The application discloses a detection method of indole-3-methanal in compound
amino acid injection, and belongs to the field of
analytical chemistry. The detection method adopts
high performance liquid chromatography, and the chromatographic conditions include: a
chromatographic column with octadecylsilane bonded silica as a filler,
isocratic elution with a mobile phase, detection by a
UV detector with a detection
wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a
column temperature of 30-40 DEG C, and a mobile phase of a
mixed solution of
acetonitrile and
phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate,
column temperature,
wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the
impurity by using
high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound
amino acid injection, so that the effectiveness and safety of the related products are improved.