Technology for preparing antibacterial peptide

An antimicrobial peptide and process technology, applied in the field of preparation of antimicrobial peptide, can solve the problems of long production cycle, narrow antibacterial spectrum and high production cost

Inactive Publication Date: 2014-04-02
潍坊市东方海洋生物科技研发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention proposes a process for preparing antimicrobial peptides, which solves the probl

Method used

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  • Technology for preparing antibacterial peptide

Examples

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Embodiment 1

[0032] A process for preparing antimicrobial peptides, comprising the steps of:

[0033] 1) Mix 2 parts by weight of Bacillus licheniformis, 0.5 parts by weight of Bacillus cereus, and 1 part by weight of Bacillus natto;

[0034] 2) Anaerobic fermentation of the above-mentioned homogeneously mixed materials at 36°C for 6 hours to obtain reserved seeds;

[0035] 3) Anaerobic fermentation of the above-mentioned reserved seeds in a fermenter at 36°C for 27 hours;

[0036] 4) Press filter the above-mentioned fermented reserve seeds through plate and frame;

[0037] 5) The above-mentioned filtered reserve seeds are spray-dried to obtain the antimicrobial peptide.

[0038] The plate and frame filter press used in the step 4) consists of alternately arranged filter plates and filter frames to form a set of filter chambers. The surface of the filter plate has grooves, and its protruding parts are used to support the filter cloth. , There are through holes on the corners of the filt...

Embodiment 2

[0041] A process for preparing antimicrobial peptides, comprising the steps of:

[0042] 1) Mix 3 parts by weight of Bacillus licheniformis, 1 part by weight of Bacillus cereus, and 1.5 parts by weight of Bacillus natto;

[0043] 2) Anaerobic fermentation of the above-mentioned homogeneously mixed materials at 38°C for 8 hours to obtain reserved seeds;

[0044] 3) Anaerobic fermentation of the above-mentioned reserved seeds in a fermenter at 38°C for 32 hours;

[0045] 4) Press filter the above-mentioned fermented reserve seeds through plate and frame;

[0046] 5) The above-mentioned filtered reserve seeds are spray-dried to obtain the antimicrobial peptide.

[0047] The plate and frame filter press used in the step 4) consists of alternately arranged filter plates and filter frames to form a set of filter chambers. The surface of the filter plate has grooves, and its protruding parts are used to support the filter cloth. , There are through holes on the corners of the filt...

Embodiment 3

[0050] A process for preparing antimicrobial peptides, comprising the steps of:

[0051] 1) Mix 4 parts by weight of Bacillus licheniformis, 1.5 parts by weight of Bacillus cereus, and 2 parts by weight of Bacillus natto;

[0052] 2) Anaerobic fermentation of the above-mentioned homogeneously mixed materials at 39°C for 10 hours to obtain reserved seeds;

[0053] 3) Anaerobic fermentation of the above-mentioned reserved seeds in a fermenter at 39°C for 37 hours;

[0054] 4) Press filter the above-mentioned fermented reserve seeds through plate and frame;

[0055] 5) The above-mentioned filtered reserve seeds are spray-dried to obtain the antimicrobial peptide.

[0056] The plate and frame filter press used in the step 4) consists of alternately arranged filter plates and filter frames to form a set of filter chambers. The surface of the filter plate has grooves, and its protruding parts are used to support the filter cloth. , There are through holes on the corners of the filt...

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Abstract

The invention provides a technology for preparing an antibacterial peptide. The technology comprises the following steps of mixing 2-4 parts by weight of bacillus licheniformis, 0.5-1.5 parts by weight of bacillus cereus and 1-2 parts by weight of bacillus natto to obtain a uniform mixture, carrying out mixture fermentation at a temperature of 36-39 DEG C for 6-10h to obtain storage strains, carrying out storage strain fermentation in a fermentation tank at a temperature of 36-39 DEG C for 27-37h, carrying out plate-frame pressure filtration on the fermented storage strains, and carrying out spray drying on the filtered storage strains to obtain the antibacterial peptide. The triple strain fermentation product obtained by the technology has effects on hemolytic streptococcocci, pneumococcocci, enterococcus, corynebacterium diphtheria, bacillus anthracis, gram-positive anoxic bacillus, achalme bacillus, clostridium tetani, clostridium difficile, eubacterium and lactic acid bacillus. The technology can shorten a production period by 1/3 and reduce a use cost by 60%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a process for preparing antibacterial peptides. Background technique [0002] At present, the antimicrobial peptide products in the market have a narrow antibacterial spectrum, and those fermented by a single strain in the antibacterial range are effective against hemolytic streptococcus and pneumococcus, but not effective against others, resulting in a long production cycle and high production costs. Contents of the invention [0003] The invention proposes a process for preparing antimicrobial peptides, which solves the problems of narrow antibacterial spectrum, long production cycle and high production cost of antimicrobial peptide products in the prior art. [0004] The technical scheme of the present invention is achieved in that a kind of technique for preparing antimicrobial peptide comprises steps: [0005] 1) Mix 2-4 parts by weight of Bacillus licheniformis, 0.5-1.5 part...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/10C12R1/085C12R1/07
Inventor 张姚滨
Owner 潍坊市东方海洋生物科技研发中心
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