Preparation method of corn peptide glycosylation product nano particles embedding lipid-soluble vitamins

A technology of fat-soluble vitamins and nanoparticles, applied in food science, food forming, application, etc., can solve the problems of low bioavailability, achieve the effects of improving solubility, good transparency, and improving stability

Active Publication Date: 2013-12-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the deficiencies of the above-mentioned prior art, the object of the present invention is to provide a preparation method of corn peptide glycosylation product nanoparticles em

Method used

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  • Preparation method of corn peptide glycosylation product nano particles embedding lipid-soluble vitamins
  • Preparation method of corn peptide glycosylation product nano particles embedding lipid-soluble vitamins
  • Preparation method of corn peptide glycosylation product nano particles embedding lipid-soluble vitamins

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Dissolve 2g of corn peptide and 2g of maltodextrin in 20mL of 20mM, pH7.5 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, stir at 500rpm for 2h; / L sodium hydroxide to adjust the pH value to 7.5, stirred at 500rpm for 2h, and then freeze-dried; put the dry powder in a closed desiccator, and reacted for 4 days at a relative humidity of 79% and a temperature of 60°C; the obtained solid was dissolved in 40mL to In ionized water, stir at 500rpm for 2h, centrifuge at 10000rpm for 15min, take the supernatant, freeze-dry, and store the dried powder in a refrigerator at 4°C to obtain corn peptide‐maltodextrin glycosylation products, wherein the mass ratio of corn peptide to maltodextrin is 1 :1.

[0029] Dissolve 80mg of corn peptide‐maltodextrin glycosylation product in 19.2mL of deionized water, stir at 500rpm for 1, adjust pH5 with 0.1mol / L hydrochloric acid; dissolve 20mg of vitamin E in 10mL of absolute ethanol, stir at 500rpm for 30min; take 0.8 ...

Embodiment 2

[0043] Dissolve 1 g of corn peptide and 1 g of maltodextrin in 20 mL of 10 mM, pH 7.5 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution, stir at 500 rpm for 1 h; / L sodium hydroxide to adjust the pH to 7.5, stirred at 500rpm for 2h, and then freeze-dried; put the dry powder in a closed desiccator, and reacted for 5 days at a relative humidity of 79% and a temperature of 65°C; the obtained solid was dissolved in 20mL of deionized Stir in water at 500rpm for 2h, centrifuge at 10000rpm for 15min, take the supernatant and freeze-dry it, and store the dried powder in a refrigerator at 4°C to obtain corn peptide-maltodextrin glycosylation products, wherein the mass ratio of corn peptide to maltodextrin is 2:1.

[0044] Dissolve 120mg of corn peptide-maltodextrin glycosylation product in 19.0mL of deionized water, stir at 500rpm for 2h, adjust pH6 with 0.1mol / L sodium hydroxide; dissolve 50mg of vitamin D in 10mL of absolute ethanol, stir at 500rpm for 40min; Ta...

Embodiment 3

[0052] Dissolve 2g of corn peptide and 2g of maltodextrin in 20mL of 10mM, pH7.5 sodium dihydrogen phosphate-disodium hydrogen phosphate buffer, stir at 500rpm for 2h; / L sodium hydroxide to adjust the pH to 7.5, stirred at 500rpm for 2h, and then freeze-dried; put the dry powder in a closed desiccator, and reacted for 6 days at a relative humidity of 79% and a temperature of 55°C; the obtained solid was dissolved in 40mL of deionized In water, stir at 500rpm for 2h, centrifuge at 10000rpm for 15min, take the supernatant and freeze-dry, and store the dried powder in a refrigerator at 4°C to obtain corn peptide-maltodextrin glycosylation products, wherein the mass ratio of corn peptide to maltodextrin is 1:2.

[0053] Dissolve 60mg of corn peptide‐maltodextrin glycosylation product in 19.5mL of deionized water, stir at 500rpm for 1h, adjust pH5 with 0.1mol / L hydrochloric acid; dissolve 40mg of vitamin E in 10mL of absolute ethanol, stir at 500rpm for 35min; take 0.5 Add mL of v...

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Abstract

The invention discloses a preparation method of corn peptide glycosylation product nano particles embedding lipid-soluble vitamins. The method comprises the following steps: firstly dissolving a corn peptide and a polysaccharide into a phosphate buffer respectively, mixing the corn peptide solution with the polysaccharide solution, then freeze-drying the mixture, and putting the obtained dry powder in a closed dryer to carry out dry hot reaction at 55-65 DEG C for 4-7 days; obtaining a corn peptide glycosylation product; then adding the corn peptide glycosylation product to water, stirring the solution fully, and adjusting the pH value; dropwise adding a lipid-soluble vitamin ethanol solution, stirring the solution fully, and carrying out rotary evaporation, thus obtaining the corn peptide glycosylation product nano particles embedding lipid-soluble vitamins. The preparation method has the advantages and beneficial effects that the corn peptide glycosylation product nano particles embedding lipid-soluble vitamins have high lipid-soluble vitamin embedding rates and small particle sizes; the solution has better transparency and pH stability; the corn peptide glycosylation product nano particles can be applied to dairy products and drinks as ingredients; the method is simple in process, low in cost and strong in operability.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural by-products and food industry, and relates to the preparation of corn peptide glycosylated nanoparticles embedding fat-soluble vitamins, which can be used as ingredients in dairy products and beverages. Background technique [0002] Fat-soluble vitamins are essential nutrients for human beings, which are hardly soluble in water but easily soluble in organic solvents. Both vitamin E and vitamin D are fat-soluble vitamins. Due to their poor water solubility, their application in the food industry has been limited. [0003] In recent years, microcapsule technology for embedding hydrophobic active substances has attracted more and more attention. Zein has the characteristics of special solubility and self-assembly to form nano-spherical particles. At the same time, as a natural biological macromolecule, zein has good biocompatibility and biodegradability. Therefore, at prese...

Claims

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Application Information

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IPC IPC(8): A23P1/04A23L2/66A23P10/30
Inventor 杨晓泉任晓鸣王金梅张晋博郭健
Owner SOUTH CHINA UNIV OF TECH
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