Recombinant streptomyces diastatochromogenes with reinforced toyF expression, construction method and uses thereof
A technology for producing Streptomyces chromogenes and amylase, which is applied in the field of genetic engineering to achieve the effect of increasing yield
Active Publication Date: 2014-07-23
CHINA JILIANG UNIV
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Abstract
The invention discloses a recombinant amylase streptomyces chromogenes with enhanced expression of toyF, its construction method and application. It overexpresses the key enzyme of biosynthesis toyocamycin-adenosuccinate lyase encoding gene toyF, and has higher toyocamycin synthesis ability than amylase Streptomyces diastatochromogenes (Streptomyces diastatochromogenes) 1628. The construction process is as follows: 1) Construct the expression vector pIB139-toyF; 2) Use the conjugative transfer method to integrate the expression vector into the chromosome of Streptomyces chromogenes amylase to obtain the engineering bacteria. The promoter permE* on the pIB139 vector was used to promote the expression of the toyF gene, and the vector pIB139-toyF was specifically integrated into the chromosome of Streptomyces diastatochromogenes 1628 by conjugative transfer method, and genetically stable engineering bacteria were obtained. Compared with the original strain, the enzyme activity of adenosine succinate lyase of the recombinant strain was increased by 0.9 times, and the yield of toyocamycin was increased by at least 20.5%.
Application Domain
BacteriaMicroorganism based processes +3
Technology Topic
Molecular biologyAdenylosuccinic acid +14
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PUM
Property | Measurement | Unit |
Relative molecular weight | 53.0 |
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