29 results about "Indolecarboxaldehyde" patented technology

The invention belongs to the field of medicines, and provides application of indole-3-formaldehyde in preparation of an enteric nerve protection medicine. A loperamide-induced C57BL / 6J mouse constipation model is adopted, it is found that indole-3-carboxaldehyde can accelerate the small intestine propulsion rate of a model mouse, increase the water content of excrement and the excretion number of excrement particles in unit time and improve the intestinal motility of the model mouse, and HUC / DmRNA expression of mouse colon tissue treated by indole-3-carboxaldehyde is remarkably increased compared with a model group; protein expression of HUC / D and HSP90AA1 is remarkably increased compared with that of a model group, immunohistochemistry and immunofluorescence show that the number of HUC / D dyed neurons and fluorescence intensity of colon tissue treated by indole-3-formaldehyde are remarkably increased compared with that of the model group, and therefore, the indole-3-formaldehyde can promote intestinal motility by protecting HUC / D intestinal neuronal cells.

The invention relates to the field of chemical analysis, and discloses a method for quantitatively detecting 3-indole-acetic acid and 3-indole-formaldehyde in plant root exudates, which comprises the following steps: (1) preparing a series of mixed standard mother liquor of 3-indole-acetic acid and 3-indole-formaldehyde with concentration gradient; (2) drawing a standard curve; (3) preparing a root exudate extracting solution; (4) detecting the root exudate extracting solution in the step (3) by adopting a method which is the same as that in the step (2); and (5) comparing the detection result in the step (4) with the standard sample in the step (2), and determining the content of 3-indole-acetic acid and 3-indole-formaldehyde in the root exudates. The method disclosed by the invention has the advantages of simplicity in operation, high detection speed, short time consumption, small amount of required samples, large linear range of determination, good repeatability and good quantitative effect, can be suitable for samples with large concentration fluctuation range, and is particularly suitable for quantitative detection of root exudates.

The invention discloses a metabolite for treating sarcopenia and application of the metabolite in a health-care product additive, and belongs to the technical field of biomedicine, and the application comprises application of indole-3-formaldehyde in preparation of a medicine for treating sarcopenia. The invention further discloses a health care product additive and a medicine for treating sarcopenia, wherein the medicine comprises indole-3-formaldehyde. According to the present invention, the indole-3-formaldehyde can be adopted as the health product additive or the effective component in the drug, can be used for treating sarcopenia, and can recover the skeletal muscle cell mitochondrial homeostasis so as to improve the muscle cell mitochondrial function index.

The invention discloses an application of indole-3-formaldehyde in preparation of antidepressant drugs, and relates to the technical field of medicines.The antidepressant drugs comprise active ingredients and pharmaceutical excipients, the active ingredients comprise indole-3-formaldehyde or pharmaceutically acceptable salts thereof, and the effective dose of the active ingredients in rats is 70-200 mg / kg; indole-3-formaldehyde is adopted, so that depression-like behaviors of mice can be remarkably improved, the indole-3-formaldehyde can be used as an exogenous oral supplement to enter intestinal tracts and even can penetrate through a blood brain barrier to enter brain tissues, and the anti-depression effect is achieved from the two aspects of improving the intestinal steady state and protecting neuroinflammation. The tryptophan intestinal metabolite is applied to treatment and improvement of depression for the first time, and a theoretical basis and a novel angle are provided for subsequent research and development of antidepressant drugs applying the tryptophan intestinal metabolite.

The present invention relates to a method for synthesizing indole-3-carbaldehyde compounds in a microchannel. Using indole compounds as substrate raw materials, phosphorus oxychloride as a catalyst, and DMF as an aldehyde group source and solvent, a formylation reaction is carried out through a microchannel reactor to obtain the target product indole-3-carbaldehyde compounds. Compared with the prior art, the process route of the present invention is simple, efficient, and low-cost. The reaction is carried out at room temperature, shortening the reaction time, increasing the reaction yield, greatly reducing the reaction energy consumption, and facilitating continuous production on a kilogram scale.

The present application mainly relates to a kind of a-karoline derivatives and its synthesis method, under the action of iodine reagent and organic solvent, with indole-3-formaldehyde compound, inorganic ammonium salt, alkyne and other simple and easily obtained raw materials one-step synthesis a-karoline derivatives technical scheme;It overcomes the problems in the prior art a-karoline derivatives synthesis method, such as the need to use expensive metal catalyst, or complex synthesis process, or the need to use high-activity complex reaction substrate etc.It is worth mentioning that the present application can be applied to gram-scale one-step synthesis of a-karoline compound containing antitumor activity.The present application greatly maintains atomic economy;With simple reaction system, high selectivity, low raw material cost and easy to obtain, less reaction equipment, simple experimental operation, high yield, high product utilization value, potential application value in the field of medicine.

