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Preparation method of southern Anhui Umbilicaria esculenta polysaccharide

A technology of polysaccharide and stone fungus, which is applied in the field of preparation of stone fungus polysaccharides in southern Anhui, can solve the problems of complex structure, difficulty in extraction, separation and purification, and achieve the effect of simple equipment, small investment and low cost

Active Publication Date: 2014-02-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly contains more than ten kinds of polysaccharides and other substances, the structure is complex, and it is difficult to extract, separate and purify

Method used

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  • Preparation method of southern Anhui Umbilicaria esculenta polysaccharide

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preparation example Construction

[0032] The preparation process method of the polysaccharide of the southern Anhui clamshell polysaccharide of the present invention comprises the following steps:

[0033] (1) Dried and pulverized the southern Anhui real estate stone fungus;

[0034] (2) Extract the crushed Auricularia with hot water, the leaching temperature is 60-90°C, the ratio of Auricularia to water is 1:5-40 (preferably 1:10-35), and each extraction time for 2 to 3 hours, after filtration, the filter residue was extracted again, a total of 3 to 4 extractions were performed, and the extraction liquid was combined;

[0035] (3) After the extraction solution is filtered and cooled, it is input into the membrane separation system 1 through a pressure pump at a working pressure of 0.01 to 0.2 Mpa. The membrane separation retentate contains cellulose, macromolecular proteins and pigments, etc.; Monosaccharides, polysaccharides, fats, amino acids, small molecular proteins and pigments, etc.; the macromolecular...

Embodiment 1

[0047] 1. Take 1000g of Anhui Shifu, dry it for about 3 days, and crush it with a crusher.

[0048] 2. Add 10,000g of water, heat to 80°C and soak for 2.5 hours, then filter with a 200-mesh filter, extract the filter residue again, extract 3 times in total, and combine the extracts.

[0049] 3. The extract solution is cooled to normal temperature, and is fed into a membrane separation system with a membrane molecular weight of 15,000 Daltons through a pressure pump at a working pressure of 0.2Mpa.

[0050] The membrane separation retentate contains cellulose, macromolecular proteins and pigments, etc.; the membrane separation permeate contains monosaccharides, polysaccharides, fats, small molecular amino acids, small molecular proteins and pigments.

[0051] 4. The permeate is fed into a membrane separation system with a membrane molecular weight of 6,000 Daltons through a pressure pump at a working pressure of 0.2Mpa.

[0052] Membrane separation intercepts the agaric polysa...

Embodiment 2

[0057] 1. Take 1000g of Wannan stone fungus, dry it for about 3 days, and crush it with a crusher;

[0058] 2. Add 20,000g of water, heat to 90°C and soak for 3 hours, filter with a 220-mesh filter, extract the filter residue again, extract 4 times in total, and combine the extracts;

[0059] 3. Cool the extract solution to normal temperature, and enter the membrane separation system with a membrane molecular weight of 15,000 Daltons through a pressure pump at a working pressure of 0.15Mpa. The membrane separation retentate contains cellulose, macromolecular proteins, and pigments; The liquid contains monosaccharides, polysaccharides, fats, small molecular amino acids, small molecular proteins and pigments;

[0060] 4. The permeate enters the membrane separation system with a membrane molecular weight of 6000 Daltons through a pressure pump at a working pressure of 0.15Mpa. The membrane separation intercepts and concentrates the agaric polysaccharides containing monosaccharide...

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Abstract

The invention discloses a preparation method of southern Anhui Umbilicaria esculenta polysaccharide, which takes southern Anhui Umbilicaria esculenta as a raw material. The preparation method comprises the following steps: crushing Umbilicaria esculenta, extracting for 3-4 times by hot water, wherein extraction is carried out for 2-3 hours each time, merging a leaching liquor, performing membrane separation to the leaching liquor to obtain a crude Umbilicaria esculenta polysaccharide permeation liquid, performing membrane separation on the permeation liquid to obtain a crude Umbilicaria esculenta polysaccharide trapped fluid, performing macroporous resin column chromatography on the trapped fluid, concentrating an eluate and performing alcohol precipitation to obtain the polysaccharide product, wherein the final purity can reach 92.5%. The whole set of production process enables continuous operation, and has the advantages of low energy consumption, mild condition, no destroy on effective components, high security and high yield of Umbilicaria esculenta polysaccharide. Resin enables regeneration and utilization; the solvent is recoverable and reusable, and has the advantages of environment-friendliness, raw material saving and cost reduction. The preparation method provides a technical base for exploiting southern Anhui Umbilicaria esculenta polysaccharide as medicinal resource and health food.

Description

technical field [0001] The invention relates to a method for preparing polysaccharides from southern Anhui clam, belonging to the fields of food, agricultural product processing and bioengineering. Background technique [0002] Stone ear (Umbilicaria esculenta or Umbilicaria hoffm) is the fruiting body of Umbilicariaceae (Umbilicariaceae, also known as Umbilicariaceae), a symbiont of algae and fungi, born on rocks, flat and irregularly round, with a Brown with black down on the back. Mainly distributed in Anhui, Zhejiang, Jiangxi and other places, it has the effects of clearing heat and detoxifying, quenching thirst and eliminating phlegm, relieving asthma and reducing inflammation, improving eyesight and improving essence, etc., and can also be used as medicine. Stone ear contains a lot of polysaccharides, colloid, iron, phosphorus, calcium, multivitamins, stone ear acid and pink rock acid. Pharmacological experiments have shown that polysaccharides of Auricularia can sig...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 王昱沣陆兆新颜智勇邵江娟张生生王慧琴陈阳阳
Owner NANJING AGRICULTURAL UNIVERSITY
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