Method for extracting sulforaphane from broccoli

A technology of sulforaphane and broccoli, applied in the direction of organic chemistry, fermentation, etc., can solve the problems of unfavorable industrialized production, high cost of raw materials, toxic and side effects, etc., and achieve the effects of low cost, large base area and less pollution sources.

Inactive Publication Date: 2013-12-11
GANSU AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main source of sulforaphane is extracted from broccoli seeds, which costs more than 4,000 yuan per kilogram of seeds, and the cost of raw materials is too high, which is not conducive to industrial production
There

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 A method for extracting sulforaphane from broccoli, comprising the following steps:

[0023] (1) Dehydrate the edible part of fresh broccoli at 75°C for 1.5 hours, then dry at 55°C to constant weight to obtain broccoli dry matter; grind the broccoli dry matter to 40 mesh to obtain broccoli dry powder.

[0024] (2) Crush the newly harvested mustard seeds to 40 mesh to obtain mustard seed powder; add distilled water to the mustard seed powder, vibrate ultrasonically for 30 minutes at a power of 500W and a temperature of 25°C, and then vacuum filter to remove impurities to obtain crude enzyme liquid.

[0025] Among them: the ratio of mustard powder to distilled water is 1 g: 40 mL.

[0026] (3) Add crude enzyme solution to broccoli dry powder, stir evenly, enzymatically hydrolyze at 35°C for 0.5h, then add ethanol with a mass concentration of 95%, extract by ultrasonic vibration for 60min under the conditions of power of 500W and temperature of 25°C, and th...

Embodiment 2

[0035] Example 2 A method for extracting sulforaphane from broccoli, comprising the following steps:

[0036] (1) Dehydrate the edible part of fresh broccoli at 85°C for 0.5 hours, and then dry at 65°C to constant weight to obtain broccoli dry matter; grind the broccoli dry matter to 60 mesh to obtain broccoli dry powder.

[0037] (2) Crush the newly harvested mustard seeds to 60 mesh to obtain mustard seed powder; add distilled water to the mustard seed powder, vibrate ultrasonically for 10 minutes at a power of 800W and a temperature of 35°C, and then vacuum filter to remove impurities to obtain crude enzyme liquid.

[0038] Among them: the ratio of mustard powder to distilled water is 1 g: 60 mL.

[0039] (3) Add crude enzyme solution to broccoli dry powder, stir evenly, enzymatically hydrolyze at 35°C for 1.5h, then add ethanol with a mass concentration of 95%, extract by ultrasonic vibration for 10min at a power of 800W and a temperature of 35°C, and then Stand sti...

Embodiment 3

[0048] Example 3 A method for extracting sulforaphane from broccoli, comprising the following steps:

[0049] (1) The edible part of fresh broccoli is dehydrated at 80°C for 1.0 hour, and then dried at 60°C to constant weight to obtain broccoli dry matter; the broccoli dry matter is crushed to 50 mesh to obtain broccoli dry powder.

[0050](2) Crush the newly harvested mustard seeds to 50 mesh to obtain mustard seed powder; add distilled water to the mustard seed powder, vibrate ultrasonically for 20 minutes at a power of 650W and a temperature of 30°C, and then vacuum filter to remove impurities to obtain crude enzyme liquid.

[0051] Among them: the ratio of mustard powder to distilled water is 1 g: 50 mL.

[0052] (3) Add crude enzyme solution to broccoli dry powder, stir evenly, enzymatically hydrolyze at 35°C for 1.0h, then add ethanol with a mass concentration of 95%, extract by ultrasonic vibration for 35min at a power of 56500W and a temperature of 30°C, and then...

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PUM

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Abstract

The invention relates to a method for extracting sulforaphane from broccoli. The method comprises the following steps: (1) drying edible flowering branches of fresh broccoli to constant weight and crushing to obtain broccoli dry powder; (2) carrying out vacuum filtration on newly harvested crushed mustard seeds to remove impurities to obtain a crude enzyme; (3) adding the crude enzyme into the broccoli dry powder, uniformly stirring, and carrying out enzymolysis and ultrasonic vibration to extract to obtain extracting liquor which is screened and deslagged to obtain a filtrate; carrying out vacuum filtration on the filtrate to obtain sulforaphane coarse extracting liquor; (4) vacuum concentrating the sulforaphane coarse extracting liquor to obtain sulforaphane coarse extracting aqueous liquor; (5) adding ethyl acetate into the sulforaphane coarse extracting aqueous liquor to extract to obtain an ethyl acetate phase, carrying out vacuum concentration on the ethyl acetate phase to get paste, and then eluting the paste and further vacuum-concentrating to obtain a paste sulforaphane coarse extract; (6) freezing and drying the paste sulforaphane coarse extract to constant weight to obtain the sulforaphane coarse product which is 1-5% in purity. The method provided by the invention is simple, efficient and low in cost.

Description

technical field [0001] The invention relates to a method for extracting and purifying active ingredients in natural products, in particular to a method for extracting sulforaphane from broccoli. Background technique [0002] For a long time, people have regarded vegetables as an important source of vitamins and trace elements for the human body. In recent years, scholars at home and abroad have found that some compounds in cruciferous vegetables have anticancer activity, especially vegetables rich in carotene, such as: carrots, broccoli, broccoli and spinach. Experiments have shown that broccoli ( broccoli ) is rich in anti-cancer substance sulforaphane, which can reduce the formation of tumors in the chest of mice, prevent the activity of carcinogens, and prevent the occurrence of leukemia. [0003] Sulforaphane is an isothiocyanate derivative produced by glucoraphanin (which is a glucosinolate, a type of glucosinolate) by myrosinase hydrolysis or acid hydrolysis, with a ...

Claims

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Application Information

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IPC IPC(8): C12P13/00C07C331/22
Inventor 李胜唐斌周文政马绍英张品南刘会杰赵生琴时振振苏李维
Owner GANSU AGRI UNIV
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