Method for extracting alfalfa polysaccharide by use of complex enzyme

Abstract

The invention provides a method for extracting alfalfa polysaccharide by use of complex enzyme (cellulase, papain and pectinase). The extracting temperature of the alfalfa polysaccharide is reduced, the extracting rate of the alfalfa polysaccharide is improved, and the method is suitable for popularization. The method comprises the following steps: determining the test range of factors influencing the extracting rate of the alfalfa polysaccharide through a single-factor test, determining the optimum proportion of the complex enzyme through an orthogonal test, then obtaining the relation between a response value and an influence factor by combing a central complex test with the response surface methodology (RSM), and computing the level of the influence factor when the maximum response value is obtained so as to determine the optimum extracting condition of extracting the alfalfa polysaccharide by complex enzyme, wherein the cellulase is 2.5%, the papain is 2.0%, pectinase is 3.0%, the extracting temperature is 52.7 DEG C, the extracting pH value is 3.87, the extracting solid-to-liquid ratio is 78.91, and the extracting time is 2.73 hours. The extracting rate of alfalfa polysaccharide is more than 5.0% under the condition provided by the invention.

Description

technical field [0001] The invention relates to a method for extracting plant active ingredient polysaccharides, in particular to a method for extracting polysaccharides from alfalfa by using cellulase, papain and pectinase. Background technique [0002] Alfalfa polysaccharides (Alfalfa Polysaccharides) are water-soluble polysaccharides extracted from alfalfa stems and leaves, which have various biological activities. A large number of experiments have proved that within a certain dosage range, alfalfa polysaccharide can promote the growth of livestock and poultry, and improve the immunity, antioxidant capacity and disease resistance of livestock and poultry. In addition, alfalfa polysaccharides also have a certain curative effect on the treatment of high cholesterol, high blood sugar, arteriosclerosis and myocardial infarction, and more importantly, alfalfa polysaccharides have no toxic and side effects on the body. Based on the above advantages, health products and additi...

AI Technical Summary

Problems solved by technology

However, the extraction process of alfalfa polysaccharides is not yet perfect. The current extraction method is mainly hot water alcohol precipitation method. Through continuous heating, the plant cell walls are softened and ruptured, and the polysaccharides overflow

This method not only takes a long time to operate and the extraction rate is low, but also the continuous high temperature heating will destroy the polysaccharide structure and reduce the biological function of the polysaccharide, so the extraction process needs to be improved urgently

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