The application discloses a fermented Lactobacillus mui J0273, which is preserved in the Guangdong Microbial Culture Collection Center on March 28, 2024, and has a preservation number of GDMCC No: 64463; the fermented Lactobacillus mui J0273 provided by the application has antioxidant activity, can produce indole-3-methanol, and has good DPPH free radical clearance rate, hydroxyl radical clearance rate, high reducing power, Fe 2+ Chelating capacity, can enhance the antioxidant capacity of the host, regulate the host immune, protect the intestinal barrier, and relieve inflammation.

The present disclosure is directed to a method of treating a CTLA-4 checkpoint associated immunodeficiency by administering a therapeutically effective amount of a pharmaceutical composition comprising 1H-indole-3-carboxaldehyde (3-IAld) or 1-methylindole-3-carboxylic acid.

The invention discloses a method for constructing a near-infrared first-zone boron-dipyrromethene dye conjugate through a Knoevenagel condensation reaction and a preparation method of the near-infrared first-zone boron-dipyrromethene dye conjugate. According to the method, 5, 5-difluoro-1, 3, 7, 9-tetramethyl-10-(trifluoromethyl)-5H-4 lambda, 5 lambda-dipyrrolo [1, 2-c: 2 ', 1'-f] [1, 3, 2] diazaborocyclohexane meso-CF3-BODIPY is taken as a raw material, 5-hydroxyindole-3-formaldehyde or 3-indoleformaldehyde or 6-hydroxyindole-3-formaldehyde is taken as a reactant, acetic acid and piperidine are taken as catalysts, and 1, 4 dioxane is taken as a solvent, so that the meso-CF3-BODIPY conjugate is prepared. The method has the advantages of mild reaction conditions, easily available raw materials and excellent yield, and provides a new method for synthesizing the BODIPY conjugate.

The invention belongs to the technical field of photoelectrochemical aptamer sensors, and provides a polarity turnover type photoelectrode constructed by polyindole-5-formaldehyde, and a preparation method and application thereof. A substrate electrode of the polarity reversal type photoelectrode is an ITO (Indium Tin Oxide) electrode, and the surface of the ITO electrode is sequentially modified with a WO3 / Pt composite material, indole-5-formaldehyde, an ochratoxin A aptamer and bovine serum albumin to obtain the polarity reversal type photoelectrode. The PEC polarity reversal type aptamer sensor is prepared based on WO3 / Pt and by introducing P5FIn to construct a Z-type heterojunction, the PEC aptamer sensor can eliminate false negative or false positive signals brought by interfering substances, the accuracy of the PEC sensor is improved, the PEC aptamer sensor is used for detecting ochratoxin A, the detection limit is as low as 0.0229 pg.mL <-1 > (S / N = 3), and the application prospect is wide. A novel method is provided for quantitative detection of the ochratoxin A.

The invention belongs to the technical field of biology, and particularly relates to escherichia coli recombinant engineering bacteria and application thereof. The escherichia coli recombinant engineering bacterium is escherichia coli F10 and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2024964; the Escherichia coli F10 can generate indole-3-formaldehyde and indole trimer metabolites in the fermentation process, and can be used for preparing the indole-3-formaldehyde and the indole trimer. The invention also provides a preparation method of the indole compound, which specifically comprises the following steps: carrying out fermentation culture on Escherichia coli F10 to obtain fermentation liquor, centrifuging the fermentation liquor, collecting supernatant, adding ethyl acetate into the supernatant for extraction, collecting supernatant after the extraction is finished, and concentrating the supernatant to obtain an extract; carrying out silica gel column chromatography separation on the extract, and collecting the elution fraction of silica gel column chromatography; the elution fraction is subjected to gel column chromatography and high performance liquid chromatography separation and purification, and the indole compounds are obtained. Compared with an existing preparation method through a chemical process, the preparation method of the indole compound is green and environment-friendly, simple in reaction condition, few in by-product and high in product purity.

The invention belongs to the technical field of medicines, and particularly relates to an application of indole-3-formaldehyde in prevention or treatment of rheumatoid arthritis, and the indole-3-formaldehyde has no side effects such as kidney damage, hepatotoxicity, gastrointestinal reaction and the like in treatment of inflammatory reactions of mice with collagen-induced rheumatoid arthritis. In addition, the indole-3-formaldehyde provided by the invention also has the effect of protecting the intestinal mucosal barrier. The indole-3-formaldehyde is an endogenous metabolite of a human body, and is safe to organisms; and secondly, the indole-3-formaldehyde can be combined with probiotics such as bifidobacterium, lactobacillus casei and the like and prebiotics such as fructo-oligosaccharide, galactooligosaccharide, inulin and the like for use, so that the conflict emotion of a patient to the medicine is reduced, and the indole-3-formaldehyde has a good application prospect in clinic.

