Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of biological antibacterial agent

A biological antibacterial agent, biological technology, applied in the field of antibacterial agents, to achieve the effect of enhancing disease resistance

Inactive Publication Date: 2016-05-25
ZHANJIANG NORMAL UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Up to now, there is no concrete method about the use of immune induction technology to prepare biological antibacterial agents directly for agricultural antibiotic prevention by using live fifth instar silkworm larvae as raw materials and subcutaneously inoculating biologically induced strains at the silk glands in the middle of the silkworm body. to report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 The biological antibacterial agent of this example was prepared by the following steps:

[0034] (1) Preparation of biologically active non-pathogenic bacteria antibody liquid of Ralstonia solanacearum: at room temperature and 75% humidity, detoxify the fifth-instar silkworm by subcutaneous injection and inoculation at the 1 / 2 silk gland in the middle of the live body of Ralstonia solanacearum. 10 8 0.01mL of cfu / ml bacterial suspension, after 48 hours of in vivo immunization of silkworms, the cultured silkworms were pulverized with a tissue pulverizer, cultured for 72 hours under shading with magnetic stirring, and heated to 100°C for 10 minutes. Filter, and let the filtrate stand for 12 hours to obtain the biologically active non-pathogenic bacteria antibody liquid of Ralstonia solanacearum;

[0035] (2) Preparation of biologically active non-pathogenic bacteria antibody liquid of Phytophthora capsici: at room temperature and humidity of 80%, detoxify the fifth-i...

Embodiment 2

[0044] Example 2 The following steps are used to prepare the biological antibacterial agent of this example:

[0045] (1) The spore suspension of mixed source of bacteria and fungi adopts the following steps: the collected bacterial suspensions of Ralstonia solanacearum and Bacillus subtilis are first diluted with 0.5% physiological saline prepared with sterile water to Observe 45 spores in a field of view under a microscope, then assemble the separately diluted bacterial suspensions and mix them evenly, with a bacterial concentration of 10 4 cfu / ml, stored in a refrigerator at 4℃; the collected spore suspensions of Phytophthora capsici and Cucumber Fusarium wilt were diluted with 0.5% saline prepared with sterile water to observe a field of view under a microscope. Spores, and then assemble the diluted spore suspensions together, mix evenly, and have a bacterial concentration of 10 8 cfu.g -1 , Stored in a refrigerator at 4°C; finally, reassemble the mixed bacterial suspension an...

Embodiment 3

[0047] Example 3 The following steps were used to prepare the biological antibacterial agent of this example:

[0048] (1) The spore suspension of mixed source of bacteria and fungi adopts the following steps: the collected bacterial suspensions of Ralstonia solanacearum and Bacillus subtilis are first diluted with 0.5% physiological saline prepared with sterile water to Observe 50 spores in a field of view under a microscope, then assemble the diluted bacterial suspensions together, mix them evenly, and the bacterial concentration is 10 4 cfu / ml, stored in a refrigerator at 4℃; the collected spore suspensions of Phytophthora capsici and Cucumber Fusarium wilt are respectively diluted with 0.5% saline prepared with sterile water to observe a field of view under a microscope. Spores, and then assemble the diluted spore suspensions together, mix evenly, and have a bacterial concentration of 10 8 cfu.g -1 , Stored in a refrigerator at 4°C; finally, reassemble the mixed bacterial susp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a biological antibacterial agent. The preparation method comprises the following steps: respectively inoculating biological antibody-induced source bacterial strains to a fifth-instar larva living body and carrying out immune culture to prepare bioactive non-pathogenic bacterium antibody bacterial liquids; combining the bioactive non-pathogenic bacterium antibody bacterial liquids, drying, adding biological hormone, magnetic stirring and culturing, thereby obtaining the biological antibacterial agent; or inoculating a bacterial strain of hybrid source inoculum spore suspension liquid of bacteria and funguses obtained by biological antibody-induced source bacterial strains on the fifth-instar larva living body and carrying out antibacterial culture; then, culturing with biological hormone or cytokinin. The prepared antibacterial agent product can be used for preventing bacterial wilt and fusarium wilt for crops, and has an incidence prevention rate of 80%-90%. The product has a stronger antibacterial effect and efficient and broad-spectrum antibacterial advantages, and is subjected to a pilot test. The method disclosed by the invention is simple and convenient in technical route, stable in product, low in production cost, high in yield controllable degree, free from pollution, easy for result transformation and convenient for industrial production.

Description

Technical field [0001] The invention relates to an antibacterial agent, specifically a biological antibacterial agent. Background technique [0002] Antimicrobial (antimicrobialpeptide) or biological antibacterial agent is a kind of active protein polypeptide substance produced by the biological immune system to resist external biological infection sources, and it is widely present in insects, plants, animals and humans. In addition to non-specific anti-bacterial, fungal, virus, anti-infection, and anti-disease effects, it also has anti-cell mutation and anti-tumor cell effects, and does not damage normal human cells, especially for multi-drug resistant strains. Biological antibacterial agents have a strong killing effect on more than 40 kinds of Gram-positive and negative bacteria, Pseudomonas aeruginosa and paratyphoid bacteria. Effective biological resistance drugs are the focus and hotspot of research in the world today. Tomato and pepper bacterial wilt caused by bacterial w...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P1/00A01P3/00
Inventor 罗富英李典
Owner ZHANJIANG NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com