In vitro culture and vitality determination method for water lily pollen

A technology for viability measurement and in vitro culture, applied in biochemical equipment and methods, microbe measurement/inspection, plant cells, etc., can solve the problems of in vitro culture medium and culture conditions for unseen water lily pollen, and achieve simple measurement methods , high practical application value, the effect of improving softness and permeability

Inactive Publication Date: 2013-11-20
ZHENJIANG SUIHAN AGRI
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Problems solved by technology

At present, there is no report on the culture medium and cu

Method used

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the embodiment of water lily variety "Peter".

[0020] This embodiment includes the following steps:

[0021] 1) Preparation of liquid medium

[0022] Prepare liquid medium with the following components: 300 g / L sucrose, 10 mg / L boric acid, 350 mg / L magnesium sulfate, 900 mg / L potassium nitrate, 800 mg / L calcium chloride and 100 g / L Phytohormone PEG1500, the rest is the honey liquid on the stigma of water lily.

[0023] 2) Water lily pollen collection

[0024] When "Peter" blooms in late July, collect water lily pollen in full bloom around 10:00 in the morning, put the water lily pollen into a centrifuge tube, and keep it away from light for later use.

[0025] 3) Water lily pollen cultivation

[0026] Drop the liquid medium on the glass slide, dip a small amount of water lily pollen into the liquid medium with a brush, without covering the slide, place the slide in a petri dish lined wit...

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Abstract

The invention discloses an in vitro culture and vitality determination method for water lily pollen. The method comprises the following steps: 1) a preparing a mixed liquid medium containing cane sugar, boric acid, potassium nitrate, calcium chloride, polyethylene glycol, hormone and water lily flower stigma disc; 2) acquiring pollen of blooming water lily last ten-days of July in blooming period; 3) dripping the liquid medium on a piece of slide glass, dipping a small amount of pollen into the liquid medium and culturing the pollen at 25 DEG C in a dark environment; and 4) culturing the slide glass for 6 h and observing germination status under an optical microscope, thereby determining the vitality of water lily pollen. The in vitro culture method for water lily in vitro provided by the present invention is scientific and reasonable; the vitality determination method for water lily pollen is simple, has strong operationality and reliable data, and can completely realize quantitative determination. Therefore, the invention has high actual application value for raising breeding efficiency of water lily.

Description

technical field [0001] The invention relates to a method for culturing pollen in vitro, in particular to a method for culturing in vitro, and also relates to a method for measuring the vigor of water lily pollen, which belongs to the technical field of plant cross breeding. Background technique [0002] Nymphaea is a genus of Nymphaeaceae ( Nymphaea ) is a general term for plants. It is deeply loved by people and widely used in garden waterscapes for its rich flower colors, holy implication, and strong adsorption capacity of stems and leaves to nutrient and harmful substances in water. [0003] The determination of the pollen viability of different varieties of water lilies can distinguish the survival rate of the tested varieties of water lily pollen, which has important theoretical and practical significance for guiding water lily breeding. There are many ways to measure the vitality of pollen. Among them, the in vitro germination of pollen can visually observe the po...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12Q1/02
Inventor 孙春青潘跃平戴忠良马志虎孙国胜
Owner ZHENJIANG SUIHAN AGRI
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