Method for judging lateolabrax japonicus aquatic water

A technology for cultivating water bodies and perch, applied in the fields of biochemical equipment and methods, determination/inspection of microorganisms, etc.

Inactive Publication Date: 2014-03-26
苏州市阳澄湖国家现代农业示范区发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For euryhaline fishes including perch, there are differences in the quality and price of fish in freshwater culture and seawater culture, so it is necessary to establish a technical method to identify whet

Method used

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  • Method for judging lateolabrax japonicus aquatic water

Examples

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Embodiment 1

[0024] (1) Extraction of total RNA: Put 50 mg of the gill tissue of sea bass cultured in seawater into a mortar, add liquid nitrogen, and grind the tissue into powder after the liquid nitrogen volatilizes. Put the tissue powder into a 1.5ml centrifuge tube, add Trizol reagent (purchased from Invitrogen) to lyse for 5-10 minutes, then add chloroform and let stand at room temperature for 15 minutes, then centrifuge in a centrifuge for 15 minutes (12000 rpm), take 500ul of supernatant, add isopropanol and let it stand for 10min, then centrifuge for 10min (12000 rpm), pour off the supernatant, add 75% alcohol to wash the precipitate, after centrifugation, pour off the supernatant to obtain the gill tissue of sea bass cultured in seawater Total RNA, add 40 μl DEPC water to dissolve and set aside;

[0025] (2) Synthesis of the first strand of cDNA: using the M-MLV reverse transcriptase kit purchased from Promega. Take the extracted total RNA with a volume of more than 1 μl, mix it ...

Embodiment 2

[0030] The specific steps are the same as in Example 1, except that the tissue used is the kidney of sea bass cultured in seawater.

Embodiment 3

[0032] The specific steps are the same as in Example 1, except that the tissue used is the gills of freshwater cultured perch.

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Abstract

The invention discloses a method for judging lateolabrax japonicus aquatic water. The method specifically comprises the following steps: extracting total RNA (Ribonucleic Acid) from lateolabrax japonicus tissues to be judged, and synthesizing a first cDNA (complementary Deoxyribonucleic Acid) chain; amplifying a prolactin receptor via a PCR (Polymerase Chain Reaction); observing whether a prolactin receptor expression strap exists on the gel electrophoresis picture of a PCR amplification product or not in order to judge whether lateolabrax japonicus under detection is cultured in fresh water or sea water. The method disclosed by the invention is simple and accurate, and can be applied to supervision in the field of aquatic products.

Description

technical field [0001] The invention relates to a method for judging the water body of sea bass culture, which can determine whether the detected sea bass is a product of fresh water or marine culture. Background technique [0002] Perch ( Lateolabrax japonicus ) is an economic fish in the middle and lower layers of coastal waters, and it is also the main cultured species in seawater at present. This fish has few diseases, fast growth, easy fishing, high yield, good profit, tender meat, delicious taste, rich nutrition, and has the functions of promoting muscle growth, nourishing blood, nourishing and strengthening the body, etc. Its health care effect is deeply loved by people. After desalination and domestication, sea bass has also begun to be farmed in large areas in fresh water. [0003] For euryhaline fish including perch, there are differences in the quality and price of fish in freshwater culture and seawater culture, so it is necessary to establish a technical metho...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/158
Inventor 唐雪生王国清鲍宝龙张铖陈文君刘小玉
Owner 苏州市阳澄湖国家现代农业示范区发展有限公司
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