Drug-resistant glioblastoma cell line of primary temozolomide and bevacizumab, construction method and application of cell line

A technology of glioblastoma and bevacizumab, applied in the direction of microorganism-based methods, animal cells, tumor/cancer cells, etc., can solve the problems of not being able to guide the application well, and achieve stable and stable properties genetic effect
CN103525765BInactive Publication Date: 2014-12-17ζŽζ–‡ζ–Œ +2
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
ζŽζ–‡ζ–Œ
Publication Date
2014-12-17
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab, a construction method and application of the cell line. The construction method comprises the following steps: obtaining tissue specimens of patients with drug-resistant glioblastoma cell of primary temozolomide and bevacizumab through clinical screening, primarily culturing tumor cells through an enzyme digestion method, and continuously extending generation to 50 generations to obtain a strain of drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab; the microbial preservation number of the drug-tolerant glioblastoma cell line of primary temozolomide and bevacizumab is CGMCC No.8205. The glioblastoma cell line, disclosed by the invention, is stable in characteristics, stably heritable and high in tolerance to temozolomide and bevacizumab; the cell generation cycle lasts about 48 hours; the glioblastoma cell line has strong invasiveness and tumorigenicity. The glioblastoma cell line constructed by the construction method has important application prospect in aspects of screening medicines for treating glioblastoma and researching nosogenesis of the glioblastoma, and the like.
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Description

technical field

[0001] The present invention relates to a glioblastoma cell line, in particular to a primary temozolomide- and bevacizumab-resistant glioblastoma cell line and its construction method. The present invention further relates to the glioblastoma cell line The application of tumor cell lines in the screening of candidate drugs for temozolomide and bevacizumab-resistant glioblastoma, preparation of human glioblastoma models, etc. belongs to the field of construction of glioblastoma cell lines. Background technique

[0002] Glioma is the most common intracranial malignant tumor, accounting for about 35% to 61% of intracranial tumors. Because of its abnormal biological characteristics such as rich blood vessels, invasive growth, and lack of apoptosis, gliomas are highly invasive, and the existence of the blood-brain barrier has brought many difficulties to the treatment of gliomas. There are more than 15,000 new cases of malignant glioma in the United States each y...

Claims

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