Method for rapidly detecting main pathogenic bacteria, namely vibrio splendidus of sea cucumber skin disease

A technology for Vibrio splendidus and skin rot, which is applied in the field of detection of pathogenic bacteria of sea cucumber skin rot syndrome, can solve problems such as restricting popularization and application, and achieve the effects of high sensitivity, strong specificity and low detection cost.
CN103451275AActive Publication Date: 2013-12-18YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
Publication Date
2013-12-18

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Abstract

The invention relates to the technical field of detection of pathogenic bacteria of cultured sea cucumber skin disease syndromes, and particularly relates to a primer for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease. The invention is used for overcoming the defects of the technology for detecting main pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes of the existing sea cucumber, and providing a method for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndrome. The method comprises the following steps: centrifuging, cleaning, freezing and thawing and boiling a pure bacterial culture as a template to carry out LAMP (loop-mediated isothermal amplification); designing an LAMP primer aiming at a vibrio splendidus flgF gene; configuring and optimizing a reaction system; bathing for 45 minutes at the temperature of 65 DEG C, and judging the result by naked eyes or electrophoresis. By adopting the method, the LAMP technology is firstly applied to detection of pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes. The method has the advantages of being short in time consumption, low in cost, simple, convenient and easy to use, suitable for popularization in a basic level, and the like.
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Description

Technical field:

[0001] The invention belongs to the technical field of detection of causative bacteria of sea cucumber rotting skin disease syndrome, and specifically relates to a method for rapidly detecting vibrio splendidus, the main pathogen of sea cucumber rotting skin disease syndrome. Background technique:

[0002] According to incomplete statistics, the direct economic losses caused by aquaculture diseases in our country are as high as tens of billions of yuan every year. Research and development of technologies for diagnosis and analysis of aquaculture diseases, especially early diagnosis and analysis reagents (boxes) for aquaculture diseases, have high potential for accurate and sensitive detection of pathogens of aquaculture animal diseases and early rapid diagnosis of diseases Practical value.

[0003] Sea cucumber rotting skin disease syndrome is a common disease of sea cucumbers. It has the characteristics of wide incidence and involves the sea cucumber culti...

Claims

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