Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion

A technology that promotes plant growth and mimics Trichoderma koningeni, applied to fungi, etc., to achieve the effects of reducing environmental pollution, uniform seedling emergence, and increasing germination rate

Active Publication Date: 2015-06-17
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on how to scientifically apply the Trichoderma fungus

Method used

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  • Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion
  • Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion
  • Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) bacterial strain prepares the method for Trichoderma inoculum, the steps are as follows:

[0041] 1. Seed preparation

[0042] Using PDA as the activation medium, inoculate the preserved Trichoderma pseudokoningii strain SMF2 on the PDA medium by streaking, culture at 25-28°C for 5-7 days, add appropriate amount of sterile water to the slant seeds to prepare a bacterial suspension These are Shaker Flask Seeds.

[0043] Mix 1 part of bran, 1 part of potato and 5 parts of water, boil for half an hour, filter, add water to the filtrate to the original volume, and then add KH 2 PO 4 0.03~0.05 parts, (NH 4 ) 2 SO 4 0.03~0.05 parts, CaCl 2 0.01-0.02 parts, with natural pH, to prepare seeds for seed jars. After the culture medium is sterilized, inoculate the shake flask seeds according to the weight ratio of 5%, ventilate and stir at 28-30°C, and ferment for 36 hours to obtain the seed pot seeds.

[...

Embodiment 2

[0048] The application of the Trichoderma pseudoconii SMF2 strain in promoting the growth of tobacco, including seedling cultivation application, dipping root application and basal fertilizer application, the steps are as follows:

[0049] (1) Take the number of spores as 2×10 8 / mL of Trichoderma pseudokoningensis SMF2 inoculum, after diluting 100 times, water the seedbed according to the irrigation amount of 10ml / plant, and control the temperature at 15~30°C to prepare the seedbed to be planted;

[0050] (2) Take the number of spores as 2×10 8 / mL of Trichoderma pseudokoningensis SMF2 inoculum, dip the roots at a dose of 0.1g / strain, then transplant it into the seedbed to be planted prepared in step (1), and cultivate it for 50-60 days at 15-30°C , to prepare seedlings to be planted;

[0051] (3) Take the number of spores as 2×10 8 Trichoderma pseudocorningum SMF2 inoculum per mL, mixed evenly with organic fertilizer or sand at a mass ratio of 1:10, to prepare the inoculu...

experiment example

[0054] The experimental example was carried out in Feixian County, Shandong Province, the soil type was cinnamon soil, the previous crop was peanut, and the fertility was medium. Before planting, take a 0-20cm soil sample for testing. The basic physical and chemical properties of the soil are: pH7.33, available nitrogen 108.36mg / kg, available phosphorus 72.88mg / kg, available potassium 331.79mg / kg, and organic matter 24.08g / kg.

[0055] The experiment adopts random block design, the control group is not treated with Trichoderma, and the special seedling fertilizer for tobacco (purchased from Hunan Jinye Zhongwang Technology Co., Ltd.) is applied when transplanting. The treatment group: according to the records in Example 2, when transplanting Apply special seedling fertilizer for tobacco.

[0056] Investigation records

[0057] The investigation of biological traits was carried out at the budding stage, and the plant height, effective leaf number, stem diameter, waist leaf len...

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Abstract

The invention relates to the application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion, and belongs to the technical field of microorganism application. According to the technical scheme, plant growth is promoted according to the following the steps of firstly, preparing a trichoderma pseudokoningii SMF2 microbial agent into a solution which is 100 times the concentration of the trichoderma pseudokoningii SMF2 microbial agent and conducting seed soaking processing; secondly, conducting root dipping processing when trichoderma pseudokoningii SMF2 microbial agent seedlings are transplanted; thirdly, evenly mixing the trichoderma pseudokoningii SMF2 microbial agent with organic fertilizer or fine sandy soil according to the ratio of 1:10 and conducting basal application processing. The trichoderma pseudokoningii SMF2 microbial agent can effectively promote the germination of seeds, the growth of the seedlings and the yield and the quality of crops, and has good application potential in the agricultural production process.

Description

technical field [0001] The invention relates to the application of Trichoderma pseudokoningii SMF2 (Trichoderma pseudokoningii SMF2) bacterial strain in promoting plant growth, and belongs to the technical field of microorganism application. Background technique [0002] The application of chemical fertilizers is of great significance for increasing crop yields and solving food problems. However, due to the one-sided pursuit of yield, excessive application of chemical fertilizers has been caused. Relevant data show that the arable land area is only about 7% of the world's total arable land area, but the amount of chemical fertilizer application exceeds 25% of the world's total amount of chemical fertilizer application, and the amount of chemical fertilizer application per unit of arable land far exceeds the upper limit set by developed countries. Long-term excessive application of chemical fertilizers not only directly affects the normal growth of plants and the quality of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 张玉忠宋晓妍石梅解彬彬陈秀兰董小卫史伟玲巩志廷王丽霞
Owner SHANDONG UNIV
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