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Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease

A technology of simultaneous processing and oblique-growing Scenedesmus, applied in biological water/sewage treatment, water/sludge/sewage treatment, microorganisms, etc., can solve the problem of poor decontamination ability, difficult to degrade carbon source substances, and low algal cell lipid content and other problems, to achieve the effect of strong anti-pollution ability and resource-based treatment

Active Publication Date: 2014-01-01
HARBIN INST OF TECH
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problems encountered in the coupling process of microalgae biodiesel production system and municipal sewage treatment system, such as poor stress resistance of algae species, low content of algae cell lipids, poor decontamination ability, difficulty in degrading carbon source substances in sewage and other problems. The problem of adding exogenous nutrients to growth in sewage provides a strain of Scenedesmus obliquus that simultaneously treats municipal sewage and realizes oil accumulation

Method used

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  • Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease
  • Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease
  • Scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease

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specific Embodiment approach 1

[0012] Embodiment 1: In this embodiment, a strain of Scenedesmus obliquus that synchronously treats municipal sewage and realizes oil accumulation is Scenedesmus obliquus PF3, preserved in the General Microorganism Center of China Microbial Cultures Preservation Management Committee, preserved The address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is July 30, 2013, and the preservation number is CGMCC No.8015.

[0013] In this embodiment, a strain of Scenedesmus obliquus that simultaneously treats municipal sewage and realizes oil accumulation, which is Scenedesmus obliquus PF3, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District, the preservation date is July 30, 2013, and the preservation number is CGMCC No.8015.

[0014] The Scenedesmus obliquus PF3 of the present e...

specific Embodiment approach 2

[0018] Specific embodiment 2: Scenedesmus obliquus PF3 in this embodiment is screened from the lakes of the Bund Wetland Park in the West District of Sun Island, Harbin, Heilongjiang. Filtering is done in the following steps:

[0019] (1) Carefully collect the planktonic microalgae in the water with a sterile beaker, and store the collected water samples in a transparent glass bottle filled with sterile medium, put the glass bottle into a thermos bottle with ice cubes, and then bring it into the laboratory Separation and purification were carried out within 24 hours.

[0020] (2) Take an appropriate amount of algae source and add it to the Erlenmeyer flask containing sterilized BG11 medium, add a small amount of glass beads at the same time, place it on a shaker and shake it for about 24 hours, take it out and let it stand for about 30 minutes, absorb 50mL of the middle liquid, add about 150mL Place the flask in a sterile BG11 culture medium in a constant temperature light in...

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Abstract

The invention relates to scenedesmus obliquus, in particular relates to scenedesmus obliquus capable of synchronously processing municipal sewage and accumulating grease, and aims at solving the problem in the coupling process of a microalgae biodiesel production system and a municipal sewage processing system that the algae has poor stress resistance, low content of cell fat, and poor decontamination ability, has difficulty in degrading carbon source substances in a water body and needs external source nutrient substances for growing in the water body. The scenedesmus obliquus PF3 is preserved in China general microbiological culture collection center (CGMCC) at No.3, No. 1 yard, Beichen west road, Chaoyang district of Beijing on July 30, 2013 with the preservation number of CGMCC No.8015. The scenedesmus obliquus has the advantages of having strong anti-pollution capacity, and being capable of growing and breeding with contaminants such as carbon, nitrogen and phosphorus in unsterilized general municipal sewage as nutrient substances, and accumulating grease.

Description

technical field [0001] The invention relates to Scenedesmus obliquus. Background technique [0002] Facing the increasingly severe challenges of fossil energy and environmental pollution, the use of microalgae to produce biodiesel has attracted more and more attention. This is because microalgae, as a raw material for biodiesel production, has high energy conversion efficiency, high oil content and fast proliferation. The advantages. In addition, the production process of microalgae biodiesel does not increase greenhouse gas emissions, which is conducive to environmental protection. However, the high production cost of microalgae biodiesel limits its large-scale production and wide application, and nutrients such as carbon, nitrogen, and phosphorus sources required for the growth of microalgae are an important part of the production cost of microalgae biodiesel. Municipal sewage is rich in carbon, nitrogen and phosphorus pollutants, and the massive discharge of these subst...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C02F3/34C12R1/89
Inventor 于艳玲冯玉杰张大伟仪超
Owner HARBIN INST OF TECH
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