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Functional molecular markers of related genes of sweetness and sourness characters of muskmelon and application of markers

A molecular marker and functional technology, applied in the field of agricultural biology, can solve the problems of research and reporting of functional molecular markers of genes related to sweet and sour traits of melon, so as to improve selection accuracy, high efficiency, and shorten breeding cycle Effect

Active Publication Date: 2014-02-05
XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These molecular marker technologies developed in recent years are of great significance for transforming traditional phenotypic selection into direct selection of genotypes through molecular marker-assisted selection. Research and Reporting on Labeling

Method used

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  • Functional molecular markers of related genes of sweetness and sourness characters of muskmelon and application of markers
  • Functional molecular markers of related genes of sweetness and sourness characters of muskmelon and application of markers
  • Functional molecular markers of related genes of sweetness and sourness characters of muskmelon and application of markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Acquisition of functional molecular markers for genes related to sweet and sour taste traits of melon

[0063] Obtaining functional molecular markers of genes related to sweet and sour taste traits of melons includes the following steps:

[0064] (1) The high-sugar melon inbred line "Shouxing" was used as the female parent and the melon inbred line "Sour Melon" was used as the male parent to obtain the hybrid F 1 Namely "Flavor No. 4"; by Hybrid F 1 Self-pollination to obtain F 2 generation group, the parents, F 1 and each F 2 The sweet and sour taste of a single plant fruit was identified by instrumental measurement combined with tasting, and four types were obtained: only sour but not sweet, only sweet but not sour, both sour and sweet, and neither sour nor sweet.

[0065](2) Extract the genomic DNA of each material in (1) by CTAB method, 50 individual plants for 5 male parents, 5 female parents, only sour but not sweet, only sweet but not sour, neith...

Embodiment 2

[0068] Example 2: Super-BSA based on simplified genome sequencing (SLAF-Seq)

[0069] (1) Extract 5 male parents, 5 female parents, F 2 Genomic DNA from each individual plant of 50 individual plants with only sweet but not sour, only sour but not sweet, and neither sour nor sweet, were mixed in equal amounts to form 5 mixed pools.

[0070] (2) SLAF -seq library construction and sequencing.

[0071] The first is to simplify the design and genomic DNA digestion: use the enzyme digestion prediction software to analyze the GC content, repeat sequence and gene characteristics of the five genomic DNA pools in step (1), and use XhoI+ MseI endonuclease to digest the mixed genomic DNA. Cut the DNA into 450-500bp fragments; the enzyme digestion system is 500ng of genomic DNA, 41L of buffer from New England Biolabs, 0.12L each of XhoI and MseI, supplemented with ddH 2 From 0 to 50 L, mix the reagents evenly after preparation, and incubate at 37°C for 15 hours, and purify with a kit...

Embodiment 3

[0080] Example 3: Mining of genes related to sweetness and sourness traits of melon

[0081] (1) Through instrumental measurement of fruit flavor combined with tasting identification, the female parent is only sweet but not sour, the male parent is only sour but not sweet, F 1 For both sour and sweet, F 2 In the population, there were 130 plants that were sweet but not sour, 77 plants that were only sour but not sweet, 60 plants that were neither sour nor sweet, and 233 plants that were both sour and sweet. Among them, sweet:not sweet=2.65:1, sour:not sour=1.63: 1. Combining parental traits and F 1 Traits and F 2 The segregation ratio of traits, sweetness and sourness are both dominant traits, sweetness is biased towards quality traits, sourness is biased towards quantitative traits, and a cross model with AAbbxaaBB as the parent is designed.

[0082] (2) Use the ratio correlation method to locate the sweetness and sourness traits. Select the pure sum mark with a / b>3 or...

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Abstract

The invention discloses functional molecular markers of related genes of sweetness and sourness characters of muskmelon and applications of the markers. According to the markers and the applications of the markers, by utilizing a Super-BAS technique, the functional molecular markers of the related genes of the sweetness and sourness characters of the muskmelon are provided; related gene sequences of the sweetness and sourness characters of flavor muskmelons are confirmed; a functional molecular marker of the related gene of the sweetness character, namely SLAF18745-S01 and three functional molecular markers of the related gene of the sourness character, namely SLAF36334-S02, SLAF50072-S03 and SLAF31212-S04 are successfully developed on the basis; conventional phenotypic selection is changed into direct genotypic selection through sweetness and sourness marker assisted selection, so that the functional molecular markers have the important application value and significance on the improvement of the sweetness and sourness characters of the muskmelons.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology. Specifically, the invention relates to the technical field of functional molecular markers of genes related to the sweetness and sourness of melon fruit and their application. Background technique [0002] melon( Cucumis melo L. ) belongs to Cucurbitaceae Melon, and is an important horticultural crop. The planting area and output of muskmelon in my country rank first in the world. Melon is a highly polymorphic species containing a variety of genotypes with different fruit flavors. The sugar and acid components and their content of ripe melon fruit have an important influence on the internal quality of the fruit, and are important indicators to determine the flavor of the fruit. The change of sweet, sour and other flavors is a complicated process, which is affected and regulated by a series of related genes. Locate genes related to sweetness and sourness traits of melon and develop fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/10C12Q1/68
Inventor 张红吴明珠张永兵张学军吴海波伊鸿平
Owner XINJIANG AGRI SCI ACAD CANTALOUPE RES CENT
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