Method for separating and purifying methyl p-hydroxybenzoate and 3-indolylformaldehyde from Trichosanthes kirilowii Maxim stem and leaf

A technology for separation and purification of stems and leaves, applied in the field of separation and purification of methyl p-hydroxybenzoate and 3-indole formaldehyde, which can solve the problems of insufficient utilization of Trichosanthes stems and leaves, few research reports on Trichosanthes stems and leaves, and waste of resources and other problems to achieve the effect of saving reagents, short process cycle and prolonging service life

Inactive Publication Date: 2013-12-25
LIAOCHENG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many reports on the chemical constituents of Trichosanthes chinensis, but there are very few research reports on the stems and leaves of Trichosanthes chinensis
At present, the stems and leaves of Trichosanthes chinensis have not been fully utilized, and most of them are discarded as waste, resulting in a great waste of resources

Method used

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  • Method for separating and purifying methyl p-hydroxybenzoate and 3-indolylformaldehyde from Trichosanthes kirilowii Maxim stem and leaf
  • Method for separating and purifying methyl p-hydroxybenzoate and 3-indolylformaldehyde from Trichosanthes kirilowii Maxim stem and leaf
  • Method for separating and purifying methyl p-hydroxybenzoate and 3-indolylformaldehyde from Trichosanthes kirilowii Maxim stem and leaf

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Embodiment

[0031] Example: The method for separating and purifying methyl p-hydroxybenzoate and 3-indole formaldehyde from stems and leaves of Trichosanthes chinensis, the steps are:

[0032] (1) Preparation of the crude extract from the stems and leaves of Trichosanthes chinensis: after crushing the stems and leaves of Trichosanthes chinensis, heat and reflux extraction with 70% ethanol as solvent for 4 times, combine the extracts, and concentrate under reduced pressure to obtain the crude extract.

[0033] (2) Extraction: Disperse the crude extract of Trichosanthes stems and leaves in water, extract with equal volumes of petroleum ether, ethyl acetate and n-butanol in sequence, and concentrate the layers of petroleum ether, ethyl acetate and n-butanol under reduced pressure respectively to obtain petroleum Ether site, ethyl acetate site and n-butanol site.

[0034] (3) Separation and purification of reverse-phase C18 column: Separation and purification of n-butanol fraction with reve...

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Abstract

The invention relates to a method for separating and purifying methyl p-hydroxybenzoate and 3-indolylformaldehyde from Trichosanthes kirilowii Maxim stem and leaf, which uses Trichosanthes kirilowii Maxim stem and leaf as the raw material and comprises the following step: (1) preparation of Trichosanthes kirilowii Maxim stem and leaf crude extract; (2) extraction; (3) reversed phase C18 column separation and purification; and (4) semipreparative high performance liquid chromatography separation and purification: carrying out separation and purification by semipreparative high performance liquid chromatography by using methanol-water as a mobile phase to obtain the two high-purity components, which are respectively methyl p-hydroxybenzoate and 3-indolylformaldehyde after identification. The technical process is green and environment-friendly, does not have severe damage to the environment, and is low in comprehensive cost.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for separating and purifying methyl p-hydroxybenzoate and 3-indole formaldehyde from stems and leaves of Trichosanthes chinensis. Background technique [0002] Cucurbitaceae Trichosanthes genus Trichosanthes ( Trichosanthes kirilowii Marim.) and Bilateral Trichosanthes ( Trichosanthes rosthorinii Harms) is the original plant of the Trichosanthes medicinal material stipulated in the Chinese Pharmacopoeia, and its fruit, peel, seeds and tubers are used as Trichosanthes, Trichosanthes skin, Trichosanthes seeds and trichosanthes respectively. The stems and leaves of Trichosanthes chinensis are sour, cold in nature, non-toxic, and return to the liver and spleen meridians. They have the effect of clearing away heat and relieving summer heat. Ascites-type cancer cells in the dead body have anti-cancer effects. [0003] There are many reports on the chemical co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/84C07C67/48C07C67/58C07C67/56C07D209/12
Inventor 李爱峰张永清柳仁民孙爱玲
Owner LIAOCHENG UNIV
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