Novel mixed fungicide for fermenting yogurt
A technology of mixed bacterial agents and mixed starters, applied in the fields of application, milk preparations, dairy products, etc., can solve the problems of unremarkable typical flavor of yogurt, unbalanced ratio of bacilli and cocci, poor coagulation state of yogurt, etc., to achieve a suitable and refreshing sour taste , high acid production, and the effect of solving poor solidification state
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Embodiment 1
[0032] The concrete breeding process of described plantarum lactobacillus is as follows:
[0033] 1. Diethyl Sulfate (DES) Mutation Breeding
[0034] (1) Take a ring of Lactobacillus plantarum on the inclined surface of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL liquid MRS xylan medium, and incubate at 200rpm at 40°C for about 12h, so that the bacteria are in the right condition. Early growth period.
[0035] (2) Take 5mL of bacterial liquid, centrifuge at 5000rpm for 10min to collect bacterial cells, and wash twice with normal saline.
[0036] (3) Dilute with pH7.0 phosphate buffer to 107 / mL bacterial suspension.
[0037] (4) Take 32mL of pH7.0 potassium phosphate buffer solution, 8mL of bacterial suspension, and 0.4mL of DES and mix thoroughly in a 150mL Erlenmeyer flask pre-placed in the rotor, so that the final concentration of DES is 1% (v / v).
[0038] (5) React in a shaker at 30°C at 150rpm for 30min, take 1mL of th...
Embodiment 2
[0054] The composition of the mixed bacteria agent for yogurt fermentation is as follows: the mixed starter agent is composed of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animal;
[0055] The mixed bacterial agent: the mass ratio of Lactobacillus plantarum, Lactobacillus bulgaricus and Bifidobacterium animalis 1:0.5:0.1;
[0056] A method for preparing yogurt, comprising the steps of:
[0057] Raw material processing: acceptance, sterilization, storage, standardization, batching, degassing and homogenization of raw milk;
[0058] Secondary sterilization: After homogenization, the material is sterilized through a heat exchanger under the condition of 95°C for 300s;
[0059] Cooling and inoculation: cool down the sterilized material to 43-45°C, add the mixed working fermentation bacteria agent, and the addition amount is 2% of the material.
[0060] Stirring fermentation: After the inoculation work is completed, start stirring, stop stirring after 1...
Embodiment 3
[0063] Embodiment 3: The composition of the mixed bacteria agent for yogurt fermentation is as follows: the mixed starter agent is composed of plant Lactobacillus, Lactobacillus bulgaricus and animal Bifidobacterium; the mixed bacteria agent: plant Lactobacillus, bulgaricus and animal Bifidobacterium The mass ratio of mycobacteria is 1:0.2:0.1.
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