Cotton endophytic fungus cef-818 and its application in the control of cotton Verticillium wilt
A technology of CEF-818 and cotton verticillium wilt, which is applied to the endophytic fungus CEF-818 and its application field in the control of cotton verticillium wilt, can solve the problems of poor effect of cotton verticillium wilt and achieve improved control effect, Improved disease resistance and easy usage
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Embodiment 1
[0021] The isolation of embodiment 1 bacterial strain
[0022] (1) Sample collection
[0023] From June to August, healthy cotton plants were selected, and the middle and upper parts of the main stem and fruiting branches were taken 15 cm.
[0024] (2) Isolation of strains
[0025] Firstly, the collected samples (cotton stalks) were washed with clean water, peeled, placed in a sterilized petri dish with a diameter of 9 cm, and sterilized with 75% alcohol for 1 min. Then disinfect with 5.0% sodium hypochlorite for 60 seconds, and wash with sterilized water for 3 times, each time not less than 3 minutes. Then use sterilized absorbent paper to dry the water on the surface, cut into 0.4cm long pieces with sterilized scissors, place them in a 9cm petri dish, and incubate at 25°C. Use the last cleaning solution as a control to check whether the disinfection is thorough. Check the growth of endophytic fungi every day, and find that there are colonies growing out, and transfer the...
Embodiment 2
[0034] The inhibitory effect of embodiment 2CEF-818 on Verticillium dahliae of cotton
[0035] 1. Test method
[0036] Buckle cultivation method. Pour a certain amount of PDA medium into the lid and bottom of the 90mm-diameter Petri dish respectively, and the culture medium in the lid is slightly less. After solidification, add 5 μl of Penicillium CEF-818 bacteria solution in the center of the dish cover (Chapic liquid culture for 7 days), and inoculate 5 μl of Verticillium wilt pathogen bacteria solution in the center of the dish bottom (Chapic liquid culture for 7 days). Put them into a constant temperature incubator at 25°C, and culture them upside down. Take the petri dish inoculated only with pathogenic bacteria as a control, and repeat each treatment 4 times. The growth of pathogenic bacteria and Penicillium hyphae were observed every day, and the colony diameter of pathogenic bacteria was measured by cross method 7 days after inoculation with Verticillium dahliae, and...
Embodiment 3
[0041] The control effect of embodiment 3CEF-818 to cotton verticillium wilt
[0042] 1. Test method
[0043] CEF-818 was cultured in Chapic liquid medium at 25°C and shaken in the dark for 7 days, filtered through filter paper, and the culture filtrate was obtained for seed soaking. The control strain Vd080 (strong pathogenic strain of Verticillium dahliae) was cultured in Chapic liquid medium at 25°C in the dark for 7 days with shaking, filtered through three layers of gauze, and the spore suspension was obtained for inoculation. Four treatments were set up in the experiment, respectively 25%, 50% and 100% CEF-818 culture filtrate and clear water soaking seeds. All four treatments were inoculated with the conidia suspension of Verticillium dahliae Vd080 when a true leaf of cotton seedlings was flattened.
[0044] Mix the sterilized vermiculite and sand at a ratio of 6:4 to make a bottomless paper pot with a diameter of 6 cm, and place it in a plastic tray of 45 cm × 35 cm. ...
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