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Separating and purifying method of lamiophlomis rotata total flavonoid

A separation and purification, unique technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve problems such as low recovery rate, low product purity, and restrictions on the development of MAR. Achieve good adsorption and separation effect

Inactive Publication Date: 2013-10-23
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since different MARs have different structural parameters such as polarity, functional group, pore size, pore volume, and specific surface area, and a certain MAR has different degrees of adsorption on multiple components, therefore, for some substances, the structure is relatively similar. For the separation of components, it is often difficult to achieve a good separation effect by using a single type of MAR. Usually, the recovery rate of the product with high purity after separation is low, and the product with high recovery rate is low in purity, which limits the application of MAR to a certain extent. develop well

Method used

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  • Separating and purifying method of lamiophlomis rotata total flavonoid
  • Separating and purifying method of lamiophlomis rotata total flavonoid
  • Separating and purifying method of lamiophlomis rotata total flavonoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Weigh 10.000g of Duyiwei dried leaves and place in a 250mL round bottom flask filled with 100mL of 25% ethanol solution. In the ultrasonic device for 120min, the ultrasonic extraction experiment of the total flavonoids of unifoliate was carried out. Repeat three times, combine the extracts obtained each time and filter them with nylon mesh, discard the residue, collect the sample solution, place it in a petri dish that has been dried to constant weight, evaporate it to dryness in a water bath, and dry it in vacuum at 65°C for 10 hours, scrape Take the net paste, mix well and set aside.

[0056] Weigh MAR XDA8 with a dry weight of about 1.0g, put it into a tea bag with a size of 2×6cm made of a 200-mesh filter, then put the tea bag into a 100mL Erlenmeyer flask, add 50mL of ethanol to soak for 24h, Recover soaking solution. Then add an appropriate amount of ethanol to wash until 3 times the volume of distilled water is added and no white turbidity appears. Add an appr...

Embodiment 2

[0058] Weigh 10.000g of Duyiwei dried leaves and place in a 250mL round bottom flask filled with 100mL of 25% ethanol solution. In the ultrasonic device for 120min, the ultrasonic extraction experiment of the total flavonoids of unifoliate was carried out. Repeat three times, combine the extracts obtained each time and filter them with nylon mesh, discard the residue, collect the sample solution, place it in a petri dish that has been dried to constant weight, evaporate it to dryness in a water bath, and dry it in vacuum at 65°C for 10 hours, scrape Take the net paste, mix well and set aside.

[0059] Weigh MAR DM130 with a dry weight of about 1.0g, put it into a tea bag with a size of 2×6cm made of a 200-mesh filter, then put the tea bag into a 100mL Erlenmeyer flask, add 50mL of ethanol to soak for 24h, Recover soaking solution. Then add an appropriate amount of ethanol to wash until 3 times the volume of distilled water is added and no white turbidity appears. Add an app...

Embodiment 3

[0061] Weigh 10.000g of Duyiwei dried leaves and place in a 250mL round bottom flask filled with 100mL of 25% ethanol solution. In the ultrasonic device for 120min, the ultrasonic extraction experiment of the total flavonoids of unifoliate was carried out. Repeat three times, combine the extracts obtained each time and filter them with nylon mesh, discard the residue, collect the sample solution, place it in a petri dish that has been dried to constant weight, evaporate it to dryness in a water bath, and dry it in vacuum at 65°C for 10 hours, scrape Take the net paste, mix well and set aside.

[0062] Weigh the MAR mixed bed XDA8 and DM130 with a dry weight of about 1.0g, put them into a tea bag with a size of 2×6cm made of a 200-mesh filter, then put the tea bag into a 100mL Erlenmeyer flask, add 50mL of ethanol After soaking for 24 hours, recover the soaking solution. Then add an appropriate amount of ethanol to wash until 3 times the volume of distilled water is added and...

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Abstract

The invention discloses a separating and purifying method of lamiophlomis rotata total flavonoid. The method comprises the following steps of: (a) crushing root lamiophlomis rotata; (2) putting powdery lamiophlomis rotata into ethanol water according to the ratio that the solid-liquid ratio g / mL of the lamiophlomis rotata to the ethanol water is 1:(5-15), and extracting by ultrasonic sound, wherein the ultrasonic power is 80-100 W; the ultrasonic temperature is 30-60 DEG C; the extraction time is 1-4 hours; and the extraction times is 1-4; (3) filtering an extracting solution, drying in a vacuum condition, and recovering extractum; (4) preparing into a 0.5-2 mg / mL solution from obtained lamiophlomis rotata extractum, and adsorbing by two macroporous adsorption resin mixed beds; (5) washing the adsorbed macroporous adsorption resin mixed beds by distilled water, and then carrying out desorption by adding the ethanol water; and (6) evaporating, concentrating and drying the ethanol desorption solution to obtain the lamiophlomis rotata total flavonoid.

Description

technical field [0001] The invention relates to a method for separating and purifying active ingredients of Chinese herbal medicines. Background technique [0002] Duyiwei, also known as "Daba" in Tibetan, scientific name Lamiophlmis rotat (Benth.) Kudo, is a stemless perennial herb belonging to the genus Lamiophlmis rotat (Benth.) Kudo. [0003] The extraction and separation of active components of Chinese herbal medicine is very important in the process of modernization and internationalization of Chinese medicine. Through the screening and analysis of effective parts, the drug action basis of active components of Chinese medicine can be clarified, which is convenient for the development and innovation of new Chinese medicines. The advantages of MAR in the separation and purification of active ingredients of Chinese herbal medicines are mainly manifested in the following two aspects: first, the active ingredients of many single Chinese herbal medicines have been successful...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61K125/00
Inventor 陈振斌刘晓娇李洁邓伟溪
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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