Preparation method for aseptic explants of gelidiella acerosa

Abstract

The invention discloses a preparation method for aseptic explants of gelidiella acerosa. The preparation method comprises the following steps: thalluses of gelidiella acerosa are used as explants, and placed in a mixed preparation of broad-spectrum antibiotic and eumycin for soaking for 15-18 h after being washed out by sterile filtered water; the explants of gelidiella acerosa are taken out and then are placed in a 1-3% potassium iodide solution for soaking for 2-5 min; the gelidiella acerosa is taken out, placed in a 3-10% sodium hypochlorite solution for soaking for 3-10 min, and washed out by the sterile filtered water, so that the aseptic red alga explants can be. According to the invention, the broad-spectrum antibiotic, antifungal agent, potassium iodide and sodium hypochlorite are cooperatively used as the antiseptics, so that bacteria, funguses and viruses on the surfaces of explants of gelidiella acerosa can be effectively killed, and the growth of plants cannot be affected, and 90% of aseptic available explants can be obtained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for preparing aseptic explants of cauliflower. Background technique [0002] Cauliflower ( Gelidiella acerosa (Forsskal)) is one of the important raw materials for the industrial production of agar products. The agar gel yield of cauliflower is very high, and the output of the fronds per square centimeter can reach 600g. Such a high agar yield makes people prefer to use cauliflower as a raw material for extracting agar. Every year, 200-300 tons of dried cauliflower materials harvested from natural populations are used for local agar production. Due to the rise of the biotechnology industry in the country, the demand for agar is increasing, resulting in the use of other agar seaweeds, and also in order to meet the needs of agar. Over-harvesting due to growing market demand for cauliflower. Efforts to restore and protect the natural algae beds of overexplo...

AI Technical Summary

Problems solved by technology

[0003] Compared with higher terrestrial plant tissue culture technology, the research on marine plant tissue culture technology is relatively backward. The biggest problem facing seaweed tissue culture is that it is impossible to obtain sterile and viable seaweed explants, because marine plants are composed of single or multi-layer It is composed of cells, and its organizational structure is very different from that of terrestrial plants. It does not have the vascular structure of stems and leaves of higher plants, nor does it have hard epidermis protection; disinfectants such as alcohol and mercury chloride commonly used in terrestrial plants are very Easily invades the interior of algae cells and causes explant death

At present, the tissue culture technology of macroalgae is relatively backward. According to the known data, antibiotic soaking or detergent cleaning are commonly used to achieve the purpose of disinfection and sterilization. However, detergent disinfection and cleaning are very difficult problems, and antibiotics have many hazards. However, it is not known what kind of harm to the treated explants and regenerated plants. The feasibility of the above-mentioned treatment of the explants of condensed cauliflower is very low, and only 50% of the sterile explants can be obtained at most.