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A method for producing ethanol by enzymatic hydrolysis, saccharification and fermentation of grass raw materials

A technology of saccharification and fermentation and raw materials, which is applied in the field of bioethanol production, can solve the problems of low ethanol concentration in fermentation broth, high energy consumption, and high dosage of cellulase, and achieve the effects of reducing pollution, reducing dosage, and reducing dosage

Active Publication Date: 2016-02-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the deficiencies of the prior art, the problem to be solved by the present invention is to provide a grass-based product that solves the problems of high cellulase usage, high energy consumption, and low ethanol concentration in fermentation broth that often occur in the process of bio-transformation of grass raw materials to produce bioethanol. Method for producing bioethanol by hydrolysis, saccharification and fermentation of cellulose as raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Use a lawn cutter to cut corn stalks into 3-7cm lengths and put them into a high-pressure container. Add water according to the ratio of absolute dry raw material weight to water volume, that is, the ratio of kg:liter to 1:15, and then heat up. To 180 ℃, carry out hot water treatment 20min; The material after hot water treatment is carried out solid-liquid separation, and the solid material separated is transferred in the normal pressure container, according to the ratio of material weight and surfactant volume, namely kilogram: liter is The solid-liquid ratio of 1:15 was directly added to the surfactant Tween 40 solution with a concentration of 1.5%, and treated at a temperature of 25°C for 60 minutes; then, the material after the surfactant treatment was subjected to solid-liquid separation, and the separated surface-active The liquid recirculation of active agent is used for above-mentioned surfactant treatment step, and the separated solid material is transferred in ...

Embodiment 2

[0022] Use a lawn cutter to cut the reed into a length of 3 to 7 cm and put it into a high-pressure container. Add water according to the ratio of absolute dry raw material weight to water volume, that is, the ratio of kilogram:liter to 1:8, and then heat up to 1:8. 220°C, carry out hot water treatment for 30min; carry out solid-liquid separation of the material after hot water treatment, and the separated solid material is transferred to the normal pressure container, according to the ratio of material weight to surfactant volume, that is, kilogram: liter is 1 The solid-liquid ratio of :6 directly adds the surfactant Tween 80 solution that concentration is 2.0%, handles 90min at temperature 0 ℃; The liquid recirculation of agent is used for above-mentioned surfactant treatment step, and the separated solid material is transferred in the fermenter, then adds cellulase in the amount of 20FPU / g material in solid material, and adjusts material concentration weight ratio to be 20 ...

Embodiment 3

[0025] Use a lawn cutter to cut the corncobs into 3-5cm lengths and put them into a high-pressure container. Add water according to the ratio of absolute dry raw material weight to water volume, that is, the ratio of kg:liter to 1:20, and then heat up. To 250 ℃, carry out hot water treatment 10min; The material after hot water treatment is carried out solid-liquid separation, and the separated solid material is transferred in the normal pressure container, according to the ratio of material weight and surfactant volume, namely kilogram: liter is The solid-liquid ratio of 1:20 was directly added to the surfactant Tween 60 solution with a concentration of 0.01%, and treated at a temperature of 80°C for 120 minutes; then, the material after the surfactant treatment was subjected to solid-liquid separation, and the separated surface-active agent contained The liquid recirculation of active agent is used for above-mentioned surfactant treatment step, and the separated solid material...

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PUM

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Abstract

The invention discloses a method for producing ethanol through enzymolysis saccharification and fermentation of a grass raw material. The method comprises the following steps of firstly cutting the raw material into certain length, putting into a high-pressure container, adding water and heating for hot water treatment; after the hot water treatment, performing solid-liquid separation; without water washing, directly adding the solid material into a surfactant with concentration of 0.01-3%, and performing pretreatment at normal temperature; after treating the surfactant, performing solid-liquid separation; circularly treating the separated surfactant liquid by the surfactant treatment step; transferring the separated solid material into a fermentation pot, and sequentially adding cellulase and yeast; performing supplement semi-simultaneous saccharification and fermentation to produce ethanol. By adopting the method disclosed by the invention, the pretreated material can be directly applied to fermentation without needing washing; the method has the advantages that the washing water and energy consumption can be remarkably saved, the dosage of cellulase is low, the surfactant is recyclable and the like.

Description

technical field [0001] The invention relates to a method for producing bioethanol, in particular to a method for producing bioethanol by using grasses as raw materials through cellulase hydrolysis, saccharification and fermentation. Background technique [0002] Grass raw materials are cheap, easy-to-obtain, and abundant renewable resources. Through biotransformation, they can be used to produce liquid fuels and bulk chemicals needed by humans to partially replace non-renewable resources such as petroleum. It is of great significance to reduce the emissions of fossil fuels such as oil and coal, etc. Biotransformation of grass raw materials to produce fuel ethanol generally requires three main steps: pretreatment, cellulase hydrolysis, and ethanol fermentation. Among them, using cellulase to efficiently hydrolyze grass raw materials to produce fermentable sugars an important step. However, due to the dense structure and complex chemical composition of grass raw materials, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/10
Inventor 赵建李雪芝鲁杰
Owner SHANDONG UNIV