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Poly (9-fluorenemethanol) and detection method of pesticide imidacloprid using poly (9-fluorenemethanol)

A detection method, imidacloprid technology, applied in the field of fluorescent molecular sensing, can solve the problems of imidacloprid molecules not being able to detect fluorescence response, expensive equipment and training costs, bovine serum albumin fluorescence quenching, etc. Inexpensive, low detection limit performance

Active Publication Date: 2013-12-18
NANJING UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many traditional methods for the determination of imidacloprid are not only expensive in terms of equipment and training costs, but also consume a lot of reagents and waste, and the cumbersome multi-step procedures are labor-intensive and time-consuming
The fluorescence method is a fast and convenient detection method, but imidacloprid molecules can hardly detect the fluorescence response, so finding a suitable fluorescent probe to detect imidacloprid is the core of the fluorescence method detection
Zhou Qiuhua and others used bovine serum albumin as a probe, and by studying the interaction between imidacloprid and bovine serum albumin, found that imidacloprid can quench the fluorescence of bovine serum albumin, which can be used for the detection of imidacloprid, but the high price of bovine serum albumin limit its practical application

Method used

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  • Poly (9-fluorenemethanol) and detection method of pesticide imidacloprid using poly (9-fluorenemethanol)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of electrolyte solution: Add 9-fluorenemethanol to boron trifluoride ether to prepare an electrolyte solution with a monomer content of 0.05M, and ultrasonicate for 30 minutes;

[0023] (2) Poly(9-fluorenemethanol) electropolymerization: using a three-electrode system, using platinum as the working electrode and auxiliary electrode, the area ratio of the working electrode and the auxiliary electrode is 1:1.2, and Ag / AgCl as the reference electrode. 1.2V potentiostatic polymerization for 40000s to obtain the crude product of poly(9-fluorenemethanol);

[0024] (3) Pretreatment of poly(9-fluorenemethanol): after the electropolymerization of poly(9-fluorenemethanol), the electrode is taken out from the electrolyte solution, soaked and rinsed repeatedly with anhydrous ether to remove some oligomers or heteroions until the ether is completely clear;

[0025] (4) Dedoping of poly(9-fluorenemethanol): Take out the electrode after (3) cleaning, scrape off the po...

Embodiment 2

[0030] (1) Preparation of electrolyte solution: Add 9-fluorenemethanol to boron trifluoride ether to prepare an electrolyte solution with a monomer content of 0.01M, and sonicate for 10 minutes;

[0031] (2) Poly(9-fluorenemethanol) electropolymerization: a three-electrode system is used, 304 stainless steel is used as the working electrode, platinum is used as the auxiliary electrode, the area ratio of the working electrode and the auxiliary electrode is 1:2, and Ag / AgCl is used as the reference The electrode was polymerized at a constant potential of 1.3V for 20000s to obtain a crude product of poly(9-fluorenemethanol);

[0032] (3) Pretreatment of poly(9-fluorenemethanol): after the electropolymerization of poly(9-fluorenemethanol), the electrode is taken out from the electrolyte solution, soaked and rinsed repeatedly with anhydrous ether to remove some oligomers or heteroions until the ether is completely clear;

[0033] (4) Dedoping of poly(9-fluorenemethanol): Take out ...

Embodiment 3

[0037] (1) Preparation of electrolyte solution: Add 9-fluorenemethanol to boron trifluoride ether to prepare an electrolyte solution with a monomer content of 0.02M, and ultrasonicate for 20 minutes;

[0038] (2) Poly(9-fluorenemethanol) electropolymerization: a three-electrode system was adopted, platinum sheets were used as the working electrode and the auxiliary electrode, the area ratio of the working electrode and the auxiliary electrode was 1:2, and Ag / AgCl was used as the reference electrode. 1.25V potentiostatic polymerization for 30000s to obtain the crude product of poly(9-fluorenemethanol);

[0039] (3) Pretreatment of poly(9-fluorenemethanol): after the electropolymerization of poly(9-fluorenemethanol), the electrode is taken out from the electrolyte solution, soaked and rinsed repeatedly with anhydrous ether to remove some oligomers or heteroions until the ether is completely clear;

[0040] (4) Dedoping of poly(9-fluorenemethanol): Take out the electrode after (...

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Abstract

The invention discloses poly (9-fluorenemethanol) and a detection method of a pesticide imidacloprid using poly(9-fluorenemethanol). The poly(9-fluorenemethanol) is prepared by a method comprising the following steps of by taking 9-fluorenemethanol and boron trifluoride diethyl etherate as raw materials, electrically synthesizing the poly(9-fluorenemethanol) through one step in a three-electrode system through constant-potential polymerization; pretreating the obtained poly(9-fluorenemethanol), wherein the obtained polymer has excellent solubility and excellent fluorescence characteristics in many solvents. A nicotine pesticide imidacloprid has excellent fluorescence quenching effect on the imidacloprid, and can efficiently, sensitively and specifically detect the imidacloprid. The method provided by the invention is used for detecting the residual content of the imidacloprid in the environment, and has the advantages of low synthetic cost, simple process, simple and convenient operation and the like, and the detection limit is 9.1 ngml<-1>. Therefore, the method provided by the invention provides a reliable, convenient-to-implement method for detecting imidacloprid residue in the environment, and is capable of meeting the requirements in research and production; consequently, the method has potential application value and excellent application prospect.

Description

technical field [0001] The invention relates to the field of fluorescent molecular sensing, in particular to a poly(9-fluorenemethanol) and a method for detecting the pesticide imidacloprid. Background technique [0002] 9-fluorenemethanol is a fluorescent small molecule with an aromatic structure, and a more stable poly-9-fluorenemethanol can be obtained through further polymerization. This polymer has a more stable structure and fluorescent properties. Compared with fluorescent small molecules, the fluorescence Polymers have unique advantages: ① Chromophores are doped or chemically bonded in polymers, so they are not easy to fall off; ② Chromophores are evenly distributed, stable in content, and have good luminescence and light-guiding properties. Therefore, in the past research, people combined the excellent luminescent properties of fluorescent small molecules with the good mechanical properties of polymers, and introduced fluorescent small molecules into polymer...

Claims

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Application Information

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IPC IPC(8): C25B3/00C08G61/02G01N21/64
Inventor 雷武司维蒙郝青丽夏明珠王风云
Owner NANJING UNIV OF SCI & TECH