A method for preparing surface-enhanced infrared spectroscopic silver substrate
An infrared spectroscopy and surface enhancement technology, applied in the field of surface enhanced infrared spectroscopy, can solve the problems of affecting the test results of surface enhanced infrared spectroscopy, the enhancement effect is not significant, and the substrate preparation cost is high, and achieves significant enhancement effect, low equipment requirements, and manufacturing costs. low effect
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Embodiment 1
[0029] Example 1: Preparation of Surface Enhanced Infrared Spectroscopy Silver Substrate
[0030] Clean the surface of the copper sheet first, and roughen the cleaned copper sheet in concentrated nitric acid for 5 seconds; soak the roughened copper sheet in 6M hydrochloric acid for 10 minutes, and then clean it with ultrapure water; the copper sheet treated with hydrochloric acid The chip is put into 15mM silver nitrate solution, copper and silver ions undergo substitution reaction, the reaction time is about 30 minutes, then cleaned with ultrapure water, and then dried with nitrogen, so that the surface enhanced infrared spectrum silver substrate is obtained.
[0031] attached figure 1 It is the TEM picture of the above surface-enhanced infrared spectrum silver substrate. It can be seen that the silver aggregates on the substrate are in the shape of pine dendrites, including pine needles and branches. The size of the branches is on the order of microns, and the gaps between ...
Embodiment 2
[0033] Example 2: Preparation of Surface Enhanced Infrared Spectroscopy Silver Substrate
[0034] Clean the surface of the copper sheet first, and roughen the cleaned copper sheet in concentrated nitric acid for 10 seconds; then wash it with ultrapure water; put the roughened copper sheet into 15mM silver nitrate solution, and the copper and silver ions will Replacement reaction, the reaction time is about 60 minutes, then washed with ultrapure water, and then dried with nitrogen, so as to obtain the surface-enhanced infrared spectrum silver substrate.
[0035] Put the above surface-enhanced infrared spectrum silver substrate into 1mM ethanol solution of p-mercaptopyridine (MPy) and soak for 6 hours; use the silver substrate adsorbed with MPy to measure the reflection infrared spectrum of MPy, the test results are shown in the attached Figure 4 , it can be seen that the characteristic peaks of MPy are significantly enhanced.
Embodiment 3
[0036] Example 3: Preparation of Surface Enhanced Infrared Spectroscopy Silver Substrate
[0037] Clean the surface of the copper sheet first, and roughen the cleaned copper sheet in concentrated nitric acid for 5 seconds; soak the roughened copper sheet in 6M hydrochloric acid for 10 minutes, and then clean it with ultrapure water; the copper sheet treated with hydrochloric acid Put the chip into 15mM silver nitrate solution, copper and silver ions undergo substitution reaction, the reaction time is about 40 minutes, then wash with ultra-pure water, and then dry with nitrogen, so that the silver substrate for surface-enhanced infrared spectroscopy is obtained.
[0038] Put the above-mentioned surface-enhanced infrared spectrum silver substrate into 1mM methylene blue (MB) ethanol solution and soak for 6 hours; use the silver substrate adsorbed with MB to measure the reflection infrared spectrum of MB, the test results are shown in the appendix Figure 5 , it can be seen that ...
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