Method for extracting resveratrol from grape peel residue with enzymatic method

Abstract

The invention aims to provide a method for extracting resveratrol from grape peel residue with an enzymatic method, wherein the method is simple, high in extraction efficiency and low in production cost. The method for extracting the resveratrol from the grape peel residue with the enzymatic method comprises the following technical steps: (1) drying grape peel residue: air-drying and removing remaining water of grape peel residue after wine is recycled through natural wind until the moisture content is smaller than 4%; (2) crashing: crashing the grape peel residue after air-drying into 60-80 meshes; (3) weighing the grape peel residue crashed into 60-80 meshes, adding redistillation water and cellulase for enzymolysis, adding acetic ether for digestion after enzymolysis, and performing digestion one to five times repeatedly; (4) after mixing concentrate through repeated digestion, putting in a vacuum drying box at the temperature of 40-60 DEG C, and drying to obtain resveratrol crude powder; and (5) purifying the resveratrol so as to obtain resveratrol with the purity of more than 95% finally.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of active ingredients of natural products, and in particular relates to a method for extracting resveratrol from grape pomace by using an enzymatic method. Background technique [0002] Resveratrol (resveratrol: trans-3,5,4'-trihydroxystilbene) is a stilbenes compound (stilbenes), which exists in some medicinal plants and edible plants, such as cassia, veratrum, Knotweed, grapes, peanuts. [0003] Resveratrol (Res) is not only a chemopreventive agent for tumor diseases, but also a chemopreventive agent for reducing platelet aggregation, preventing and treating atherosclerosis, cardiovascular and cerebrovascular diseases, inhibiting abnormal aggregation of platelets, and preventing myocardial infarction, cerebral palsy, etc. Embolization has a protective effect on the hypoxic heart, effectively recovers the decrease in cardiac output caused by burns or hemorrhagic shock, and can ...

AI Technical Summary

Problems solved by technology

[0006] At present, the methods for extracting resveratrol (Res) from grape pomace mainly include organic solvent extraction, enzymatic extraction, microwave extraction, supercritical CO: extraction and ultrasonic extraction, etc. The main problems in the above methods are as follows : 1. The solvent extraction method mainly has a lot of organic solvent consumption, long extraction process, high production cost, residual solvent in the obtained product, and solvent leakage during the extraction process, resulting in poor extraction environment and environmental pollution. , if the leakage of the solvent is controlled, it will inevitably cause a series of problems such as complex production equipment, high investment and operating costs, and increased costs; 2. The supercritical CO2 extraction method is mainly due to high production costs and relatively high requirements for operating conditions; 3. The disadvantage of the microwave extraction method is that the selection of the extraction medium has a great influence on the product components, resulting in the loss of some active ingredients

Images