Method for extracting cordyceps substrate polysaccharide from waste cordyceps militaris culture medium and application of extract

A technology of waste substrate and Cordyceps militaris, which is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve the problems that the ingredients are not subdivided and have no activity, and achieve pure chemical composition and exact activity. Effect

Active Publication Date: 2014-04-30
NANJING XIAOZHUANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the biological extracts such as polysaccharides retained in the prior art are crude polysaccharides, and the components in the crude polysaccharides have not been subdivided, some have biological activity, and some do not have relevant activities

Method used

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  • Method for extracting cordyceps substrate polysaccharide from waste cordyceps militaris culture medium and application of extract
  • Method for extracting cordyceps substrate polysaccharide from waste cordyceps militaris culture medium and application of extract

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 The extraction method of Cordyceps militaris matrix polysaccharide provided by the present invention

[0032] 1) Raw material handling

[0033] Take the waste substrate of the Cordyceps militaris culture medium (the culture medium raw material is rice), pulverize after drying, pass through a 60-mesh sieve, and set aside;

[0034] 2) Enzyme extraction

[0035] Add water to the matrix powder obtained in step 1) according to the ratio of matrix powder: distilled water = 1:20, adjust the pH value to 4.5, add 500 U of cellulase per gram of Cordyceps dry powder, stir at 50°C for 2 hours;

[0036] 3) Microwave extraction

[0037] Put the enzymatic hydrolyzate obtained in step 2) in a microwave field for 5 minutes, and the power of the microwave field is 480W;

[0038] 4) In addition to starch and protein

[0039]For the extract obtained in step 3) microwave treatment, heat at 90°C for 10 minutes to inactivate the enzyme, then add a medium-temperature amyl...

Embodiment 2

[0046] Example 2 Extraction method of Cordyceps militaris matrix polysaccharide provided by the present invention (compound enzyme extraction method)

[0047] 1) Raw material handling

[0048] Take the waste substrate of the Cordyceps militaris culture medium (the culture medium raw material is rice), crush it after drying, pass through a 100-mesh sieve, and set aside;

[0049] 2) Enzyme extraction

[0050] Take 100g of matrix powder and add 250ml of water, add cellulase at 500U per gram of Cordyceps dry powder, and at the same time add pectinase at 2500U per gram of Cordyceps dry powder, adjust the pH to 6.0, and stir at 50°C for 1 hour for enzymolysis;

[0051] 3) Enzymatic removal of starch and protein

[0052] Adjust the pH value of the enzymolysis solution obtained in step 2) to 7.0, add a certain amount of medium-temperature amylase, hydrolyze at 80°C for 60 minutes to remove starch, and inactivate the enzyme at 90°C for 5 minutes. / L of hydrochloric acid to adju...

Embodiment 3

[0057] Example 3 Extraction method of Cordyceps militaris matrix polysaccharide provided by the present invention (microwave combined with enzymatic extraction)

[0058] 1) Raw material handling

[0059] Get the waste matrix of the Cordyceps militaris wheat culture medium, pulverize after drying, pass through a 100 mesh sieve, and set aside;

[0060] 2) Complex enzyme extraction

[0061] Add cellulase at 1000U per gram of Cordyceps dry powder, and at the same time add pectinase at 1500U per gram of Cordyceps dry powder, adjust the pH value to 5.0, stir and enzymolyze at 55°C for 1 hour, then heat to inactivate the enzyme;

[0062] 3) Microwave extraction

[0063] The extract obtained in step 2) was placed in a microwave field for 4 minutes, and the power of the microwave field was 600W;

[0064] 4) Remove starch and protein

[0065] Heat the extract obtained in step 3) at 95°C for 10 minutes to inactivate the enzyme. After cooling, it was centrifuged to obtain a supern...

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Abstract

The invention relates to a method for extracting cordyceps substrate polysaccharide from a waste cordyceps militaris culture medium and an application of the extract. The method comprises the experimental steps of performing enzymolysis and microwave and centrifugal separation, then adding ethanol for deposition, then removing starch and protein, performing gel column purification and the like to realize extraction of the cordyceps substrate polysaccharide from the waste cordyceps militaris culture medium. The polysaccharide extract is single in component, the polysaccharide content is more than 90%, and compared with the current existing liver-protecting anti-alcohol medicaments and health foods, the extract has the characteristics of more precise activity, purer chemical components and the like.

Description

technical field [0001] The invention relates to the field of biological products, more specifically, relates to a method for extracting cordyceps matrix polysaccharides from waste Cordyceps militaris culture substrates and the application of the extracts. Background technique [0002] Alcoholic liver injury is a liver damage disease caused by excessive drinking. With the development of the current society, people's alcohol consumption has begun to rise sharply, and liver damage caused by alcohol abuse and alcohol dependence has become the second leading cause of liver damage after viral hepatitis. Alcoholic liver injury can be divided into acute alcoholic liver injury and chronic alcoholic liver injury. Acute alcoholic liver injury is liver function damage caused by excessive alcohol intake at one time, manifested as symptoms of acute hepatitis such as loss of appetite, nausea and vomiting, and liver discomfort. [0003] At present, the treatment methods for alcoholic l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
Inventor 华春汪振炯王仁雷吴雨龙周峰江海涛周泉澄毛善国丁春霞陈晓鹏王雪池月兰
Owner NANJING XIAOZHUANG UNIV
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