Tyrosinase inhibitory activity of bunge auriculate root phospholipids and application of bunge auriculate root phospholipid

The technology of Baishouwu phospholipid and tyrosinase diphenolase is applied in the field of tyrosinase inhibitory activity to achieve the effects of good safety, environmental friendliness and prevention of skin tanning

Inactive Publication Date: 2013-09-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have reported that the total phospholipids of Radix Polygoni Multiflori can promote the growth of rabbit ear hair [Wu Bingqin, Fu Jiyuan, Gong Shusheng, etc. Research on the growth of rabbit ear hairs by total phospholipids of Radix Polygoni Multiflori [J]. Chinese Medical Journal, 1987, 2(3) : 29-31.], the patent application with the publication number CN1876003A "The preparation method of the effective part of Baishouwu and its use in the medicine for treating insomnia" discloses the extraction process of Baishouwu phospholipid and Baishouwu polysaccharide and its application in Drugs for treating insomnia and foods for treating or improving insomnia, but there is no report on the activity of phospholipids extracted from Baishouwu medicinal material to inhibit tyrosinase and its related uses

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Preparation of total phospholipids of Radix Polygoni Multiflori: root tubers of Radix Polygoni Multiflori are crushed with a pulverizer, 200 g of Radix Polygoni Multiflori powder is refluxed for 4 hours with 800 mL of Folch reagent (chloroform:methanol is 2:1), the filter residue is repeatedly extracted twice, and the extract is concentrated to dryness, and dissolved with an appropriate amount of chloroform, 5 times petroleum ether to precipitate steroids, and the supernatant obtained by centrifugation was concentrated under reduced pressure and evaporated to a solvent to obtain 2.96 mg of total phospholipids.

Embodiment 2

[0015] Experimental method for inhibition of tyrosinase activity by total phospholipids of Radix Polygoni Multiflori:

[0016] Reagents: Mushroom tyrosinase (EC.1.14.18.1, specific activity 5370U / mg) and levodopa (1-dopa) are both products of Sigma, and hydroquinone (AR grade) is a product of Beijing Zizhu Pharmaceutical Co., Ltd. , other reagents (AR grade) are products of Sinopharm Shanghai Chemical Reagent Company.

[0017] Instrument: AL104 electronic analytical balance, manufactured by Mettler-Toledo (Shanghai) Instrument Co., Ltd.; Muitiskan MK3 multifunctional microplate reader, produced by Shanghai Leibo Analytical Instrument Co., Ltd.

[0018] Tyrosinase was dissolved in phosphate buffer to prepare a 500U / mL solution, subpackaged and frozen, and diluted to 25U / mL with phosphate buffer before the experiment. Before the experiment, an appropriate amount of levodopa was weighed, dissolved in phosphate buffer to prepare a 15 mmol / L solution, shaken, and fully dissolved f...

Embodiment 3

[0026] Take the total phospholipids of Radix Polygoni Multiflori, dissolve it in edible oil at room temperature, and prepare the fruit-shu color-protecting solution with a concentration of 1 mg / mL. Take fresh potatoes, wash them, peel them, cut them into French fries, dry them quickly, put them into the container containing the above color protection solution, be sure to submerge all the fresh-cut potato strips, soak them for 15 minutes, remove them, and fry them directly. It can also be sealed in bags and stored at -20°C to protect French fries from browning.

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Abstract

The invention relates to tyrosinase inhibitory activity of total phospholipid which is extracted and separated from traditional Chinese medicine bunge auriculate root and clarifies a new application of the total phospholipid in the fields of food and daily chemicals. Influence of the total phospholipid on tyrosinase diphenol enzyme activity is detected by virtue of an enzymatic reaction, experimental results prove that the total phospholipid of bunge auriculate root has strong inhibiting effect on mushroom tyrosinase diphenol enzyme activity. The total phospholipids of bunge auriculate root can be used for preparing health care food and cosmetics used for preventing skin from being sunburnt, whitening and removing freckles and can be also used for preparing a fruit and vegetable colour retention agent which can prevent brown stain of food. The application of the total phospholipid of bunge auriculate root based on tyrosinase inhibition activity has the characteristics of nature, safety and no toxicity.

Description

technical field [0001] The invention relates to the tyrosinase inhibitory activity of phospholipid of Radix Polygoni Multiflori and its application. The invention relates to the activity of inhibiting tyrosinase in the total phospholipid extracted and separated from the traditional Chinese medicine Baishouwu, and clarifies its preparation method and its new application in the fields of food and daily chemical industry. Background technique [0002] Tyrosinase is a copper-containing polyphenol oxidase, which is the rate-limiting enzyme in the biosynthesis of melanin, catalyzing the hydroxylation of tyrosine to dopa, the oxidation of dopa to dopaquinone and the 5,6 - Oxidation of dihydroxyindole (DHI) to 5,6-indoloquinone in three steps. Its expression and activity determine the amount and speed of melanin production. Excessive reaction can cause hypermelanin, such as freckles, chloasma, etc. Excessive melanin production is the root cause of chloasma; too weak or lack of rea...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L1/272A61K8/97A61Q19/02A61Q19/00A61Q17/04A23L33/00A23L5/41
Inventor 王晓岚姚文杰
Owner JIANGNAN UNIV
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