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High cellulase yield strain and method for producing neutral cellulase through fermentation

A neutral cellulase and cellulase technology, applied in the biological field, can solve the problems of high cost, small scale, and no wild strains with high enzyme-producing activity or genetically modified engineering bacteria technology.

Active Publication Date: 2014-01-15
湖南康捷生物科技有限公司
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AI Technical Summary

Problems solved by technology

Although my country has become the fourth country in the world that can produce cellulase after the United States, Japan, and Denmark, the production technology of neutral cellulase in my country is still relatively backward compared with developed countries, with low enzyme activity, high cost and small scale. , the root cause is that no wild strains with high enzyme activity have been screened or the technology of genetically modified engineered bacteria is still in the development stage

Method used

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  • High cellulase yield strain and method for producing neutral cellulase through fermentation
  • High cellulase yield strain and method for producing neutral cellulase through fermentation
  • High cellulase yield strain and method for producing neutral cellulase through fermentation

Examples

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Embodiment 1

[0072] Take a ring of slant strains and spot mycelium on a PDA plate, culture at 30°C for 7 days, take a plate and wash it with 10ml of sterile water, break it into a uniform spore suspension, take 1ml of the spore suspension and insert it into a Prepare several parts in a 250ml Erlenmeyer flask of 50ml seed culture medium. Culture conditions: rotation speed 200rpm, temperature 30 degrees, constant temperature culture for 3 days, which is fermented seed liquid.

[0073] Put 5L of enzyme-producing medium in a 7L fermenter, add 2.5ml of defoamer, and sterilize at 121°C for 30 minutes. After cooling, add 500ml of the above-mentioned fermented seed liquid according to 10% inoculum. The culture conditions are: speed 200r / min, temperature 30°C, ventilation rate 1.2L / min / L, after 150 hours of fermentation time, extract the crude enzyme liquid, and measure the enzyme activity according to the enzyme activity method, the enzyme activity of the fermentation liquid reaches 5080IU / L.

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Abstract

The present invention provides a high cellulase yield strain Trichodema reesei QZ-5, which is preserved in the China Center for Type Culture Collection on June 5, 2012, and has a preservation number of CCTCC NO:M2012204. The invention further discloses a method for producing neutral cellulase by adopting the strain as a starting strain, carrying out seed culture, adopting corn pulp, ammonium sulfate and the like as a nitrogen source, and adopting glucose, wheat bran and the like as a carbon source, wherein the highest enzyme activity of the fermentation broth can be more than 5000 IU / L, and the produced neutral cellulase can be widely used in fields of textile, papermaking, feeds, food and the like, and belongs to the technical field of biology.

Description

technical field [0001] The present invention utilizes a high-yield cellulase strain (Trichodema reesei QZ-5) and utilizes the strain to produce neutral cellulase through seed cultivation, corn steep liquor, ammonium sulfate, etc. as nitrogen sources, and glucose, bran, etc. as carbon sources. The cellulase method, the prepared enzyme can be applied to the fields of textile, papermaking, feed, food and the like, and belongs to the field of biotechnology. Background technique [0002] Cellulase is a kind of multi-component complex enzyme system that can degrade cellulose into glucose, which can be widely used in energy, textile, papermaking, feed, food and other fields. In recent years, with the increasing shortage of global resources and energy, Cellulase is the main enzyme system that uses the most widely distributed, most abundant, and cheapest cellulose in nature as a renewable resource, so the research on the production and preparation of cellulase has become a topic of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/885
Inventor 李洪兵张峰张明胡永明
Owner 湖南康捷生物科技有限公司
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