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Novel magnetic agarose microspheres

A technology of agarose microspheres and magnetism, applied in the separation and purification of biomolecules, and in the field of biomaterials, can solve the problems of limited practical application, decreased performance, and decreased magnetic properties, and achieve the effect of improving poor magnetic response performance

Active Publication Date: 2014-01-01
BEAVERNANO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Since the agarose shell is porous, nano-Fe 3 o 4 And nano-Fe as a magnetic substance embedded in agarose to form magnetic agarose microspheres, because nano-Fe 3 o 4 And nano-Fe has small particle size and large surface area, and it is easy to react with acid under external acidic conditions to cause iron leakage, resulting in a decrease in magnetic properties. Therefore, its acid resistance and oxidation resistance are poor, which limits its practical application;
[0005] (2) Micron-sized stainless steel particles and NdFeB alloy particles are embedded in agarose as magnetic substances to form magnetic agarose microspheres. Although the acid and alkali resistance and oxidation resistance have been improved, due to the large particle size and high density , very easy to settle, mainly used in the field of expanded beds, narrow application range, and easy to magnetize in the magnetic field with remanence, resulting in easy magnetic attraction and agglomeration between magnetic agarose microspheres, and not easy to disperse;

Method used

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Experimental program
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Effect test

Embodiment 1

[0025] with Fe 3 o 4 / SiO 2 Preparation of magnetic agarose microspheres as magnetic cores:

[0026] Use a 500mL graduated cylinder to measure 400mL of cyclohexane into a 1000mL three-necked flask, then add 4mL of Span 85, place the three-necked flask in a constant temperature water bath at 60°C, and stir it electrically for 30 minutes.

[0027] Use an analytical balance to weigh 6.0g of agarose powder and add it to a 500mL conical flask, then add 100mL of Fe 3 o 4 / SiO 2 Content is the aqueous solution of 5mg / mL, and above-mentioned solution is heated to dissolve; Adjust the stirring velocity in above-mentioned cyclohexane oil phase to be 1600rpm, will prepare the obtained Fe-containing 3 o 4 / SiO 2 Slowly add the agarose solution in the cyclohexane oil phase, and keep stirring at 1600rpm for 60 minutes after the addition, and finally stop the water bath heating, draw out the hot water in the water bath, add ice water to cool for 10 minutes to solidify the microspheres...

Embodiment 2

[0029] with Fe 3 o 4 Preparation of magnetic agarose microspheres with / PGMA (polyglycidyl methacrylate) as the magnetic core:

[0030] The mode of reference embodiment 1 uses Fe 3 o 4 / PGMA instead of Fe 3 o 4 / SiO 2 As a magnetic core, embedded Fe with a particle size of 20 μm to 165 μm can be obtained 3 o 4 / PGMA magnetic agarose microspheres.

Embodiment 3

[0032] with Fe 3 o 4 / SiO 2 Preparation of magnetic agarose microspheres as magnetic cores:

[0033] Use a 500mL graduated cylinder to measure 400mL of cyclohexane into a 1000mL three-necked flask, then add 8mL of Span 85, place the three-necked flask in a constant temperature water bath at 70°C, and stir for 30 minutes with electric machinery.

[0034] Use an analytical balance to weigh 6.0g of agarose powder and add it to a 500mL conical flask, then add 100mL of Fe 3 o 4 / SiO 2 An aqueous solution with a content of 5mg / mL was heated to dissolve; finally, the stirring speed in the cyclohexane oil phase was adjusted to 2000rpm, and the prepared agarose solution was slowly added to the oil phase, and after the addition, the stirring speed was maintained at 2000rpm for 60min, and finally Stop heating in the water bath, extract the hot water in the water bath, add ice water to cool for 10 minutes to solidify the microspheres, and wash and purify the solidified microspheres to ...

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Abstract

The invention discloses novel magnetic agarose microspheres. Compared with other magnetic agarose microspheres, the novel magnetic agarose microspheres are characterized in that firstly, superparamagnetic Fe3O4 particles with high specific magnetism serve as magnetic materials, and the fact that the novel magnetic agarose microspheres have high magnetic response performance and superparamagnetism is guaranteed; secondly, magnetic cores of a core / shell structure formed by shell layers with chemical inertness, hydrophilia and biocompatibility wrap the superparamagnetic Fe3O4 particles, and then agarose microspheres are filled with the magnetic cores to obtain the magnetic agarose microspheres. The novel magnetic agarose microspheres resolve the problem that response performance of the magnetic agarose microspheres is poor or residual magnetism is easily produced in a magnetic field, the shell layers with the chemical inertness, the hydrophilia and the biocompatibility wrap the superparamagnetic Fe3O4 particles so as to enable acid and alkali resistance, oxidation resistance and resistance to other kinds of solvent of the Fe3O4 particles to be significantly improved, the fact that the magnetic agarose microspheres have good biocompatibility is guaranteed, and practical application performance is extremely improved.

Description

technical field [0001] The invention relates to a novel magnetic agarose microsphere, which belongs to the field of biomaterials, in particular to the field of separation and purification of biomolecules. Background technique [0002] In recent years, magnetic polysaccharide microspheres have been widely used in the fields of biology, chemistry, pharmacy, and medicine. Their main applications include separation and purification of biomolecules such as proteins, antibodies, and enzymes, targeted drugs, immunoassays, cell separation, and chemical analysis. Agarose gel is a natural polysaccharide chromatography medium. It has many characteristics of an ideal medium, such as: high hydrophilicity, porosity, more activatable hydroxyl groups, and no non-specific adsorption with biological macromolecules. It is by far the most widely used chromatographic medium. Since agarose contains more activatable hydroxyl groups, different ligands can be inserted under certain conditions as me...

Claims

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Application Information

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IPC IPC(8): C08L5/12C08K9/10C08K3/22C08J3/12B01J20/24B01J20/28H01F1/42
Inventor 任辉秦炜谭建雄
Owner BEAVERNANO TECH