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Method for extracting protein of fly maggot through ceramic membrane

A technology of fly maggot protein and ceramic membrane, which is applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of low purity and low filtration precision, and achieve high extraction rate, simple process and simple operation Effect

Active Publication Date: 2014-02-05
JIANGSU JIUWU HITECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the protein extracted by the above-mentioned method retains its biological activity, it is all filtered with a filter cloth, and the filtration accuracy is not high. The finished product contains many invalid impurity components, so the purity is not high. Extract protein, but not all proteins can be absorbed by the human body, so it is necessary to develop a method for extracting proteins with high purity and easy absorption by the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The fly maggots were washed, dried and ground into powder. After 1kg of fly maggot powder and 20kg of water were mixed uniformly, the temperature was raised to 50° C., 50g of trypsin was added for hydrolysis, and the hydrolysis was carried out for 60 minutes. The feed liquid entered the enzyme killing device. The temperature was raised to 75°C to inactivate the enzyme for 5 minutes. The hydrolyzate is sent to the sand filter for filtration, and the filtrate is sent to the ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 500 nm, the material is alumina, and the pH of the feed liquid is adjusted to about 6.0. The microfiltration process spans The membrane pressure difference was 0.05Mpa, the membrane surface flow rate was 1 m / s, and the filtration temperature was 40°C. After the feed liquid was concentrated 6 times, the microfiltration was stopped. The microfiltration permeate was sent to the ultrafiltr...

Embodiment 2

[0030]The fly maggots were washed, dried and ground into powder. After 1kg of fly maggot powder and 20kg of water were mixed uniformly, the temperature was raised to 50° C., 50g of trypsin was added for hydrolysis, and the hydrolysis was carried out for 60 minutes. The feed liquid entered the enzyme killing device. The temperature was raised to 75°C to inactivate the enzyme for 5 minutes. The hydrolyzate is sent to a sand filter for filtration, and the filtrate is sent to a ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 200 nm, the material is alumina, and the pH of the feed liquid is adjusted to about 6.0. The microfiltration process spans The membrane pressure difference was 0.1Mpa, the membrane surface flow rate was 1m / s, and the filtration temperature was 40°C. After the feed liquid was concentrated 6 times, the microfiltration was stopped. The microfiltration permeate is sent to the ultrafiltration me...

Embodiment 3

[0033] The fly maggots were washed, dried, and ground into powder. After 1kg of fly maggot powder and 30kg of water were mixed uniformly, the temperature was raised to 50° C., 50g of trypsin was added for hydrolysis, and the hydrolysis was carried out for 55 minutes. The feed liquid entered the enzyme killing device. The temperature was raised to 75°C to inactivate the enzyme for 5 minutes. The hydrolyzate is sent to a sand filter for filtration, and the filtrate is sent to a ceramic microfiltration membrane for microfiltration. The average pore size of the ceramic microfiltration membrane is 200 nm, the material is alumina, and the pH of the feed liquid is adjusted to about 6.0. The microfiltration process spans The membrane pressure difference was 0.3Mpa, the membrane surface flow rate was 5 m / s, and the filtration temperature was 40°C. After the feed liquid was concentrated 8 times, the microfiltration was stopped. The permeate of the microfiltration was sent to the ultrafi...

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Abstract

The invention relates to a method for extracting animal protein of fly maggot through a ceramic membrane separation technology. The method comprises steps of carrying out cleaning, crushing, thermal treatment, enzymolysis and enzyme deactivation on fresh fly maggot, isolating and purifying enzymatic hydrolysate through the ceramic membrane separation technology, then concentrating and spray-drying, removing large particulate matters in the enzymatic hydrolysate through a primary filter, then removing macromolecular colloid and oil in the enzymatic hydrolysate respectively through a ceramic micro filtration membrane and an ultrafiltration membrane, separating the decomposed protein from polypeptide and amino acid, spray-drying concentrated solution to obtain protein powder, concentrating through nanofiltration and drying to obtain a mixture of polypeptide, amino acid and micromolecular protein. According to the method, protein of fly maggot can be effectively obtained, the bitterness of the extracted product can be lightened, the extraction efficiency is high, and polypeptide, amino acid and micromolecular protein can be isolated.

Description

[0001] technical field [0002] The invention relates to a method for extracting protein from fly maggots, in particular to a process for extracting fly maggot animal protein by coupling enzymatic hydrolysis and a ceramic membrane method, and belongs to the technical field of biotechnology enzymatic hydrolysis of fly maggot protein and membrane separation. [0003] Background technique [0004] With the rapid development of the breeding and feed industries, my country's demand for animal protein is also increasing. At present, the main source of animal protein in my country is fishmeal. Domestic fishmeal cannot meet the market demand, so a large amount of fishmeal needs to be imported every year. The dry base of fly maggots contains 54%~65% of crude protein, 10%~14% of fat, 8%~10% of chitin and rich vitamins, trace elements, etc. The nutritional components are relatively comprehensive, and the total amount of amino acids is 2.3 times that of fish meal. , Methionine and lys...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 彭文博张桂花寇琴熊福军张宏范克银
Owner JIANGSU JIUWU HITECH
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