The invention mainly relates to the technical field of drug analysis and detection, in particular to an impurity detection method of a compound amino acid injection. High performance liquid chromatography is adopted for detection, and chromatographic conditions are as follows: a chromatographic column adopts octadecylsilane chemically bonded silica as a filler; the wavelength of a UV detector is 210 to 225 nm; mobile phases: a mobile phase A: a sodium dihydrogen phosphate solution with a pH value of 4.0-6.5 and methanol; a mobile phase B: acetonitrile and water; the elution mode is gradient elution; the impurities comprise a tryptophan impurity B, a tryptophan impurity C, a tryptophan impurity D, a tryptophan impurity E, a tryptophan impurity H, a tryptophan impurity G and indole-3-formaldehyde. The detection method disclosed by the invention is good in specificity and high in accuracy; different detection methods do not need to be adopted for separate detection, multiple impurities in the preparation can be detected at the same time, and the detection frequency is reduced; the detection time is shortened and the detection cost is saved; a reliable method is provided for the preparation process of the compound amino acid injection and the quality control of the product, and the medication safety of a patient is improved.

The invention discloses application of indole-3-formaldehyde in preparation of a medicine for treating pulmonary arterial hypertension, and belongs to the technical field of medicines. The indole-3-formaldehyde disclosed by the invention can be used for effectively relieving related pathological changes of rats with pulmonary arterial hypertension. Therefore, the indole-3-formaldehyde has a huge application prospect in preventing and / or relieving pulmonary arterial hypertension related diseases. The invention provides a new drug choice for the treatment of pulmonary arterial hypertension, and has significant clinical transformation potential.

The application discloses a detection method of indole-3-methanal in compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts high performance liquid chromatography, and the chromatographic conditions include: a chromatographic column with octadecylsilane bonded silica as a filler, isocratic elution with a mobile phase, detection by a UV detector with a detection wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a column temperature of 30-40 DEG C, and a mobile phase of a mixed solution of acetonitrile and phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate, column temperature, wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the impurity by using high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound amino acid injection, so that the effectiveness and safety of the related products are improved.

The application provides a pretreatment method and a detection method of indole substances in aquatic products, and relates to the technical field of analysis and detection. The application extracts the aquatic products to be measured by using formic acid-acetonitrile aqueous solution as an extractant, can realize the simultaneous and efficient extraction of six indole substances, i.e. indole, 3-indole acetic acid, 3-methyl indole, indole-3-methyl aldehyde, indole-4-methyl aldehyde and indole-5-methyl aldehyde; the HLB solid phase extraction purification can significantly reduce the amount of impurities in the sample liquid to be measured, and improve the accuracy of the detection result of the subsequent high performance liquid chromatography tandem mass spectrometry. The application adopts the high performance liquid chromatography tandem mass spectrometry detection and the APCI ion source, and the detection result is high in accuracy and sensitivity. Moreover, the detection method provided by the application realizes the high sensitivity detection of the six indole substances, is comprehensive in detection indexes, and can provide the basis for the rapid evaluation of the quality of the aquatic products under the cold storage condition according to the change trend of the total content of the six indole substances.

The invention mainly relates to a synthesis method of a 2-aryl-9H-pyrimido [4, 5-b] indole derivative, which comprises the following steps: under the combined action of a copper catalyst, a ligand, an oxidant and an additive which are cheap and easy to obtain, carrying out condensation cyclization reaction on an indole-3-formaldehyde compound and an amidine compound to construct two new C-N bonds, and synthesizing the 2-aryl-9H-pyrimido [4, 5-b] indole derivative in one pot. 5-b] indole derivatives. The method provided by the invention overcomes the defects that the existing synthetic method of the 2-aryl-9H-pyrimido [4, 5-b] indole compound has complex synthetic steps and needs to adopt a multi-step synthetic process; the method has the advantages of simple reaction system, less reaction equipment, simplicity and convenience in experimental operation, wide material source, easiness in expansion of users and application, higher product utilization value and the like.

