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A method for rapid section staining of Miscanthus

A technology for section staining and Miscanthus, which is applied in plant section dyeing, Miscanthus fast section dyeing and used in the field of microscopic examination, which can solve the problems of affecting the degree of transparency, loss of scientific research work, affecting the quality of wax blocks, etc., and achieve high accuracy. and sensitivity, the production time is not long, the effect of shortening the detection time

Inactive Publication Date: 2017-06-30
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Paraffin film production is a continuous operation process, which often takes several days to complete. It is easy to conflict with other tasks, and it will also cause considerable losses to scientific research due to the reverse sequence or exceeding the specified time.
All the steps of film production are interrelated, if the dehydration is not complete, it will affect the degree of transparency, and even affect the quality of the wax block

Method used

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  • A method for rapid section staining of Miscanthus
  • A method for rapid section staining of Miscanthus
  • A method for rapid section staining of Miscanthus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take fresh 3-10cm stems of Miscanthus, soak them in 70% ethanol for 30 seconds, add 55% malonic acid aqueous solution to soften them for 40 minutes; -6 mol / L sodium hydroxide solution for 8 minutes, rinsed three times with distilled water, and then sliced ​​quickly with a handle scalpel to obtain a large number of thin slices of Miscanthus stems; rinsed the cut slices twice with distilled water, and added 1% tomato Red staining for 1 hour; after safranin staining, remove the staining solution and rinse with distilled water once, then soak in 30% ethanol, 50% ethanol, 70% ethanol, 85% ethanol, and 95% ethanol for 30 seconds; again stain with 0.5% fast green for 60 Seconds, soak in 95% ethanol for 30 seconds, then soak in 100% ethanol for 10 minutes; soak in ethanol xylene solution for 3 minutes, then soak in 100% xylene for 5 minutes, repeat once, take out the thin section and seal it with gum, and examine it under an ordinary optical microscope To observe the growth of ...

Embodiment 1

[0040]Take fresh 3-10cm stems of Miscanthus plants and put them in a 1:1 mixture of hydrogen peroxide and acetic acid (or 2:1), take out the samples with tweezers every 20 or 30 minutes to 1 hour, and rinse with running water After about 10 minutes, try cutting with a blade. If it feels soft, you can rinse it in water for a day and slice it. It can also be stored in a mixture of half and half glycerol-ethanol for later use; wash the cut slices twice with distilled water, add 1% safranin and stain for 1 hour; Soak in 30% ethanol, 50% ethanol, 70% ethanol, 85% ethanol, and 95% ethanol for 30 seconds; again stain with 0.5% fast green for 60 seconds, soak in 95% ethanol for 30 seconds, and then soak in 100% ethanol for 10 minutes; Soak in ethanol xylene solution for 3 minutes, then soak in 100% xylene for 5 minutes, repeat once, take out the thin section and seal it with gum, and observe the growth of vascular bundles under an ordinary optical microscope. The results are shown in ...

Embodiment 2

[0042] Take fresh 3-10cm stems of Miscanthus plants, soak them in 70% ethanol and 50% ethanol for 1 hour respectively, add 15% hydrofluoric acid aqueous solution for softening treatment for 10-15 days; take out the material, rinse it with distilled water three times, and then use Slice quickly with a handle scalpel to obtain a large number of thin slices of Miscanthus stems; wash the cut slices twice with distilled water, add 1% safranin and stain for 1 hour; Soak in 30% ethanol, 50% ethanol, 70% ethanol, 85% ethanol, and 95% ethanol for 30 seconds; again stain with 0.5% fast green for 60 seconds, soak in 95% ethanol for 30 seconds, and then soak in 100% ethanol for 10 minutes Soak in ethanol xylene solution for 3min, then soak in 100% xylene for 5min, repeat once, take out the thin section and seal it with gum, and observe the growth of vascular bundles under an ordinary optical microscope, see Figure 10-13 .

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Abstract

The invention belongs to the technical field of biological sectioning and relates to a method for sectioning and dyeing miscanthus plants. The method comprises the following steps: taking 3cm-10cm of a fresh miscanthus plant stem; immersing by alcohol and carrying out suitable softening treatment; sectioning and dyeing a sectioned thin sheet; fixing and de-coloring; then dyeing and flaking microscopic examination. According to the method, the softening degree is easy to master and the problems that the sheet is too hard or too soft and the like are solved; the sectioning integrity is easy to realize; the method is good for post-period dyeing and flaking.

Description

technical field [0001] The invention belongs to the technical field of biological slices, and relates to a method for dyeing plant slices, in particular to a method for quickly staining slices of Miscanthus plants for microscopic examination. Background technique [0002] Biomass is the third largest energy source in the world after coal and petroleum (Liang, 2000). Biomass resource reserves are very rich. Biomass energy provides 14% of the world's energy every year and becomes the source of dependence for about 1.5 billion people in the world. To survive as the main energy source (KOUFOPANOS, 1989). Today, the use of perennial herbaceous plants to develop the biomass energy industry is a new direction for the development of renewable energy. Research on lignocellulosic energy plants has received widespread attention in recent years. Cellulosic raw materials are the most abundant renewable resources on the earth. 35% to 50% of plant dry weight is cellulose, and 20% to 35% i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 覃静萍易自力
Owner HUNAN AGRICULTURAL UNIV
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