Method for synchronously separating effective components in liquorice

A technology for simultaneous separation of active ingredients, applied in medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc. And other issues

Active Publication Date: 2013-10-02
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, patents CN1359905A and CN101766680A use three different solvents to extract three types of components from medicinal residues respectively. There are many impurities, and vice versa; patent CN1803789A extracts the total components in licorice with solvent, and then separates the three types of components by extraction method. Although the operation is simple, if the pH is not adjusted, it is very difficult to use organic solvents alone Difficult to separate glycyrrhizic acid and flavonoids, multiple extractions are required to separate

Method used

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  • Method for synchronously separating effective components in liquorice
  • Method for synchronously separating effective components in liquorice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Take 100g of licorice powder, add 50wt% methanol to reflux extraction 3 times according to the ratio of material to liquid 1:10, 1:8 and 1:8, each time for 1 hour, filter, combine the extracts, recover ethanol under reduced pressure, and the specific gravity of the concentrated solution is 1.15 ( 20°C), add water-saturated n-butanol at a ratio of 1:1, add sodium hydroxide to adjust the pH of the system to 7.0, shake well and extract once, flavonoids are in the n-butanol layer, triterpenoids and sugars are in the water layer, separated the n-butanol layer, recovered the solvent under reduced pressure, and vacuum-dried (60°C) to constant weight to obtain 2.55 grams of crude licorice flavonoids, of which the total flavonoid content of licorice root was 80.75%, and the content of liquiritin was 35.56%. Add water-saturated n-butanol solution at a ratio of 1:1, add hydrochloric acid to adjust the pH of the system to 3.0, shake well and extract once, the triterpenoids are in th...

Embodiment 2

[0065] Take 100g of licorice powder, add 20wt% ethanol to reflux extraction 3 times according to the ratio of material to liquid 1:10, 1:8 and 1:8, each time for 1 hour, filter, combine the extracts, recover ethanol under reduced pressure, and the specific gravity of the concentrated solution is 1.15 ( 20°C), add water-saturated n-butanol at a ratio of 1:1, add sodium hydroxide to adjust the pH of the system to 5.5, shake well and extract once, flavonoids are in the n-butanol layer, triterpenoids and sugars are in the water layer, separated the n-butanol layer, recovered the solvent under reduced pressure, and vacuum-dried (60°C) to constant weight to obtain 3.05 grams of crude licorice flavonoids, of which the total flavonoid content of licorice root was 54.10%, and the content of liquiritin was 22.95%. Add water-saturated n-butanol solution at a ratio of 1:1, add hydrochloric acid to adjust the pH of the system to 4.0, shake well and extract once, the triterpenoids are in the...

Embodiment 3

[0067] Take 100g of licorice powder, add 20wt% ethanol sodium hydroxide mixture (pH10) according to the ratio of material to liquid 1:10, 1:8 and 1:8, reflux extraction 3 times, each time for 1 hour, filter, combine the extracts, and decompress Recover ethanol, the specific gravity of the concentrated solution is 1.15 (20°C), add water-saturated n-butanol at a ratio of 1:1, add sodium hydroxide to adjust the pH of the system to 7.0, shake well and extract once, the flavonoids are in the n-butanol layer, three The terpenoids and carbohydrates were in the water layer, and the n-butanol layer was separated. After the solvent was recovered under reduced pressure, it was vacuum-dried (60°C) to constant weight to obtain 2.70 g of crude licorice flavonoids, of which the total flavonoid content of licorice was 85.42%. The content of glycosides is 37.55%; add water-saturated n-butanol solution to the water layer at a ratio of 1:1, add hydrochloric acid to adjust the system 3.0, shake we...

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Abstract

The invention relates to a method for synchronously separating effective components in liquorice, and belongs to the fields of medicines, foods and chemical industry. According to the method, by utilizing the chemical structure characteristics of flavonoid components, triterpene components and polysaccharide components in the liquorice as well as the dissolution characteristics of the flavonoid components, the triterpene components and the polysaccharide components in different solvents and under different pH values, the three types of the main active components, namely, the flavonoid components, the triterpene components and the polysaccharide components in the liquorice are separated synchronously. After an organic reagent is added into an aqueous solution of a licorice extract, by regulating the pH value of the system to be as 7.0, the flavonoid components of the liquorice are contained in an organic layer while the triterpene components and the polysaccharide components of the liquorice are contained in an aqueous layer; then the aqueous layer therein is separated; after the organic reagent is added, by regulating the pH value of the system to be as 3.0, the flavonoid components are contained in the organic layer while the polysaccharide components are contained in the aqueous layer. As the method is used, the loss of the other effective components, caused by single or intermittent extraction and separation, is avoided; the extraction rate of the effective components as well as the yield and purity of separating the three types of the components are improved greatly after a basic solvent is extracted. In addition, the reagent consumption is reduced. The method is simple and easy to implement, low in energy consumption and low in cost. Thus, the method is more suitable for industrial production.

Description

technical field [0001] The invention relates to a method for synchronously separating active components of licorice from licorice extract, belonging to the fields of medicine, food and chemical industry. Background technique [0002] Licorice is a perennial herb of the genus Glycyrrhizae in the leguminous family. It is a medicinal root with a sweet and flat nature. Licorice has a long history of being used as medicine. As early as more than 2,000 years ago, "Shen Nong's Materia Medica" listed it as the best medicine. Tao Hongjing, a medical scientist in the Southern Dynasties, respected licorice as the "elder of the country" and said: "This herb is the king of all medicines, and there are few people who don't use it in prescriptions." Licorice has the reputation of "ten directions and nine herbs" and is widely used in clinical medicine. The formula can detoxify 1,200 grasses and trees, and reconcile all medicines. Licorice extract is also widely used in food, chemical indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/484A61K8/97A23L1/221C08B37/00A23L27/10A61K8/9789
Inventor 刘桂艳王栋汪竹高自强金炜锟
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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