The invention discloses a method for detecting indole-3-formaldehyde in a compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts a high performance liquid chromatography, and the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a chromatographic column of a filler, a mobile phase is used for isocratic elution, an ultraviolet detector is used for detection, the detection wavelength is 301-311nm, the flow rate of the mobile phase is 0.6-1.0 mL / min, the sample size is 50-150mu L, the column temperature is 30-40 DEG C, and the mobile phase is a mixed solution of acetonitrile and a phosphate buffer solution. According to the present invention, by using the high performance liquid chromatography, combining the characteristics of low impurity response and small polarity, the chromatographic conditions such as the mobile phase ratio, the pH value, the flow rate, the column temperature, the wavelength, the sample injection amount and the like are pertinently selected, and the systematic methodology verification results show that the advantages of strong specificity, high sensitivity, good precision, strong durability and the like are provided; the indole-3-formaldehyde in the compound amino acid injection can be qualitatively or quantitatively detected, so that the effectiveness and safety of related products are improved.

The invention discloses application of indole-3-formaldehyde in preparation of a product for treating type-2 diabetes mellitus complicated with metabolic dysfunction related steatohepatitis, and belongs to the technical field of biological medicine. According to the application disclosed by the invention, a T2DM-MASH mouse model is successfully established, not only is the effect of the type 2 diabetes mellitus in acceleration of MASLD fibrosis progress proved, but also a dual treatment mechanism of indole-3-formaldehyde (3-IAld) on the type 2 diabetes mellitus complicated with metabolic dysfunction related steatohepatitis is clarified for the first time; meanwhile, SIRT1 is activated to inhibit a TGF-beta / SMAD signal channel, so that the effect of treating type-2 diabetes mellitus complicated with metabolic dysfunction related steatohepatitis is achieved. These findings provide new therapeutic targets and strategies for the treatment of type-2 diabetes mellitus complicated with metabolic dysfunction related steatohepatitis.

The invention discloses an indole-fused 3-thiopyrazolo[1,5-a]pyrimidine compound, a synthesis method, and an application thereof. The synthesis method of the indole-fused 3-thiopyrazolo[1,5-a]pyrimidine compound comprises the following steps: mixing a 3-indole formaldehyde compound, a 3-aminopyrazole compound, a disulfide, and an iodine-containing additive in a solvent for reaction to obtain an indole-fused 3-thiopyrazolo[1,5-a]pyrimidine compound. The present invention uses a 3-aminopyrazole compound, an aromatic aldehyde derivative, and a thioether as starting materials to prepare the indole-fused 3-thiopyrazolo[1,5-a]pyrimidine compound in one pot. The reaction conditions are mild, the raw materials are cheap and readily available, and the operation is simple, which can significantly reduce production costs and improve production safety. The indole-fused 3-thiopyrazolo[1,5-a]pyrimidine compound has good anti-tumor properties.

The invention belongs to the field of extraction of active ingredients, and particularly relates to a preparation method for separating an anti-vascular endothelial injury active ingredient in spina gleditsiae based on high-speed counter-current chromatography, the obtained active ingredient and application. The method comprises the following steps: (1) taking spina gleditsiae, and adding ethanol for cold soaking to obtain a crude extract; (2) sequentially extracting the crude extract with a solvent to obtain a chloroform extract; and (3) carrying out high-speed counter-current chromatography on the chloroform extract to prepare compounds 1-4 at one time. According to the preparation method provided by the invention, the indole-3-formaldehyde, the (-)-syringol, the 4, 5-dihydroxybenzenol and the omega-hydroxypropylguaiacol can be obtained through large-scale, efficient and rapid purification; and the mobile phase and the stationary phase in the preparation process can be repeatedly utilized, so that the preparation cost is saved.

The invention discloses a synthetic method for direct diarylation of indole C2 and C4 positions, and relates to the technical field of chemical organic synthesis. The invention discloses a synthesis method of a 10-phenylbenzo [4, 5] isothiazolo [2, 3-a] indole-5, 5-dioxide compound, which comprises the following steps: in the presence of a transition metal catalyst, an organic catalyst, an oxidizing agent and acid, taking indole-3-formaldehyde protected by aryl sulfonyl and aromatic hydrocarbon as raw materials, and synthesizing the 10-phenylbenzo [4, 5] isothiazolo [2, 3-a] indole-5, 5-dioxide compound through one-step reaction. The reaction operation is simple, the synthesis steps are short, and synthesis only needs one-time operation. The invention solves the problems of more reaction steps, high cost and poor selectivity of the existing synthesis method of the arylated indole derivative.

The invention belongs to the technical field of vegetation restoration, and relates to a novel plant growth regulator, in particular to a novel plant growth regulator containing an indole structure as well as a preparation method and application of the novel plant growth regulator. The plant growth regulator provided by the invention is an indole compound synthesized by taking o-phenylenediamine and indole-3-formaldehyde as raw materials, and crop growth can be promoted or inhibited under different concentration conditions by applying regulator solutions with different concentrations. And IYAA can generate complexation with copper by virtue of the structural characteristics of IYAA, so that the growth of plants under copper stress is promoted. The regulator provided by the invention is simple in preparation method, obvious in effect and wide in application prospect.

