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Extraction method of ethyl gallate in peony leaves and new application thereof

A technology of ethyl gallate and extraction method, which is applied in the fields of chemistry and agriculture, and can solve problems such as no agricultural herbicidal activity

Inactive Publication Date: 2013-12-18
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on its agricultural herbicidal activity

Method used

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  • Extraction method of ethyl gallate in peony leaves and new application thereof
  • Extraction method of ethyl gallate in peony leaves and new application thereof
  • Extraction method of ethyl gallate in peony leaves and new application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1 Extraction of Compound Ethyl Gallate

[0018] Take 4000g peony leaf powder, soak and extract 6 times with 80% ethanol solution (8000mL), each time is 3d. Stir every 6 hours during this period. After the extracts were combined, the ethanol was recovered by vacuum filtration and rotary evaporation to obtain 665 g of ethanol extract. Add 640 g of ethanol extraction extract to 20 times the amount of distilled water to suspend, add the same amount of petroleum ether and ethyl acetate to extract 8 times respectively, combine the respective extracts, and then rotatively evaporate to recover the solvent to obtain petroleum ether and ethyl acetate respectively. extract, wherein ethyl acetate extract extract 129g. Get 80g of ethyl acetate extract extractum through silica gel column chromatography, use 1:0, 100:1, 50:1, 25:1, 15:1, 10:1, 5:1, 3:1, 1:1 successively 1 and 0:1 volume ratios of petroleum ether and acetone mixed solvents and 25:1, 10:1, 5:1, 1:1 and 0:...

experiment example 2

[0023] Experimental example 2 Ethyl gallate and its herbicidal activity at each extraction stage

[0024] 1) Experimental materials

[0025] Lettuce, wheat, summer solstice grass, barnyardgrass

[0026] 2) Experimental method

[0027] Agar method: soak the recipient plant seeds in 0.2% sodium hypochlorite solution for 15 minutes, rinse them with tap water for several times and place them under running water to absorb water for about 3~5 hours, then wash them with distilled water for several times, and place them evenly in a square plate with a cover. On absorbent paper, add distilled water until the seeds do not float, and place in a fully intelligent artificial climate box (HP1000GS-B type, Wuhan Ruihua Instrument Equipment Co., Ltd.) to germinate until the radicle (or seed root) reaches 3~5mm transplanted to 0.5% agar gel mixed with extracts of each stage (prepared 100mL, and evenly injected into 3 small beakers), the extracts of each extract should be dissolved in 0.5m...

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Abstract

The invention relates to the field of chemistry and agriculture, and particularly provides an extraction method of ethyl gallate in peony. The method comprises the following steps: extracting the plant dry powder of peony leaves by use of ethanol, petroleum ether and ethyl acetate; performing gradient elution on the ethyl acetate extract through silica gel column chromatography by use of petroleum ether in combination with acetone and acetone in combination with methanol respectively to obtain 8 fractions; and performing gradient elution on the M5 fraction with higher activity by use of petroleum ether in combination with acetone to obtain ethyl gallate. The invention further provides a new application of the ethyl gallate in weeding.

Description

technical field [0001] The invention relates to the fields of chemistry and agriculture, and specifically provides a method for extracting ethyl gallate in tree peony leaves and its application. Background technique [0002] peony( Paeonia suffruticosa Andr.) is a deciduous shrub of Paeoniaceae Paeoniaceae, which is an important ornamental and medicinal plant. Its root bark is a commonly used Chinese medicinal material, and the paeonol contained in it has various pharmacological effects such as antithrombotic, anti-arteriosclerosis, lowering blood pressure, and protecting brain tissue [Lin Bixuan. China Practical Medicine, 2011,6(19):237- 238]. Petals are rich in polyphenols, flavonoids and anthocyanins, which have antioxidant activity and free radical scavenging ability [Feng Zhiwen, Yang Xiaguang, Pan Jian. Journal of Northwest A&F University, 2009, 37(1) 205-209]. Peony seed extract Ⅱ (fat-soluble components) has certain inhibitory effects on Bacillus subtilis, Salmone...

Claims

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Application Information

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IPC IPC(8): C07C69/84C07C67/48A01P13/00
Inventor 罗小勇郭凤
Owner QINGDAO AGRI UNIV
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