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Polypyrrole-F127 nano composite and application thereof in preparation of electrochemical sensor for whole blood detection

A nanocomposite material, F127 technology, applied in the field of electrochemical sensors, can solve the problems of biofouling on the surface of electrodes

Inactive Publication Date: 2014-01-22
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both metal nanoparticles and carbon nanotubes will exhibit non-specific adsorption behavior on fibrinogen, an important blood coagulation factor in plasma, resulting in biofouling on the electrode surface.

Method used

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  • Polypyrrole-F127 nano composite and application thereof in preparation of electrochemical sensor for whole blood detection
  • Polypyrrole-F127 nano composite and application thereof in preparation of electrochemical sensor for whole blood detection
  • Polypyrrole-F127 nano composite and application thereof in preparation of electrochemical sensor for whole blood detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] In Example 1, a polypyrrole-F127 nanocomposite material (50 μl of pyrrole reagent) was prepared.

[0025] Fully dissolve F127 (1.5g) in deionized water (30ml), and add the weighed FeCl 3 ·6H 2 O (0.9g), add pyrrole reagent (50μl) under rapid stirring, react at 0~5℃ for 6h, then transfer to dialysis bag (MWCO=14400) for dialysis to remove Fe 3+ , Fe 2+ And excess F127, finally a black polypyrrole-F127 nanocomposite material is obtained. See the electron microscope results figure 1 .

[0026] In addition, a polypyrrole material without F127 as a dispersant was prepared for comparison, see Figure 5 Wherein, bottle a is a polypyrrole material without F127 as a dispersant, bottle a is a polypyrrole material without F127 as a dispersant, and bottle b is a polypyrrole-F127 nanocomposite material. It can be seen from the figure that the polypyrrole nanoparticles without F127 precipitated to the bottom of the bottle, and the electrode could not be modified in the next step. The po...

Embodiment 2

[0027] In Example 2, a polypyrrole-F127 nanocomposite material (100 μl of pyrrole reagent) was prepared.

[0028] Fully dissolve F127 (1.5g) in deionized water (30ml) and add the weighed FeCl 3 ·6H 2 O (0.9g), add pyrrole reagent (100μl) under rapid stirring, react at 0~5℃ for 6h, then transfer to dialysis bag (MWCO=14400) for dialysis to remove Fe 3+ , Fe 2+ And excess F127, the black polypyrrole-F127 nanocomposite is finally obtained. See the electron microscope results figure 2 .

Embodiment 3

[0029] In Example 3, the blood compatibility of the polypyrrole-F127 nanocomposite was measured and modified on the electrode surface to design an electrochemical sensor that can directly detect the glucose concentration in whole blood.

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Abstract

The invention relates to a polypyrrole-F127 nano composite and application thereof, and the polypyrrole-F127 nano composite can be directly used in an electrochemical sensor for whole blood detection. The prepared polypyrrole-F127 nano composite having good blood compatibility is decorated onto the surface of an electrode, the new electrochemical sensor capable of directly detecting the glucose concentration in whole blood is designed, the application of the nano composite in biochemical analysis and a biosensor whole blood detection technique can be realized, the influence of common interfering substances in blood can be avoided by the method, the method has the advantages of wide detection range, good reproducibility, accurate results and the like, at the same time the whole blood detection can avoid blood processing and a long analysis time, and the method has a certain clinical application value.

Description

Technical field [0001] The invention relates to an electrochemical sensor that can be directly applied to whole blood detection. By preparing a polypyrrole-F127 nanocomposite with good blood compatibility and modifying it on the electrode surface, it designs a novel electrochemical sensor that can directly detect the concentration of glucose in whole blood, and realizes the biosensor whole blood detection Possible. technical background [0002] Normal blood chemical components, including protein, lipids, carbohydrates, non-protein nitrogen, inorganic salts, and other substances such as bilirubin, ketone bodies, enzymes, hormones and vitamins. The concentration of these components reflects the various metabolic conditions in the body. For a healthy body, various chemical substances have certain normal values. If it is outside the normal range, it increases or decreases, indicating the abnormal metabolism of a certain substance in the body, that is, a pathological state. Theref...

Claims

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Application Information

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IPC IPC(8): C08G73/06G01N27/327
Inventor 毛春孙冲葛晓维沈健陈丽彬马颖慧
Owner NANJING NORMAL UNIVERSITY