off the outer bracts of the buds under aseptic conditions until the size of the buds is 0.8cm×2cm, put the buds into a plastic box with water drops on the inner wall, The plastic box is sealed with parafilm until the buds are removed for surface sterilization;

[0054] (2) Take out the above-mentioned buds from the plastic box, soak them in 70% alcohol for 1 minute, then take them out and put them on the ultra-clean workbench for later use;

[0055] (3) In the ultra-clean workbench, the flower buds after surface sterilization are placed in a sterile petri dish with a diameter of ...

Embodiment 3

[0070] A. Select triploid bananas that are currently difficult to establish embryogenic suspension cell lines as starting materials, including: Guangdong Banana No. 1, Guangdong Dazhong Aibao, Dongguan Zhongba, Zhongshan Yajiao, Yunnan Hekou Zhongba, Guangxi Longzhou Zhongba Put, Gaozhou dwarf banana, Guangxi Nalong banana, Yunnan Honghe dwarf banana, Saigon banana, etc.

[0071] B. Induction of embryogenic callus

[0072] (1) Select male flower buds from 1 to 10 weeks after flowering, peel off the outer bracts of the buds under aseptic conditions until the size of the buds is 0.8cm×2cm, put the buds into a plastic box with water drops on the inner wall, The plastic box is sealed with parafilm until the buds are removed for surface sterilization;

[0073] (2) Take out the above-mentioned buds from the plastic box, soak them in 70% alcohol for 1 minute, then take them out and put them on the ultra-clean workbench for later use;

[0074] (3) In the ultra-clean workbench, the f...

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Abstract

The invention discloses a method for improving the banana immature male flower embryonic callus induction success rate, and relates to a method for establishing a banana embryonic suspension cell system. The method comprises the following steps of: A, selecting a banana variety which is widely popularized at present or a banana variety which hardly induces embryonic callus; B, selecting banana flower buds which bloom for 1-10 weeks, and preserving the flower buds for 24 hours before immature male flowers are officially cultivated; C, carrying out embryonic callus induction; D, obtaining embryonic suspension cells; E, developing young embryos; and F. regenerating the young embryos into seedlings. According to the method, the purpose of improving the induction success rate of the banana immature male flower embryonic callus and the establishment success rate of the banana embryonic suspension cell system is achieved by using different culture medium formulae and culture processes at different key stages of inducing the banana immature male flower embryonic callus and establishing the banana embryonic suspension cell system; due to the application of the method, embryonic suspension cell systems of a plurality of triploid banana varieties which are widely popularized are effectively established, and the development of transgenosis of different banana varieties is facilitated.

Description

technical field [0001] The invention relates to a banana somatic cell regeneration method, in particular to a method for obtaining embryogenic suspension cells by increasing the success rate of embryogenic callus induction, and generating banana seedlings from the embryogenic suspension cells. Background technique [0002] In the process of banana planting in my country, problems such as fusarium wilt, freezing damage, typhoon, and post-harvest rot are often encountered. Breeding new varieties with multiple resistance and high yield is the fundamental way to solve these problems. But the vast majority of cultivated bananas promoted in production are triploid. They are parthenocarpic and highly sterile females. Conventional cross-breeding methods are very difficult to apply to bananas. Unconventional breeding methods, such as genetic modification, are one way to solve the banana breeding challenge. However, if the explant or callus is directly used as the transformation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 常胜合舒海燕李敬阳许桂莺孙佩光孙威王甲水位明明吴琼明建鸿金志强
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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