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Method for extracting salidroside from rhodiola

A technology of salidroside and rhodiola rosea, applied in the field of extracting salidroside, which can solve the problems of inconvenient process operation, long processing time, high cost, etc., and achieve good application prospects, high yield and product purity, and easy operation Effect

Active Publication Date: 2013-12-25
内蒙古泓兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Column chromatography can obtain relatively high-purity salidroside, but the loss of active ingredients is serious and the yield is too low, so it is only suitable for the preparation of trace standards; during the use of ultrafiltration purification ultrafiltration membranes, membrane fouling is easy to cause The flux of the membrane drops sharply, and a large amount of alkali and acid are used in the membrane cleaning process, and the separation selectivity is poor, and the product quality is unstable; while the ethanol precipitation method removes impurities, the obtained dry extract has a high viscosity and is easy to absorb moisture , brings inconvenience to the post-process operation, this method consumes a lot of alcohol, has high cost, and takes a long time to process; the macroporous resin adsorption method is more commonly used, but only relying on a separation method of macroporous adsorbent resin, the obtained Rhodiola Glycoside product purity is still low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 5kg of Rhodiola rosea, crush it, add 30L of water, soak at 40°C for 1h, ultrasonically extract at 65°C for 60min, and then extract twice, adding 30L of extraction solvent water each time. The extracts are combined and filtered, and the obtained filtrate A is preliminarily purified through a macroporous adsorption resin. The macroporous adsorption resin uses AB-8 as the resin column. After washing with 2 times the column volume of water to remove impurities, use 3 times the column volume of 70% ethanol to elute salidroside. The obtained eluate B was concentrated to dryness under reduced pressure to obtain about 109 g of dry product C. The dried substance C was dissolved with 1090ml of absolute ethanol, and the resulting solution entered the simulated moving bed chromatography. The adsorbent filled in the simulated moving bed is silica gel, and the eluent is ethanol-ethyl acetate solution with a volume ratio equal to 1:9; the flow rate in the adsorption zone is 2BV...

Embodiment 2

[0023] Weigh 8kg of Rhodiola rosea, crush it, add 80L of water, soak at 40°C for 1h, ultrasonically extract at 70°C for 60min, and then extract once more, adding 80L of extraction solvent water. The extracts are combined and filtered, and the obtained filtrate A is preliminarily purified through a macroporous adsorption resin. The macroporous adsorption resin uses D101 as the resin column. After washing with 2 times the column volume of water to remove impurities, use 3 times the column volume of 70% ethanol to elute salidroside. The obtained eluate B was concentrated to dryness under reduced pressure to obtain about 162 g of dry product C. The dried substance C was dissolved with 1620ml of absolute ethanol, and the resulting solution entered the simulated moving bed chromatography. The adsorbent filled in the simulated moving bed is silica gel, and the eluent is ethanol-ethyl acetate solution with a volume ratio equal to 1:9; the flow rate in the adsorption zone is 2BV / h; th...

Embodiment 3

[0025] Weigh 5kg of Rhodiola rosea xiaocong, crush it, add 30L of water, soak at 40°C for 1h, ultrasonically extract at 70°C for 65min, and then extract twice, adding 30L of extraction solvent water each time. The extracts are combined and filtered, and the obtained filtrate A is preliminarily purified through a macroporous adsorption resin. The macroporous adsorption resin uses D101 as the resin column. After washing with 2 times the column volume of water to remove impurities, use 3 times the column volume of 70% ethanol to elute salidroside. The obtained eluate B was concentrated to dryness under reduced pressure to obtain 76.2 g of dry product C. Then 762ml of absolute ethanol was used to dissolve the dried substance C, and the resulting solution entered the simulated moving bed chromatography. The adsorbent filled in the simulated moving bed is silica gel, and the eluent is ethanol-ethyl acetate solution with a volume ratio equal to 1:9; the flow rate in the adsorption z...

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Abstract

The invention discloses a method for extracting salidroside from rhodiola. The method for extracting the salidroside from the rhodiola comprises the following steps of (1) smashing a rhodiola medicinal material, using water as solvent, carrying out ultrasonic-assisted extraction for 1-4 times, combining extracting solutions, and filtering the combined solutions to obtain filtrate A; (2) enabling the filtrate A to pass through a macroporous resin column, collecting eluant B of the salidroside, and carrying out vacuum concentration on the eluant B to obtain a dried object C; (3) dissolving the dried object C with absolute ethyl alcohol, carrying out separation on the dried object C through simulated moving bed chromatography, and obtaining a constituent D rich in the salidroside; (4) carrying out concentration, crystallization, centrifugation and drying to obtain a salidroside product. The method for extracting the salidroside from the rhodiola is easy and convenient to operate, broad in application prospect in industries, and high in yield and product purity; according to the technical scheme, the yield of the obtained salidroside reaches more than 70%, and the content of the salidroside contained in the obtained product is more than 98%.

Description

technical field [0001] The invention relates to a method for extracting salidroside from Rhodiola rosea. Background technique [0002] Rhodiola (Rhodiola) is a perennial herb or subshrub belonging to Rhodiola in the Sedum family. It is one of the rare medicinal plants and is known as "Plateau Ginseng". There are about 90 species of this genus in the world, and more than 70 species are distributed in my country, mainly in North China, Northeast China, Southwest China, Northwest China and Central China. Its chemical components mainly include flavonoids, tyrosol, salidroside, sitosterol, organic acids, volatile oils, polysaccharides, fats, proteins, etc. Among them, salidroside and its aglycone tyrosol are the main active ingredients of Rhodiola rosea, and they are also the most important indicators for evaluating Rhodiola rosea and its extracts. Modern pharmacological studies have confirmed that Rhodiola extract has anti-aging, anti-tumor, anti-virus, anti-bacterial, anti-hy...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
Inventor 白易赵永强马亚琼廖小雪贾洪涛
Owner 内蒙古泓兴生物科技有限公司
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