Core-shell structure electroactive composite fibers and preparation method of tissue engineering scaffold

Abstract

The invention discloses a preparation method of an electroactive micron / nanometer fiber scaffold which takes a degradable polymer as a shell and a conductive polymer as a core, has a core-shell structure and is formed by interlacing of composite fibers. The preparation method comprises the steps of preparing core spraying liquid; preparing shell spinning liquid; preparing conductive high-polymer / degradable polymer composite fibers with an electroactive core-shell structure and a composite fiber dual-pore structure scaffold by using a coaxial co-spinning device and combining a spinning technology with a spraying technology; cleaning and drying to obtain the scaffold. The electroactive micron / nanometer fiber scaffold prepared by the invention has stable conductivity and good biocompatibility required by electro-stimulation on cells, and well avoids direct contact between the conductive high-polymer and a cell culture solution or an organism at the same time, so that the problem of instable conductivity caused by de-doping is solved, and the scaffold has a very good practical value in the aspect of nerve tissue engineering.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to an electrically active composite fiber with a core-shell structure, which uses a degradable polymer as the shell and a conductive polymer as the core, and an electroactive composite micro / nano fiber interwoven therewith. Preparation method of fibrous scaffold. Background technique [0002] In vitro and in vivo experiments have shown that electrically active biomaterials can stimulate the regeneration of bone cells and nerve cells (Biomaterials 2001; 22: 1055). So far, polypyrrole, polyaniline and other conductive polymers are widely studied as electroactive biomaterials that can be used as substrates. In particular, polypyrrole has shown potential in biomedical applications. Its unique electrical activity, conductivity and biocompatibility to mammalian cells make it an ideal guide for nerve repair and guided nerve regeneration. Channel materials (J. Biomed. Mater. Res. A2000, 50:5...

AI Technical Summary

Problems solved by technology

However, the conductive polymer and degradable polymer material prepared by this method are prone to serious phase separation between the conductive component and the polymer material under the condition of ensuring high conductivity.

At the same time, in the process of scaffolds being cultured in vitro and implanted in vivo to repair damaged nerves, the current intensity passing through the cell culture scaffolds decreases due to the increase in the resistance of the scaffolds due to dedoping, and the decline is particularly severe at the initial stage, giving It is very difficult to stabilize the current

At present, there is no relevant research on the use of coaxial co-spinning device to combine electrospinning technology and electrostatic spraying technology to prepare a composite fiber scaffold material with a core-shell structure with a conductive polymer as the core and a degradable polymer material as the shell. to report

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