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Method for fermentation production of natural beta-carotene by adding multiple fermentation accelerants

A technology of fermentation accelerator and carotene, which is applied in the field of production of β-carotene, can solve the problems such as the reduction of the multiple of fermentation increase, and achieve the effect of increasing fermentation output and enhancing the combination effect

Active Publication Date: 2013-12-11
SHANGHAI RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And when the production of β-carotene in comparison is increased to 0.305g / L (adding Mg +2 , and β-ionone), the fermentation yield was only increased to 0.342g / L, and the increase was far lower than that of the strains with low yield compared to other strains. However, no combination studies of more fermentation accelerators were carried out to further improve the fermentation yield. Especially when the comparative yield is further improved, that is, when a strain with high comparative yield is selected, the multiple of its fermentation increase will be further reduced

Method used

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  • Method for fermentation production of natural beta-carotene by adding multiple fermentation accelerants
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Embodiment 1

[0022] Blakeslea Trispora (Blakeslea Trispora), fermentation medium consists of starch 8% (weight), meal meal 1.6% (weight), vegetable oil 6.6% (v / v), KH 2 PO 4 0.06% (weight), MnSO 4 ·H 2 0.03% (weight), adjust the pH to be 7.0, add positive and negative bacteria mixed seed liquid after the fermented medium sterilized for preparation, after 50 hours of fermentation, add the replenishing liquid that contains fermentation accelerator in fermented liquid, its Composed of Mg 2+ 0.25mmol / L, hydrogen peroxide 30μmol / L, Span201.0g / L, decane 2% (v / v), sodium formate 0.8mmol / L, sodium citrate 0.5g / L, at 27°C on a shaking table with a rotation speed of 230rpm The fermented liquid was obtained after 120 hours of fermentation and cultivation, and the analysis result showed that the content of β-carotene in the fermented liquid was 2.27g / L.

Embodiment 2

[0028] The method for fermenting and producing natural beta-carotene by adding various fermentation accelerators, the method comprises the steps of slant culture, seed culture and fermentation culture, specifically comprising the following steps:

[0029] (1) There are many kinds of microorganisms that can produce natural β-carotene by fermentation, among which Blakeslea Trispora has the best performance, and is internationally recognized as a good candidate for industrialized production of natural β-carotene. strain. Belonging to the fungal door Fungi (Fungi), Mucorales, Thaxteraceae, Brucella genus, and B.Trispora Thaxter (B.Trispora Thaxter), the method of the present invention is to ferment and produce natural β- The bacterial strain of carotene is Blakeslea Trispora (Blakeslea Trispora), which has positive and negative strains, and is preserved in the bacterial strain collection of Shanghai Research Institute of Chemical Industry.

[0030] The filamentous fungus Blakesle...

Embodiment 3

[0034] The method for fermenting and producing natural beta-carotene by adding various fermentation accelerators, the method comprises the steps of slant culture, seed culture and fermentation culture, specifically comprising the following steps:

[0035](1) inoculate the filamentous fungus Blakeslea Trispora (Blakeslea Trispora) on the slant medium, and the fungus Blakeslea Trispora is divided into positive and negative strains, and the adopted slant medium matrix is ​​peeled potatoes, control The mass ratio of potatoes to water is 20:90, after boiling for 20 minutes, filter, add glucose, peptone, agar, and vitamin B at a mass ratio of 2:1:3 1 5ppm, and sterilized, when cultivating on a slant, control the temperature at 27°C and cultivate for 72 hours;

[0036] (2) Inoculate the positive and negative strains of B. trispora through slant-cultured Blakeslea trispora into the seed culture medium in a ratio of 1:5, and the seed culture medium used is 10wt% of meal meal, 7wt% of c...

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Abstract

The invention relates to a method for the fermentation production of natural beta-carotene by adding multiple fermentation accelerants. The method comprises the working procedures of slant culture, seed culture and fermentation culture, wherein during fermentation culture, the fermentation culture production of the natural beta-carotene is promoted by adding the fermentation accelerants. Compared with the prior art, the method has the advantages that the multiple fermentation accelerants obtained by test comparison are added to a fermentation medium, comprise a surface active agent, alkane, part of metabolites in tricarboxylic acid cycle, metal ions and oxygen stress effect matters, are expected to stimulate breath and growth by changing the permeability of cell membranes and increase the solubility of oxygen so as to meet the aerobic fermentation requirements of blakeslea trispora.

Description

technical field [0001] The invention relates to a method for producing beta-carotene, in particular to a method for fermenting and producing natural beta-carotene by adding various fermentation accelerators. Background technique [0002] In the prior art, Chinese patent CN1331343A (application number 00116698.0, application date June 22, 2000) discloses a method for producing natural beta-carotene by fermentation, which is to combine the positive and negative The strain was inoculated and the filamentous fungus was fermented to produce β-carotene in a stirred fermenter. The improvement of the stirring blade improved the mass transfer performance of the fermented liquid, which was more suitable for the growth of the bacteria. The fermentation level could reach 2.0g / L, without Work on the use of fermentation accelerators to increase fermentation yields. [0003] <Journal of Wuxi University of Light Industry> 2003 V.22, No.3 reported "Mg 2+ Effects on the synthesis of β...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 徐大刚李泓全董宏伟徐静安杨晔陈慧芳尹金凤蔡扬孙建春喻静兰吴欣森
Owner SHANGHAI RES INST OF CHEM IND