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Medical instrument for remotely activating nerve cells

A neuron and remote technology, applied in the field of medical instruments, can solve the problems of limited tissue penetration of light, permanent implantation of optical fibers, and limited application, and achieve strong tissue penetration, high cell specificity, and high application value. Effect

Inactive Publication Date: 2013-10-09
李晓明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, optogenetics has shortcomings such as limited tissue penetration of light, the need for permanent implantation of optical fibers, and tissue damage, which limits its clinical application.

Method used

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  • Medical instrument for remotely activating nerve cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: The formation of instrument system

[0013] like figure 1 As shown, the instrument system: signal generator, coil, magnetic field detector, we use the signal generator to generate DC and AC signals of different waveforms for different degrees of magnetic field control, electromagnetic waves will be generated during the propagation of the magnetic field, and The electromagnetic waves we use are within the wavelength range of radio waves. Radio waves widely exist in our daily life and have various applications, including wireless data networks, various mobile communications, and radio broadcasting. Because the radio can penetrate deep tissue, less energy is lost during the penetration process, and there is little damage to the tissue. Therefore, the system of the present invention can use radio to activate neurons remotely and non-invasively.

Embodiment 2

[0014] Embodiment 2: Construction of vector and packaging of virus

[0015] The virus expressing iron nanoparticles and temperature-sensitive channels of the present invention is prepared by the following method: In order to express iron nanoparticles that can be heated by radio, we use the expression iron ion storage protein to capture iron ions in cells, which can be expressed in neurons Paramagnetic iron nanoparticles are naturally formed in . The temperature-sensitive channel TRPV1 is expressed in neurons expressing ferrofusion protein at the same time, and the radio diffuse heating of the whole brain will cause the temperature of iron nanoparticles to rise, thereby increasing the temperature of neurons expressing ferrofusion protein, which in turn will cause the TRPV1 channel. Opening and activation of neurons. We will use viruses to achieve co-expression of ferrofusion protein and TRPV1 in neurons. The virus is a virus AAVHSTF, Virus AAVHTF, Virus AAVMTF, Virus ...

Embodiment 3

[0018] Example 3: Detection of TRPV1 channel opening caused by radio wave heating of iron ion nanoparticles.

[0019] First, verify the effectiveness of the system in HEK293T cells cultured in vitro, introduce the target gene fragments by plasmid transfection or virus transfection, observe the cell growth status, and then use the method of calcium imaging to give the successfully transfected cells the The cells expressing green fluorescent protein are heated by radio waves, and the signal generator generates a sine wave signal and passes through the amplifier, which is applied to the electromagnet coil to generate a magnetic field with a strength of 5 mT. This setup was used to successfully heat iron ion nanomaterials to open TRPV1 channels in vitro and in vivo. Calcium influx can be detected when the temperature reaches the TRPV1 opening threshold of 42 °C.

[0020] Then, in the neurons cultured in vitro, the target fragment was introduced into the neurons by plasmid transfe...

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Abstract

The invention discloses a medical instrument for remotely activating nerve cells. According to the medical instrument, a virus is used for infecting the nerve cells so as to enable the virus to express iron nano particles and a temperature sensitive channel and remotely give radio waves to activate the nerve cells. The whole medical instrument system comprises a signal generator, a coil, a magnetic field detector and the virus provided with a target fragment. An electrode or an optical fiber is not required to be implanted into the virus which expresses the iron nano particles and the temperature sensitive channel, damage to animal tissues does not exist, tissue penetration is strong, dispersive stimulation within the range of the whole brain can be carried out, time accuracy and space accuracy are high, real-time control over animals which soberly and freely move can be carried out, cell specificity is high, and therefore the medial instrument has higher application value in research for brain functions and treatment of clinical neuropsychiatric disorders.

Description

technical field [0001] The present invention relates to a medical instrument, in particular to a medical instrument for activating neurons by using a virus to infect neurons to express iron nanoparticles and temperature-sensitive channels, and remotely giving radio waves to activate neurons and its application. Background technique [0002] The nervous system is the "command" of the organism to collect, analyze information, and issue instructions. The central nervous system mediates the generation of advanced functions such as emotion, attention, learning, and memory, and participates in the generation of sensations and the initiation of voluntary movements. Neurons are the functional units of the nervous system that perform multiple functions. There are about 100 billion neurons in the human brain. A large number of neurons form synaptic connections to exchange information and form complex neural loops at different levels. roads and neural networks. When studying the comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61N5/00A61K41/00A61P25/00
Inventor 李晓明
Owner 李晓明