Quintuple PCR (polymerase chain reaction) rapid detection method for main pathogenic bacteria in pork

A detection method, a technology of pathogenic bacteria, applied in the field of rapid detection of five-fold PCR, can solve the problems of unreported research, and achieve the effect of good application prospect, fast detection speed and strong specificity

Inactive Publication Date: 2013-09-11
NANJING NORMAL UNIVERSITY +1
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of bacterial 16S rRNA gene as an internal control gene in more than five PCR studies of foodborne pathogens in pork

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quintuple PCR (polymerase chain reaction) rapid detection method for main pathogenic bacteria in pork
  • Quintuple PCR (polymerase chain reaction) rapid detection method for main pathogenic bacteria in pork
  • Quintuple PCR (polymerase chain reaction) rapid detection method for main pathogenic bacteria in pork

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] The following are examples of specific experimental methods.

[0043] 1 Materials and methods

[0044] 1.1 Materials

[0045] 1.1.1 Medium

[0046] Salmonella: HE agar

[0047] Staphylococcus aureus: Baird-Parker agar plates

[0048] Escherichia coli O157:H7: Modified Sorbitol MacConkey (CT-SMAC) Agar

[0049] Listeria monocytogenes: PALCAM agar

[0050] Yersinia enterocolitica: CIN-1 medium

[0051] TSB medium

[0052] All the above media were purchased from Beijing Land Bridge Co., Ltd.

[0053] 1.1.2 Main reagents

[0054] PCR Premix (including TaKaRa Taq, dNTP Mixture, Taq Buffer), DNA Marker (DL2000bp) were purchased from Treasure Bioengineering (Dalian) Co., Ltd.

[0055] Bacterial Genome DNA Extraction Kit (TIANamp Bacteria DNA Kit) was purchased from Beijing Tiangen Biochemical Technology Co., Ltd.

[0056] Agarose (Sunshine, USA) was purchased from Tiangen Biotechnology Company

[0057] Ethidium bromide (EB) was purchased from Tiangen Biotechnology C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a quintuple PCR (polymerase chain reaction) rapid detection method for main pathogenic bacteria in pork. The method comprises the following steps of: extracting total bacteria DNA (deoxyribose nucleic acid), synthesizing the primers represented by the sequences from SEQ ID NO.1 to SEQ ID NO.12; carrying out the quintuple PCR, performing electrophoresis analysis to(on) the PCR product by using 2% agarose, and then judging according to the electrophoresis result. When detecting five food-borne pathogenic bacteria (salmonella, staphylococcus aureus, Listeria monocytogenes, Yersinia enterocolitica and escherichia coli O157:H7) by the method disclosed by the invention, the lowest detectable limits are respectively 142cfu / mL, 51cfu / mL, 9cfu / mL, 33cfu / mL and 670cfu / mL; compared with the traditional bacteria culture and single PCR, the rapid detection method provided by the invention has the characteristics of high detection speed, low cost, high sensitivity and high specificity; and furthermore, the rapid detection method is suitable for the routine safety monitoring of pathogenic bacteria in mass pork and pork products of the food industry during the sales process.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of pathogenic bacteria in food, and relates to a five-fold PCR rapid detection method, in particular to Staphylococcus aureus, Salmonella, Escherichia coli O157:H7, Listeria monocytogenes and enterocolitis in pork Five-fold PCR rapid detection method for five main pathogenic bacteria of Yersinia. Background technique [0002] One of the main problems of global food safety is food-borne diseases, among which the contamination of food-borne pathogenic bacteria is one of the important factors. Pork and its products are rich in nutrients, and are easily infected or polluted by various pathogenic microorganisms in the process of pig breeding, slaughtering, processing, packaging, storage, transportation, sales, etc., causing meat safety problems. In order to ensure the safety of meat products, it is necessary to vigorously strengthen the detection of meat products, especially the rapid detectio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12Q1/10C12R1/445C12R1/42C12R1/19C12R1/01
Inventor 江芸关正萍高峰张林
Owner NANJING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap