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Short peptide, cooper loading nanometer biological material and application of cooper loading nanometer biological material in cerebral infarction treatment drug preparation

A biomaterial and copper-loaded technology, which is applied in drug combinations, peptides, and decapeptides, can solve problems such as ineffectiveness, aggravation of the disease, and ischemia-reperfusion injury, and achieve neurological function restoration, significant therapeutic effects, and cerebral infarction area. Reduced effect

Inactive Publication Date: 2015-07-08
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, thrombolytic therapy has a strict time window (<3h), and less than 5% of patients with cerebral infarction can receive thrombolytic therapy clinically
If thrombolytic therapy exceeds the time window, it will lead to ischemia-reperfusion injury, which will further aggravate the condition
At present, various neuroprotective drugs that have been proven effective by basic experiments have little effect once they are applied clinically

Method used

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  • Short peptide, cooper loading nanometer biological material and application of cooper loading nanometer biological material in cerebral infarction treatment drug preparation
  • Short peptide, cooper loading nanometer biological material and application of cooper loading nanometer biological material in cerebral infarction treatment drug preparation
  • Short peptide, cooper loading nanometer biological material and application of cooper loading nanometer biological material in cerebral infarction treatment drug preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the synthesis of self-assembled short peptide RP

[0035] 1. Materials and equipment

[0036] 1.1 Reagents

[0037] Fluorenylmethoxycarbonyl (Fmoc)-amino acid is a product of SIAM in the United States; PyBOP, Boc-His-Merrifield resin, hexahydropyridine, and lutidine are products of Merck; dimethylformamide (DMF) is a product of Samsung, Japan Products (soaked in ninhydrin and dehydrated with 3? molecular sieves before use, and determined to be free of free amino groups); dichloromethane (DCM), a product of China National Pharmaceutical (Group) Shanghai Chemical Reagent Co. treatment); trifluoroacetic acid (TFA) is a product of GEEL BELGIUM; methanol is a product of Shanghai Zhenxing Chemical Plant No. 1; HPLC methanol is a product of Merck; tetrahydrofuran is a product of Shanghai Chemical Reagent Station Central Chemical Plant.

[0038] 1.2 Instruments

[0039] The 431A peptide synthesizer is a product of Applied biosystems, the high-performance liquid...

Embodiment 2

[0071] Embodiment 2: the synthesis of RAD16-II short peptide

[0072] 1. Materials and equipment

[0073] 1.1 Reagents

[0074] Fluorenylmethoxycarbonyl (Fmoc)-amino acid is a product of SIAM in the United States; PyBOP, Boc-His-Merrifield resin, hexahydropyridine, and lutidine are products of Merck; dimethylformamide (DMF) is a product of Samsung, Japan The product (soaked in ninhydrin and dehydrated with 3? molecular sieve before use, and determined to be free of free amino groups); dichloromethane (DCM) is a product of China Pharmaceutical (Group) Shanghai Chemical Reagent Co. soaking treatment); trifluoroacetic acid (TFA) is a product of GEEL BELGIUM; methanol is a product of Shanghai Zhenxing Chemical Plant No. 1; HPLC methanol is a product of Merck; tetrahydrofuran is a product of Shanghai Chemical Reagent Station Central Chemical Factory.

[0075] 1.2 Instruments

[0076]The 431A peptide synthesizer is a product of Applied biosystems, the high-performance liquid chro...

Embodiment 3

[0108] Embodiment 3: Preparation of copper-loaded nano-biological material

[0109] 1. Reagents and equipment

[0110] 1.1 Reagents: RP self-assembled short peptide prepared in Example 1, RAD16-II self-assembled short peptide prepared in Example 2; CuSO 4 Analytical grade; deionized water (18 MΩ).

[0111] 1.2 Instruments: ultra-clean bench; ultrasonic instrument; 4 ℃ refrigerator.

[0112] 2. Preparation method

[0113] (1) Sterilization of deionized water

[0114] Take 500 ml of deionized water (18 MΩ) in the pure water system (Millipore Milli-Q system), and use the standard process of autoclaving to sterilize, that is, the deionized water is sterilized at a vapor pressure of 103.4kPa and a temperature of 121.3°C Treat for 30 minutes, then seal and store sterilized deionized water in a refrigerator at 4°C for later use;

[0115] (2) Prepare CuSO 4 sterile stock solution

[0116] At room temperature and pressure, the CuSO 4 Prepare CuSO at a concentration of 40 mM wit...

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Abstract

The invention relates to a self-assembling short peptide, which has an amino acid sequence represented by SEQ ID NO:1 in the sequence table. The invention relates to a copper loading nanometer biological material, which is a gelatinous substance formed by a mixing solution, wherein the mixing solution is prepared from a RP self-assembling short peptide, a RAD16-II self-assembling short peptide, CuSO4 and sterile deionized water, a RP short peptide concentration is 0.1-4 mM, a RAD16-II self-assembling short peptide concentration is 2-8 mM, a CuSO4 concentration is 10-100 muM, an amino acid sequence of the RP short peptide is represented by SEQ ID NO:1 in the sequence table, and an amino acid sequence of the RAD16-II self-assembling short peptide is represented by SEQ ID NO:2 in the sequence table. Experiment results show that the copper loading nanometer biological material can be applied in preparation of cerebral infarction treatment drugs.

Description

technical field [0001] The invention belongs to the field of biomaterials, in particular to self-assembled short peptides, copper-loaded nano-biological materials containing self-assembled short peptides and their application in preparing medicines for treating cerebral infarction. Background technique [0002] Self-assembled short peptide is a new type of nano-biological material. Since its appearance in the 1990s, it has received extensive attention and has been developed rapidly. It has been used in three-dimensional cell culture and preparation of hydrophobic drug carriers, hemostatic drugs, and burn treatment Drugs, antibacterial drugs, leukemia treatment or preventive drugs and other applications. However, the preparation of more self-assembled short peptides and the expansion of the application range of self-assembled short peptides are still issues of concern to scientific and technological workers. [0003] Stroke is the third most deadly disease in the world and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K38/16A61P9/10A61K33/34
Inventor 唐成康康裕建李进
Owner WEST CHINA HOSPITAL SICHUAN UNIV