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Resolution testing standard board manufacturing method for super-resolution fluorescence microscope system

A technology of super-resolution fluorescence and microscopic systems, which is applied in the field of manufacturing super-resolution fluorescent microscopic system resolution test standard plates, can solve the problems of inapplicable observation of living samples, cumbersome process, slow observation speed, etc., and achieve simple production methods Reliable, wide excitation spectrum, and improved precision

Active Publication Date: 2014-04-09
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

This type of technology has certain defects: during the observation process, it is necessary to repeatedly activate-quench fluorescent molecules, resulting in a slow observation speed, which is not suitable for observing living samples
[0005] At present, the resolution detection of super-resolution fluorescence microscopy mainly uses fluorescent nanospheres, but the process of this method is relatively cumbersome.

Method used

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  • Resolution testing standard board manufacturing method for super-resolution fluorescence microscope system
  • Resolution testing standard board manufacturing method for super-resolution fluorescence microscope system
  • Resolution testing standard board manufacturing method for super-resolution fluorescence microscope system

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Embodiment Construction

[0035] The technical innovation of the present invention is: using nano-processing technology and nano-particle self-assembly technology to make fluorescent quantum dot lines with a line width of 20nm-100nm as a fluorescent super-resolution standard plate, which can be directly used for super-resolution system testing.

[0036] The specific structure schematic diagram of the first method of making the fluorescent super-resolution standard plate in the present invention is as follows Figure 1a to Figure 1c shown, including the following steps:

[0037] Spin coating a photoresist layer 104 on the surface of the substrate 100, the substrate 100 may be Si or quartz;

[0038] Such as Figure 1a As shown, an equidistant photoresist line structure of 20nm-100nm is formed on the photoresist layer 104 by electron beam lithography technology, so that the substrate 100 is exposed, and the thickness of the electron beam photoresist is between 50nm-150nm. Control the steepness of the pho...

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Abstract

The invention discloses a resolution testing standard board manufacturing method for a super-resolution fluorescence microscope system. A nano-manufacturing technology and a nanoparticle self-assembling technology are used for manufacturing a fluorescent quantum dot line with a width of 20 to 100nm as a fluorescent super-resolution standard board. At present, a main means for detecting the resolution of a super-resolution fluorescence microscope is fluorescent nanospheres, but is very complex. The manufacturing method is simple and reliable, excellent fluorescent characteristics are ensured, and the manufactured super-resolution standard board can be directly used for testing the super-resolution fluorescence microscope system.

Description

technical field [0001] The invention relates to the technical field of resolution detection of a super-resolution fluorescence microscope system, in particular to a method for manufacturing a resolution test standard plate of a super-resolution fluorescence microscope system. Background technique [0002] In biomedicine and other fields, in order to better understand the process of life and the mechanism of disease, it is necessary to observe the precise positioning and distribution of fine structures such as organs in cells, and to clarify how biological macromolecules such as proteins form the basic structure of cells. How active factors regulate the main life activities of cells, etc. [0003] The characteristic scales of the above systems are at the nanometer level, and conventional optical microscopy techniques can no longer meet the observation requirements. In order to further improve the resolution of the optical microscope, the early discussions were mainly based o...

Claims

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Application Information

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IPC IPC(8): G01N21/64G03F7/20
Inventor 张志强蒋克明黎海文张运海吴一辉
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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