The invention relates to an indole oxazole derivative as well as a preparation method and application thereof. Indole-3-formaldehyde is used as a raw material, benzenesulfonyl chloride is used for activating carboxyl, then Van Lensen reaction is carried out to synthesize an oxazole ring, and nucleophilic substitution reaction is carried out to synthesize a series of indole oxazole derivatives. The synthesis method is simple, and the product is high in yield and purity. The compound has good inhibition effects on pyricularia oryzae, botrytis cinerea, colletotrichum capsici, rhizoctonia solani, fusarium graminearum and sclerotinia sclerotiorum, and has a good agricultural application prospect.

This invention discloses a non-heavy atom near-infrared two-photon photosensitive dye, TMBDP, its preparation method, and its applications. This TMBDP photosensitive dye contains no heavy atoms, possesses a fluoroboron dipyrrole dimer structure and two-photon absorption properties, and can simultaneously generate singlet oxygen and superoxide anion radicals through a spin vibrational coupling mechanism. The non-heavy atom two-photon photosensitive dye TMBDP is prepared from triphenylamine fluoroboron dipyrrole dimer and 1-(2-morpholinoethyl)-1H-indole-3-carboxaldehyde via a multi-step tandem reaction. Under near-infrared laser excitation, the non-heavy atom two-photon photosensitive dye TMBDP effectively releases reactive oxygen species through two-photon absorption, promoting tumor cell apoptosis through oxidative damage. As a high-performance near-infrared non-heavy atom two-photon photosensitive dye, it has wide applications in two-photon photodynamic therapy, two-photon fluorescence imaging, and two-photon fluorescence labeling.

The present disclosure is directed to a method of treating a CTLA-4 checkpoint related immunodeficiency by administering a therapeutically effective amount of a pharmaceutical composition comprising 1H-indole-3-carboxaldehyde (3-IAld) or 1-methylindole-3-carboxylic acid.

The invention discloses an anti-wrinkle non-ironing fabric and a preparation method thereof, and relates to the technical field of fabrics. When the anti-wrinkle non-ironing fabric is prepared, the viscose fabric reacts with the ultraviolet absorbent and the epoxy chloropropane and then reacts with the flame retardant to prepare the pre-modified fabric; the preparation method comprises the following steps: carrying out a reaction on 3-indole formaldehyde and 3-amino-1, 5-pentanediol to prepare indolyl diol; the preparation method comprises the following steps: reacting indolyl glycol, polytetrahydrofuran glycol, 2, 2-dimethylolpropionic acid and isophorone diisocyanate, and then reacting with triethylamine to prepare a polyurethane emulsion; preparing a waterborne polyurethane finishing liquid from the polyurethane emulsion and sodium chloride; the pre-modified fabric and the waterborne polyurethane finishing liquid are subjected to a reaction, and the anti-wrinkle non-ironing fabric is prepared. The anti-wrinkle non-ironing fabric prepared by the invention has excellent anti-ultraviolet, flame-retardant, anti-wrinkle and tensile properties.

The invention provides a method for synthesizing a lignin-based carbazole derivative by a one-pot two-step method, and belongs to the technical field of compound synthesis. The method comprises the following steps: step 1, taking a lignin beta-O-4 model compound and an N-methyl-3-indole formaldehyde compound as reaction raw materials, and reacting for a certain time in a solvent under the action of alkali; and 2, adding acetic acid, a Fe-based catalyst, 2, 2, 6, 6-tetramethylpiperidine oxide, a 4-chlorobutanone compound and an additive into the system, and reacting for a certain time to finally obtain the carbazole compound. The synthetic method for preparing the carbazole derivative has the advantages of being economical, low in cost, simple to operate, mild in reaction condition, high in product selectivity and the like, and a novel green way is provided for preparation of carbazole compounds.

This invention belongs to the field of vegetation restoration technology, and relates to a novel plant growth regulator, specifically a novel plant growth regulator containing an indole structure, its preparation method, and its applications. The plant growth regulator provided by this invention is an indole compound synthesized from o-phenylenediamine and indole-3-carboxaldehyde. By applying regulator solutions of different concentrations, it can promote or inhibit crop growth under different concentration conditions. Furthermore, due to its structural characteristics, IYAA can form a complex with copper, promoting plant growth under copper stress. The regulator preparation method provided by this invention is simple, has significant effects, and has broad application prospects.