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Polypeptide-zinc metal complex as well as preparation method and application thereof

A complex, zinc metal technology, applied in the field of metal polypeptide complex, polypeptide-zinc metal complex and its preparation field, can solve the problems of high cost, difficult chemical synthesis, low biological activity, etc., achieve low price, enhance immunity of the body effect of ability

Inactive Publication Date: 2014-03-26
天津拓飞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the chemical synthesis of pure peptides is difficult and costly, and the biological activity of chemically synthesized peptides is often lower than that of peptides prepared from animals and plants, the application of chemically synthesized peptides and trace elements to prepare metal complexes will also be affected. certain limit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing and chop them quickly with a tissue grinder or chopping machine to avoid the raw materials from forming a gel.

[0063] Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1:3, dissolve in water, stir, adjust the pH to 9.0, add alkaline protease 700U / g raw materials, and perform enzymatic hydrolysis reaction in a 50°C constant temperature water bath shaking shaker for 2 hours , shaking once every 10 minutes. Then heat the hydrolyzate to 100°C, keep it for 10 minutes, cool to room temperature, and filter to obtain the clear solution for later use. The alkaline protease used in the hydrolysis was Alcalase 2.4L FG, provided by Novozymes Biotechnology Co., Ltd.

[0064] Complex preparation: take 100ml of clear liquid, adjust the pH to 4.0, add 10ml of zinc chloride solution with a concentration of 0.1g / ml, preheat the electric furnace for 10min, filter...

Embodiment 2

[0066] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing and chop them quickly with a tissue grinder or chopping machine to avoid the raw materials from forming a gel.

[0067] Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1:5, dissolve in water, stir, adjust the pH to 7.5, add Bacillus subtilis neutral protease 1000U / g raw materials, and perform enzymolysis in a constant temperature water bath shaking shaker at 55°C React for 3 hours, shake once every 10 minutes. Then the hydrolyzate was heated to 100°C, kept for 10 min, cooled to room temperature, and centrifuged to obtain the supernatant for later use. The Bacillus subtilis neutral protease used in the hydrolysis was provided by Guangbo Nanning Pangbo Bioengineering Co., Ltd.

[0068] Complex preparation: take 100ml of clear liquid, adjust the pH to 4.0, add 10ml of zinc chloride solution with a concentration of 0.1g / ml, preheat the electric fur...

Embodiment 3

[0070] Pretreatment of animal raw materials: Weigh the leftovers of tilapia processing, and quickly chop them into fine pieces with a tissue grinder or chopping machine to avoid the formation of gelatinous raw materials.

[0071] Protein hydrolysis: take a certain amount of raw materials, according to the material: water = 1: 4, dissolve in water, stir, adjust the pH to 9, add alkaline protease 8000U / g raw materials, and carry out enzymatic hydrolysis reaction in a 55°C constant temperature water bath shaking shaker 2.5 h, shaking once every 10min. Then the hydrolyzed solution was heated to 100°C, kept for 10 min, cooled to room temperature, filtered to obtain the clear solution for later use. The alkaline protease used in the hydrolysis was Alcalase 2.4LFG, provided by Novozymes Biotechnology Co., Ltd.

[0072] Complex preparation: take 100ml of clear liquid, adjust the pH to 5.0, add 10ml of zinc oxide solution with a concentration of 0.1g / ml, preheat the electric furnace f...

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PUM

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Abstract

The invention discloses a zinc-polypeptide complex. The complex is prepared through animal origin and inorganic zinc at a weight ratio of (1:1)-(1:5). The preparation method of the complex comprises the following steps: adding polypeptide produced by hydrolyzing with alkaline protease or bacillus subtilis neutral proteinase to the animal origin; then coordinately reacting with inorganic zinc to obtain the complex, wherein the inorganic zinc is zinc oxide, zinc chloride or zinc carbonate, and zinc oxide or zinc carbonate is better; the animal origin is low-value fish and shellfish products and leftovers or waste produced by processing fish and shellfish. The invention also discloses application of zinc-polypeptide complex in preparation of medicines for preventing and controlling virus, inhibiting the growth of a cancer cell, enhancing the anti-infection capability of an animal to pathogene, and reducing the incidence of infectious diseases.

Description

technical field [0001] The invention belongs to the technical field of metal organic chemistry, and specifically relates to metal polypeptide complexes. More specifically, it is a polypeptide-zinc metal complex, its preparation method and its application. Background technique [0002] Zinc (Zn) is an essential trace element for humans and animals, known as the element of life. At present, organic zinc is mainly amino acid chelate, such as amino acid chelate of trace elements such as zinc, selenium or calcium. The amino acid chelate of edible trace elements can not only supplement the trace elements required by the human body or animals, but also simultaneously supplement the amino acids required by the human body or animals. State Intellectual Property Bureau disclosed an invention patent application "preparation method and application thereof of amino acid zinc" (application number 94114854.8) on April 2, 1997, and this application discloses a kind of preparation method o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/107A61K38/01A61P3/02A61P31/12A61P35/00A61P37/04
Inventor 李虹
Owner 天津拓飞科技有限公司
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