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Method for extracting rebaudioside D from stevioside crystalline mother liquor sugar

A technology for crystallizing mother liquor and stevioside, applied in the field of extracting rebaudioside D, can solve problems such as separation, and achieve the effects of simple process, low energy consumption and easy realization

Active Publication Date: 2014-04-09
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because the structure of each component in the stevioside crystallization mother liquor sugar is similar, there is no simple and effective method for separating RD from the stevioside crystallization mother liquor sugar in the prior art

Method used

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  • Method for extracting rebaudioside D from stevioside crystalline mother liquor sugar
  • Method for extracting rebaudioside D from stevioside crystalline mother liquor sugar
  • Method for extracting rebaudioside D from stevioside crystalline mother liquor sugar

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specific Embodiment approach

[0024] Specific embodiments: The method for extracting rebaudioside D in stevioside in the present invention will be described below through specific embodiments. In addition, the embodiments should be considered as illustrative rather than limiting the scope of the invention, the spirit and scope of which is defined only by the claims. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the crystallization conditions in these embodiments also belong to the protection scope of the present invention.

[0025] The assay method of total steviosides in the present invention is as follows:

[0026] HPLC quantitative determination method (JECFA2010 meeting standard)

[0027] Detection steps: detect the content of each glycoside according to the following liquid chromatography conditions.

[0028] Reagent: acetonitrile, transmittance >95% at 210nm.

[0029] Standard specifications:

...

Embodiment 1

[0061] Take 100g of stevioside crystallization mother liquid sugar, add 300ml of solvent, water:methanol=1:7, the purity of methanol is 99.9%, put it in a three-necked flask after fully mixing, the temperature of the water bath is controlled at 50 degrees Celsius, the stirring speed is 20rpm / min, the time For 1 hour, the crystallization mixture was suction-filtered with a Buchner funnel, the obtained crystals were washed with a solvent, the solid-to-liquid ratio was 1:4, and dried overnight at 60°C to obtain 43 g of a dry sample. The liquid phase measurement result is:

[0062]

[0063] The RD content in the product can be raised to more than 40% by adopting the method of Example 1.

Embodiment 2

[0065] Take 200g of stevioside crystallization mother liquid sugar, add 400ml of solvent, water:methanol=1:9, the purity of methanol is 99.9%, put it in a three-necked flask after fully mixing, the temperature of the water bath is controlled at 65 degrees Celsius, and the stirring speed is 60rpm / min. After 4 hours, the crystallization mixture was suction-filtered with a Buchner funnel, and the obtained crystals were washed with a solvent at a solid-to-liquid ratio of 1:2, and dried overnight at 60°C to obtain 101 g of a dry sample. The liquid phase measurement result is:

[0066]

[0067] The RD content in the product can be raised to 50% by adopting the method of Example 2.

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Abstract

The invention discloses a method for extracting rebaudioside D from stevioside crystalline mother liquor sugar, and belongs to the fields of bioengineering and novel sweetening agents. The method mainly comprises the following steps: dissolving primary crystal transformation mother liquid sugar after RA extraction into a mixed organic solvent of water and methanol with the ratio of 1:(7-10); keeping the stirring speed at 20-100rpm / min at 50-70 DEG C for 1-10 hours to crystallize; removing impurities in the mother liquor after washing a solid substance by solid-liquid separation; baking to obtain a high-purity rebaudioside D (RD) product. The ratio of the RD in the raw material sample can be improved to over 50% from 20-30% by primary crystallization, and the RD product with high purity can be obtained by repeating for a plurality of times on the basis, so that a foundation is established for production of the high-purity RD product. The method is simple in technology, low in energy consumption, and easy to produce, and is used for stirring and controlling temperature in production. The mother liquor can be recycled after being concentrated, and the solvent can be recycled after being distilled. Thus, good economic benefits and low environmental pollution are achieved.

Description

Technical field: [0001] The invention belongs to the fields of bioengineering and novel sweeteners, and in particular relates to a method for extracting rebaudioside D from stevioside crystallization mother liquid sugar. Background technique: [0002] Stevia is a new type of natural sweetener extracted from the herb Stevia rebaudiana of Compositae. It has the characteristics of high sweetness and low calorie. Its sweetness is 200-350 times that of sucrose, and its calorific value is only 1 / 300 of that of sucrose. Among the 9 sweet components of stevioside, the relative content of stevioside (ST), rebaudioside A (RA) and rebaudioside C (RC) is relatively high, accounting for more than 90% in total, which determine the taste of stevioside main component of the quality. The sweetness of RA is the highest, equivalent to 450 times that of sucrose, and the sweetness characteristics are also close to that of sucrose; the sweetness of stevioside is equivalent to 300 times that of ...

Claims

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Application Information

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IPC IPC(8): C07H15/256C07H1/06
Inventor 乔长晟吕爱弟楼鹏
Owner 天津北洋百川生物技术有限公司
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