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Preparation technology of lecithin-treated adipose stromal vascular components

A technology of stromal vascular components and lecithin, applied in the biological field, can solve the problems of stem cells losing stemness, having a great influence on cell activity, and losing stemness, etc., and achieving the effect of simple technology

Inactive Publication Date: 2014-01-01
FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After being cultured by the current conventional culture method, these sub-totipotent stem cells will differentiate into mature stem cells, and gradually lose their stemness and their superior therapeutic ability.
[0005] The main problems in the prior art methods: 1) Using digestive enzymes such as collagenase and trypsin to digest, which has a great impact on cell activity, etc.
2) These digestive enzymes need to use serum to terminate their activity (such as fetal bovine serum), which can easily cause some allergic reactions
3) The residual digestive enzymes in the obtained matrix components are not directly applicable to clinical treatment
4) After culture, more types of stem cells lose their stemness and superior therapeutic ability

Method used

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  • Preparation technology of lecithin-treated adipose stromal vascular components
  • Preparation technology of lecithin-treated adipose stromal vascular components
  • Preparation technology of lecithin-treated adipose stromal vascular components

Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Reagent source: Lecithin is a clinically approved drug, containing fat emulsion. Physiological saline is physiological saline for clinical use for injection.

[0033] 2. The experimental steps are as follows:

[0034] 1) Under local anesthesia with lidocaine, 20ml of adipose tissue was extracted from the abdomen with a sterile syringe;

[0035] 2) Add 20ml of 12 mg / mL lecithin and shake vigorously for 15 minutes;

[0036] 3) Filter through a 100-mesh filter under pressure to remove residual tissue;

[0037] 4) Collect the cell suspension, centrifuge at 1800rpm for 10 minutes, and discard the mature adipocytes in the supernatant;

[0038] 5) Add 50ml of normal saline, centrifuge and wash;

[0039] 6) Add 30ml of lecithin to resuspend the precipitated stromal vascular fraction;

[0040] 7) 200 mesh (75μm) filter;

[0041] 8) The collected filtrate is the stromal vascular fraction.

[0042] 9) The prepared stromal vascular components can be directly used for loca...

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Abstract

The invention discloses preparation technology of lecithin-treated adipose stromal vascular components, which comprises: extracting adipose tissues from abdomen, treating the adipose tissues through lecithin and centrifugally filtering to obtain the stromal vascular components. The technology disclosed by the invention is simple and feasible and can be used for more effectively obtaining stromal vascular fraction containing various types of stem cells from the adipose tissues, and meanwhile, effectively protecting the activity and functions of the stem cells of the components, and the technology can be directly and safely applied in clinic treatment.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a preparation technology of lecithin-treated adipose matrix blood vessel components suitable for clinical practice. Background technique [0002] Adipose stromal vascular fraction (SVF) refers to the stromal cells with stem cell characteristics obtained after removing mature adipocytes from adipose tissue, including adipocytes (MUSE CELL), mesenchymal stem cells (MSC), hematopoietic stem (progenitor ) cells (HSC), endothelial progenitor cells, lymphocytes, macrophages, etc., are a group of mixed cell populations. [0003] SVF can promote angiogenesis, prevent adipocyte apoptosis, improve the survival of adipose tissue by differentiating into endothelial cells or secreting pro-angiogenic factors such as VEGF, which is beneficial to autologous fat transplantation. Using SVF as a three-dimensional biological scaffold can restore the microenvironment of cell growth t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/0789C12N5/074
Inventor 谭建明陈津黄梁浒徐秀敏
Owner FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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