Method for extracting sulforaphane from broccoli

Abstract

The invention relates to a method for extracting sulforaphane from broccoli. The method comprises the following steps: (1) drying edible flowering branches of fresh broccoli to constant weight and crushing to obtain broccoli dry powder; (2) carrying out vacuum filtration on newly harvested crushed mustard seeds to remove impurities to obtain a crude enzyme; (3) adding the crude enzyme into the broccoli dry powder, uniformly stirring, and carrying out enzymolysis and ultrasonic vibration to extract to obtain extracting liquor which is screened and deslagged to obtain a filtrate; carrying out vacuum filtration on the filtrate to obtain sulforaphane coarse extracting liquor; (4) vacuum concentrating the sulforaphane coarse extracting liquor to obtain sulforaphane coarse extracting aqueous liquor; (5) adding ethyl acetate into the sulforaphane coarse extracting aqueous liquor to extract to obtain an ethyl acetate phase, carrying out vacuum concentration on the ethyl acetate phase to get paste, and then eluting the paste and further vacuum-concentrating to obtain a paste sulforaphane coarse extract; (6) freezing and drying the paste sulforaphane coarse extract to constant weight to obtain the sulforaphane coarse product which is 1-5% in purity. The method provided by the invention is simple, efficient and low in cost.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients in natural products, in particular to a method for extracting sulforaphane from broccoli. Background technique [0002] For a long time, people have regarded vegetables as an important source of vitamins and trace elements for the human body. In recent years, scholars at home and abroad have found that some compounds in cruciferous vegetables have anticancer activity, especially vegetables rich in carotene, such as: carrots, broccoli, broccoli and spinach. Experiments have shown that broccoli ( broccoli ) is rich in anti-cancer substance sulforaphane, which can reduce the formation of tumors in the chest of mice, prevent the activity of carcinogens, and prevent the occurrence of leukemia. [0003] Sulforaphane is an isothiocyanate derivative produced by glucoraphanin (which is a glucosinolate, a type of glucosinolate) by myrosinase hydrolysis or acid hydrolysis, with a ...

AI Technical Summary

Problems solved by technology

At present, the main source of sulforaphane is extracted from broccoli seeds, which costs more than 4,000 yuan per kilogram of seeds, and the cost of raw materials is too high, which is not conducive to industrial production

There have been researches on the artificial synthesis of sulforaphane, but it cannot be mass-produced at present. Secondly, the synthesized sulforaphane is a racemate, which has weakened functions and has certain toxic and side effects.