Anticoagulation coating material and preparation method thereof

A coating material and anti-coagulation technology, applied in the field of anti-coagulation coating materials and their preparation, can solve the problems of unstable anti-coagulation effect, not long-term, poor biocompatibility, etc., and achieve a significant anti-coagulation effect. , Not easy to be deactivated, the effect of stable structure

Inactive Publication Date: 2015-05-20
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides an anticoagulant coating material, the purpose of which is to combine the anticoagulant factor hirudin, which has a small molecular weight and stable anticoagulant effect, with a good biological phase. The capacitive sodium hyaluronate gel network is covalently bonded, thereby solving the technical problems of unstable or short-term anticoagulant effect and poor biocompatibility of current anticoagulant coating materials

Method used

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  • Anticoagulation coating material and preparation method thereof
  • Anticoagulation coating material and preparation method thereof

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preparation example Construction

[0036] A kind of preparation method of anticoagulation coating material provided by the invention comprises the following steps:

[0037] (1) Prepare 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a concentration of 0.1mol / L and adjust the pH value to 6.4.

[0038] (2) Preparation of sodium hyaluronate solution:

[0039] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4), and dissolve it fully to obtain sodium hyaluronate solution. The sodium hyaluronate solution has a concentration of 1 mg / ml to 20 mg / ml. Magnetic stirring can be used to accelerate the dissolution of sodium hyaluronate, stirring for 12 hours. The prepared sodium hyaluronate solution can be sealed and frozen for future use.

[0040](3) Preparation of hirudin solution:

[0041] Take the hirudin in the MES buffer, so that the hirudin is uniformly dispersed in the MES buffer. In order to increase the solub...

Embodiment 1

[0050] An anticoagulant coating material comprises sodium hyaluronate and hirudin, the sodium hyaluronate forms a gel network, and the hirudin is covalently bonded to the sodium hyaluronate through an amide bond. The mass ratio of the hirudin to the sodium hyaluronate is 1:1. The anticoagulant coating material also includes a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) .

[0051] The preparation method of described anticoagulant coating material, comprises the following steps:

[0052] (1) Prepare 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a concentration of 0.1mol / L and adjust the pH value to 6.4.

[0053] (2) Prepare sodium hyaluronate solution.

[0054] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4), and dissolve it fully to obtain sodium hyaluronate solution. The concentration of sodium hyaluronate ...

Embodiment 2

[0066] An anticoagulant coating material comprises sodium hyaluronate and hirudin, the sodium hyaluronate forms a gel network, and the hirudin is covalently bonded to the sodium hyaluronate through an amide bond. The mass ratio of the hirudin to the sodium hyaluronate is 1:3. The anticoagulant coating material also includes a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) .

[0067] The preparation method of described anticoagulant coating material, comprises the following steps:

[0068] (1) Prepare 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution with a concentration of 0.1mol / L and adjust the pH value to 6.4.

[0069] (2) Prepare sodium hyaluronate solution.

[0070] Take sodium hyaluronate (HA, molecular weight about 2 million) in 2-(N-morpholine) ethanesulfonic acid buffer solution (MES, 0.1M, pH=6.4), and dissolve it fully to obtain sodium hyaluronate solution. The concentration of sodium hyaluronate ...

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Abstract

The invention discloses an anticoagulation coating material and a preparation method thereof. The anticoagulation coating material comprises sodium hyaluronate and hirudin, wherein the sodium hyaluronate is formed into a gel network; the hirudin is in covalent binding with the sodium hyaluronate. The preparation method of the anticoagulation coating material comprises the following steps: (1) preparing an MES (Fatty Acid Methyl Ester Sulfonate) buffer solution; (2) preparing a sodium hyaluronate solution with the concentration of 1mg / ml to 20mg / ml; (3) preparing a hirudin solution with the concentration of 1mg / ml to 10mg / ml; (4) uniformly mixing up the two solutions so as to enable the mass ratio of the hirudin to the sodium hyaluronate to be 1:1 to 1:10; (5) adding a crosslinking agent into the mixture solution obtained in the step (4), uniformly mixing up so as to covalently crosslink the hirudin and the sodium hyaluronate. The anticoagulation coating material disclosed by the invention is high in anticoagulation activity, stable in effect, long in lasting time, good in biocompatibility, simple in preparation method, gentle in condition and applicable to large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and more specifically relates to an anticoagulant coating material and a preparation method thereof. Background technique [0002] Anticoagulant biomedical materials are an important class of biological materials, which are widely used in medical materials in contact with human blood and tissues, such as hemodialysis systems, extracorporeal circulation systems, artificial heart valves, cardiac pacemakers, artificial blood vessels , vascular stents, surgical lines and catheters, etc. In the 21st century, the morbidity and mortality of cardiovascular diseases are on the rise, and the prevention and treatment of cardiovascular diseases has become the focus of medical circles at home and abroad. Anticoagulant biomedical materials have important application value. [0003] At present, there are anticoagulant coatings, mainly using heparin as an anticoagulant factor, and heparin is fixed on the su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L33/08A61L33/12A61L33/04
Inventor 马军方堃雷冰雨花城
Owner HUAZHONG UNIV OF SCI & TECH
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